LOCUS       sequence-256946-        9401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for expression of KSHV ORF24 with a C-terminal 
            3xFLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Didychuk AL, Castaneda AF, Kushnir LO, Huang CJ, Glaunsinger BA
  TITLE     Conserved CxnC motifs in Kaposi's sarcoma-associated herpesvirus 
            ORF66 are required for viral late gene expression and are essential 
            for its interaction with ORF34.
  JOURNAL   J Virol. 2019 Oct 2. pii: JVI.01299-19. doi: 10.1128/JVI.01299-19.
  PUBMED    31578296
REFERENCE   2  (bases 1 to 9401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Oct 2. pii: JVI.01299-19. doi: 10.1128/JVI.01299-19."
COMMENT     Sequence Label: pLJM1-zeo-ORF24-3xFLAG
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2660..2725
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    2778..2895
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2944..3454
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3373..3391
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3463..3837
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             3909..4142
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4214..4335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4251..4270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4305..4324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4375..4510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4530..4549)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4531..4549)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4559..4576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4559..4575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4568..4590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4717..5172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4804..4823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5014..5035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5198..5302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5303..5371,5372..6163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5521..5540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6334..6922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6823..6842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7076..7093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7210..7231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7246..7251,7252..7269,7270..7276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7284..7300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7289..7311
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7308..7324
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7308..7324
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7343..7363
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7345..7363
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7391..7617
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7618..7798
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7845..7970
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8463..8696
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8881..8925
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     9223..9243
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg atggcagcgc tcgagggccc cctactactg ccaccgagcg cctccctgac
      421 gacgagtccg cagaccacgt gttatcaagc gacttgggaa tcacagctgg aaatattctg
      481 ctgtctggcc accaactcgc acctgcaggc agagctgacc ttagaaggtc ttgataagat
      541 gatgcagccc gagcccacct ttttcgcctg cagagcgata cgcagactac tcctggggga
      601 acgcctccac ccttttatac atcaagaagg gactcttttg ggaaaagtgg gtcgacggta
      661 cagcggcgaa ggtttaataa ttgacggtgg tggagtgttt acgcgcggac agatagacac
      721 cgacaactac ctacctgcgg tgggatcatg ggaacttacc gatgattgtg ataaaccctg
      781 cgaattcagg gagctacgct cgctgtatct tcccgcgcta ctaacgtgca ccatatgtta
      841 caaagccatg ttcaggatag tgtgcaggta cctggagttc tgggagttcg aacagtgttt
      901 tcatgcgttt ctggcggtgt tgccccatag tctacaaccc acaatctatc aaaattattt
      961 tgcactcctg gagagcctga agcatctctc gttttcaata atgccacccg catccccaga
     1021 cgcacagcta cattttttaa agtttaacat cggcagcttc atggccacgt gggggtggca
     1081 cggagagctg gtctcgctgc gccgtgccat cgctcacaac gtagagcgac tgcccaccgt
     1141 gctgaagaac ctgtcgaaac agagtaagca ccaggacgtc aaggttaacg gacgggatct
     1201 ggtgggcttt cagctggctc taaaccagct cgtgtcccgt ctgcacgtaa aaatccaacg
     1261 caaggacccc ggaccaaagc catacagggt ggtcgtcagt accccagatt gtacctacta
     1321 tctagtgtat ccgggcacac cggccatcta cagactcgtc atgtgtatgg cagtggcaga
     1381 ctgcatcggc cactcgtgca gcggactgca cccctgcgca aactttttag gcacccacga
     1441 gacaccgcgt ctcctggcgg cgacgctttc aagaatccgg tacgcgccga aagaccggcg
     1501 agcagccatg aagggaaatt tgcaggcgtg cttccaacga tacgcggcca cggacgcgcg
     1561 gactctgggc agctccacag tgtcagacat gctggaaccc acaaaacacg tcagtttgga
     1621 aaacttcaag atcaccatat tcaacaccaa catggtgatt aacactaaga taagctgcca
     1681 cgttcctaac accctgcaaa agactatttt aaacatcccc agattgacca acaattttgt
     1741 tatacgaaag tactccgtaa aggaaccttc ttttaccata agcgtgtttt tttccgacaa
     1801 catgtgtcaa ggcaccgcaa taaacatcaa catcagtggg gacatgctgc actttctctt
     1861 cgcaatgggt acgctgaaat gctttctgcc aatcaggcac atatttcctg tatcgatagc
     1921 aaattggaac tccacgttgg acctgcacgg actggaaaac cagtacatgg tgagaatggg
     1981 gcgaaaaaac gtattttgga ccacaaactt tccatctgtg gtctccagca aggatgggct
     2041 aaacgtgtcc tggtttaagg ccgcgacagc cacgatttct aaagtgtacg ggcagcctct
     2101 tgtggaacag attcgccacg agctggcgcc cattctcacg gaccagcacg cgcgcatcga
     2161 cggaaacaaa aatagaatat tctccctact tgagcacaga aaccgttccc aaatacagac
     2221 gctacacaaa aggttcctgg agtgtctggt ggaatgctgt tcgtttctca ggcttgacgt
     2281 ggcttgcatt aggcgagccg ccgcccgggg cctgtttgac ttctcaaaga agataatcag
     2341 tcacactaaa agcaaacacg agtgcgcagt actgggatat aaaaagtgta acctaatccc
     2401 gaaaatctat gcccgaaaca agaagaccag gctagacgag ttgggccgca atgcaaactt
     2461 catttcgttc gtcgccacca cgggtcatcg gttcgccgct ctaaagccac aaattgtccg
     2521 tcacgccatt cgcaaactag gcctgcactg gcgccaccga acggccgcgt ccaacgagca
     2581 gacaccgcca gccgatcccc gcgtacgttg cgtccgtccg ctggtctcag ggcggccgct
     2641 cgagggaggc ggtggagccg actacaagga ccacgacggc gactacaagg accacgacat
     2701 cgactacaag gacgacgacg acaaggggcc cgtttaaaat tctcgacctc gagacaaatg
     2761 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2821 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2881 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gccgcggctc
     2941 gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     3001 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     3061 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     3121 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     3181 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     3241 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     3301 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     3361 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     3421 cctcgttgac cgaatcaccg acctctctcc ccagggggat ccatggccaa gttgaccagt
     3481 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     3541 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     3601 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     3661 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     3721 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     3781 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaggt
     3841 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3901 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     3961 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4141 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     4201 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4321 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     4381 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4441 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4501 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4561 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4621 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4681 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4921 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5041 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5161 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5221 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5281 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5341 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5401 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5461 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5521 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5581 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5641 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5701 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5761 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5821 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5881 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5941 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     6001 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     6061 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     6121 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6181 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6241 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6481 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7081 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7141 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7201 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7261 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7321 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     7381 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     7441 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     7501 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     7561 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     7621 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7681 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7741 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7801 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7861 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7921 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7981 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8041 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8101 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8161 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8221 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8281 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8341 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8401 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8461 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8521 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8581 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8641 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8701 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8761 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8821 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8881 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8941 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9001 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9061 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9121 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     9181 tatcgatcac gagactagcc tcgagcggcc gcccccttca ccgagggcct atttcccatg
     9241 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     9301 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     9361 tagtttgcag ttttaaaatt atgttttaaa atggactatc a
//
