LOCUS       sequence-256940-        6361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 116_pAAV-ProC24-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        274..470
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      321..456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      477..486
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             483..1409
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1413..1415,1416..1418,1419..2132)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1413..1415,1416..1418,1419..2129)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1458..1479)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1719..1738)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2066..2087
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2160..2748
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2213..2233)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2631..2642)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2780..3256
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2979..2998)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3296..3436
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3296..3425
                     /label=AAV2 ITR
     rep_origin      3511..3966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3598..3617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3808..3829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3983..4002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4102..4124
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4162..4180)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4248..4352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4353..4421,4422..5213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4571..4590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5384..5972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5873..5892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   6034..6163
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 tcactcaaaa acaaaagagg tcggggggta aaacaaagtg aatgatttcg caaaacaagg
       61 ttcgtgtctg cgcaaaacaa tttgagacat aattcaaaac aagacacggg tgaatgaaaa
      121 acaaaaattc caacctggga aaacaaccca tgcaatattt aaaaacaagc caattgctgg
      181 tttaaaacaa ggggtgggta gcccgaaaac aagcaatgga cgttttaaaa acaatgcctg
      241 ccgctgacaa aaacaagctc gagatctgcg atctgcatct caattagtca gcaaccatag
      301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      361 cccatcgctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
      421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa ggatccgcca
      481 ccatggatta tggaggcgcc ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc
      541 cagtagtcgt caatggctct gtacttgtgc ctgaggacca gtgttactgc gcgggctgga
      601 ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg
      661 ctggcttctc catcctactg cttatgtttt acgcctacca aacatggaag tcaacctgcg
      721 gctgggagga gatctatgtg tgcgctatcg agatggtcaa ggtgattctc gagttcttct
      781 tcgagtttaa gaacccgtcc atgctgtatc tagccacagg ccaccgcgtc cagtggttgc
      841 gttacgccga gtggcttctc acctgcccgg tcatttgcat tcacctgtca aacctgacgg
      901 gcttgtccaa cgactacagc aggcgcacca tgggtctgct tgtgtctgat attggcacaa
      961 ttgtgtgggg cgccacttcc gccatggcca ccggatacgt caaggtcatc ttcttctgcc
     1021 tgggtctgtg ttatggtgct aacacgttct ttcacgctgc caaggcctac atcgagggtt
     1081 accacaccgt gccgaagggc cggtgtcgcc aggtggtgac tggcatggct tggctcttct
     1141 tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc
     1201 tgagcgtgta cggctccacc gtcggccaca ccatcattga cctgatgtcg aagaactgct
     1261 ggggtctgct cggccactac ctgcgcgtgc tgatccacga gcatatcctc atccacggcg
     1321 acattcgcaa gaccaccaaa ttgaacattg gtggcactga gattgaggtc gagacgctgg
     1381 tggaggacga ggccgaggct ggcgcggtac ccatggtgag caagggcgag gagctgttca
     1441 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     1501 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     1561 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     1621 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     1681 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1741 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1801 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1861 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1921 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1981 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2041 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     2101 tcactctcgg catggacgag ctgtacaagt gataagaatt cgatatcaag cttatcgata
     2161 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2221 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2281 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2341 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2401 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2461 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2521 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     2581 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2641 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2701 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     2761 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     2821 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     2881 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     2941 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3001 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3061 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     3121 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     3181 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     3241 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     3301 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     3361 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     3421 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3481 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     3541 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     3601 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3661 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3721 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3781 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3841 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     3901 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     3961 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4021 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4081 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     4141 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     4201 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4261 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4321 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4381 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4441 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4501 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4561 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4621 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4681 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4741 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4801 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4861 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4921 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4981 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5041 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     5101 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     5161 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     5221 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5281 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5341 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5401 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5461 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5521 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5581 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5641 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5701 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5761 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5821 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5881 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5941 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6001 tcctggcctt ttgctggcct tttgctcaca tgtcctgcag gcagctgcgc gctcgctcgc
     6061 tcactgaggc cgcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     6121 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg cacgcgtaat
     6181 cgggaacacc taaaaacaaa tttgaagggc acacaaaaca aatgccagac ccctcaaaaa
     6241 caagtcacgt aacgggctaa aacaaccatg tagatgtgtt aaaacaacgg tgctgtaggc
     6301 ccaaaacaaa ctcaactcac gatgaaaaca aatccaacgg ctggtcaaaa caatcacggg
     6361 a
//
