LOCUS       sequence-256926-        6335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 179_pAAV-ProD10-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        694..725
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      779..788
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             785..1711
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1715..1717,1718..1720,1721..2434)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1715..1717,1718..1720,1721..2431)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1760..1781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2021..2040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2462..3050
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2515..2535)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2933..2944)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3082..3558
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3281..3300)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3598..3738
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3598..3727
                     /label=AAV2 ITR
     rep_origin      3813..4268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3900..3919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4110..4131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4285..4304)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4404..4426
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4464..4482)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4550..4654
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4655..4723,4724..5515)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4873..4892)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5686..6274
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6175..6194
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatcctg gggtttaaac aggactaggt ccactgggag
      181 aagaggaagt caaacaggac tagtcatcga taggctagcc agcttaatta atcagatagg
      241 aagactagtc agcatcagga aagcttacta cagtatgtat ggtgtgctag tgagctagag
      301 ctagagcaaa gccggattac ctccaaacat gctggggaag aattgactac acttctacct
      361 aaacagtatg ccagaagaga aacagaagtg tggtgccttc ttagcgtgac tcagagtacc
      421 tatgaaagct actagcccgt gttacaagtg actagaaaca ctcaggcatc ggtgctgaaa
      481 tcggccaagg agacctgtgt caccatttct gtttagaatc cagtaaaacc agcttagttg
      541 acattacagc tcaggcttgg gttgatttct aaagttcaga gccaatcaaa agtcaccaga
      601 aaagcccaga gcctgggtgt gttgtgttgc tcagtggtcg agctcctgct gggaggatgg
      661 ggagggatcc tcacatggtc ctgctggagt tagtagaggg tatataatgg aagctcgact
      721 tccagctatc acatccactg tgttgttgtg aactggaatc cactataggc caggatccgc
      781 caccatggat tatggaggcg ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
      841 cccagtagtc gtcaatggct ctgtacttgt gcctgaggac cagtgttact gcgcgggctg
      901 gattgagtcg cgtggcacaa acggtgccca aacggcgtcg aacgtgctgc aatggcttgc
      961 tgctggcttc tccatcctac tgcttatgtt ttacgcctac caaacatgga agtcaacctg
     1021 cggctgggag gagatctatg tgtgcgctat cgagatggtc aaggtgattc tcgagttctt
     1081 cttcgagttt aagaacccgt ccatgctgta tctagccaca ggccaccgcg tccagtggtt
     1141 gcgttacgcc gagtggcttc tcacctgccc ggtcatttgc attcacctgt caaacctgac
     1201 gggcttgtcc aacgactaca gcaggcgcac catgggtctg cttgtgtctg atattggcac
     1261 aattgtgtgg ggcgccactt ccgccatggc caccggatac gtcaaggtca tcttcttctg
     1321 cctgggtctg tgttatggtg ctaacacgtt ctttcacgct gccaaggcct acatcgaggg
     1381 ttaccacacc gtgccgaagg gccggtgtcg ccaggtggtg actggcatgg cttggctctt
     1441 cttcgtatca tggggtatgt tccccatcct gttcatcctc ggccccgagg gcttcggcgt
     1501 cctgagcgtg tacggctcca ccgtcggcca caccatcatt gacctgatgt cgaagaactg
     1561 ctggggtctg ctcggccact acctgcgcgt gctgatccac gagcatatcc tcatccacgg
     1621 cgacattcgc aagaccacca aattgaacat tggtggcact gagattgagg tcgagacgct
     1681 ggtggaggac gaggccgagg ctggcgcggt acccatggtg agcaagggcg aggagctgtt
     1741 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     1801 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     1861 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     1921 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     1981 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2041 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2101 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     2161 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     2221 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     2281 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     2341 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     2401 gatcactctc ggcatggacg agctgtacaa gtgataagaa ttcgatatca agcttatcga
     2461 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2521 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2581 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2641 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2701 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2761 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2821 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2881 cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2941 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3001 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     3061 agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     3121 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     3181 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     3241 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     3301 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     3361 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     3421 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     3481 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     3541 gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
     3601 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     3661 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     3721 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     3781 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
     3841 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
     3901 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3961 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4021 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4081 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4141 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
     4201 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4261 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4321 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4381 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4441 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4501 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4561 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4621 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4681 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4741 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4801 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4861 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4921 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4981 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5041 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5101 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5161 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5221 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5281 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5341 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5401 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5461 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5521 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5581 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacccaca ccgaactgag
     6061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6301 gttcctggcc ttttgctggc cttttgctca catgt
//
