LOCUS       sequence-256929-        6441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 108_pAAV-ProC18-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   312..441
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        993..1189
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1040..1175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1196..1205
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1202..2128
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2132..2134,2135..2137,2138..2851)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2132..2134,2135..2137,2138..2848)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2177..2198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2438..2457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2785..2806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2879..3467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2932..2952)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3350..3361)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3499..3975
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3698..3717)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4015..4155
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4015..4144
                     /label=AAV2 ITR
     rep_origin      4230..4685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4317..4336)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4527..4548
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4702..4721)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4821..4843
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4881..4899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4967..5071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5072..5140,5141..5932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5290..5309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6103..6441,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc
      361 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      421 actccatcac taggggttcc tgcggccgca cgcgttggcc ggctctcgat ggtaattatc
      481 taacaaaatc gacgacggta attatctcta aaagtgtgtt tcggtaatta tcttgatgct
      541 taccgaacgg taattatctg ggatctacag ttgcggtaat tatctgtaga ggagtggggc
      601 ggtaattatc ttcaaccgaa cagcatggta attatctcat tcttcggatt tgggtaatta
      661 tctcggaatt tataggtggg taattatctt tagaatttgc tcacggtaat tatctaatga
      721 tcagcaagcc ggtaattatc ttgctcctca tgaggtggta attatctagc aggatttaca
      781 atggtaatta tctgctgtgt ggacatctgg taattatctc tgtcataagg acctggtaat
      841 tatcttgacc gtacggcggg ggtaattatc tacagtatta tgaaacggta attatcttcg
      901 tggcattcgg ggggtaatta tctgccgtgg gactcagagg taattatcta tatgtgttga
      961 aactggtaat tatctgctcg agatctgcga tctgcatctc aattagtcag caaccatagt
     1021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     1081 ccatcgctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     1141 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag gatccgccac
     1201 catggattat ggaggcgccc tgagtgccgt tgggcgcgag ctgctatttg taacgaaccc
     1261 agtagtcgtc aatggctctg tacttgtgcc tgaggaccag tgttactgcg cgggctggat
     1321 tgagtcgcgt ggcacaaacg gtgcccaaac ggcgtcgaac gtgctgcaat ggcttgctgc
     1381 tggcttctcc atcctactgc ttatgtttta cgcctaccaa acatggaagt caacctgcgg
     1441 ctgggaggag atctatgtgt gcgctatcga gatggtcaag gtgattctcg agttcttctt
     1501 cgagtttaag aacccgtcca tgctgtatct agccacaggc caccgcgtcc agtggttgcg
     1561 ttacgccgag tggcttctca cctgcccggt catttgcatt cacctgtcaa acctgacggg
     1621 cttgtccaac gactacagca ggcgcaccat gggtctgctt gtgtctgata ttggcacaat
     1681 tgtgtggggc gccacttccg ccatggccac cggatacgtc aaggtcatct tcttctgcct
     1741 gggtctgtgt tatggtgcta acacgttctt tcacgctgcc aaggcctaca tcgagggtta
     1801 ccacaccgtg ccgaagggcc ggtgtcgcca ggtggtgact ggcatggctt ggctcttctt
     1861 cgtatcatgg ggtatgttcc ccatcctgtt catcctcggc cccgagggct tcggcgtcct
     1921 gagcgtgtac ggctccaccg tcggccacac catcattgac ctgatgtcga agaactgctg
     1981 gggtctgctc ggccactacc tgcgcgtgct gatccacgag catatcctca tccacggcga
     2041 cattcgcaag accaccaaat tgaacattgg tggcactgag attgaggtcg agacgctggt
     2101 ggaggacgag gccgaggctg gcgcggtacc catggtgagc aagggcgagg agctgttcac
     2161 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     2221 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     2281 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca
     2341 gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     2401 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     2461 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     2521 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     2581 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     2641 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     2701 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     2761 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     2821 cactctcggc atggacgagc tgtacaagtg ataagaattc gatatcaagc ttatcgataa
     2881 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2941 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3001 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3061 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3121 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3181 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3241 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3301 ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3361 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3421 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc
     3481 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3541 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3601 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3661 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3721 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     3781 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     3841 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     3901 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     3961 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac
     4021 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
     4081 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
     4141 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     4201 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
     4261 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
     4321 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4381 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4441 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4501 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4561 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
     4621 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4681 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4741 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     4801 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     4861 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4921 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4981 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5041 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5101 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5161 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5221 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5281 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5341 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5401 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5461 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5521 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5581 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5641 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5701 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5761 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5821 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5881 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5941 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6001 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6421 aacggggggt tcgtgcacac a
//
