LOCUS       sequence-25699-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Kapust RB, Waugh DS
  TITLE     Controlled intracellular processing of fusion proteins by TEV 
            protease.
  JOURNAL   Protein Expr Purif. 2000 Jul . 19(2):312-8.
  PUBMED    10873547
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2000 Jul . 19(2):312-8."
COMMENT     Sequence Label: pKM586
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     195..213
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(234..252,253..258,259..275,276..281,282..307)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             317..328
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
       61 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
      121 ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt attatcatga
      181 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagtccctat
      241 cagtgataga gattgacatc cctatcagtg atagagatac tgagcacatc agcaggacgc
      301 actgaccgaa ttcattaaag aggagaaagg taccatggga gaaagcttgt ttaaggggcc
      361 gcgtgattac aacccgatat cgagcaccat ttgtcatttg acgaatgaat ctgatgggca
      421 cacaacatcg ttgtatggta ttggatttgg gcccttcatc attacaaaca agcacttgtt
      481 tagaagaaat aatggaacac tgttggtcca atcactacat ggtgtattca aggtcaagaa
      541 caccacgact ttgcaacaac acctcatcga tgggcgggac atgatcatta ttcgaatgcc
      601 taaggatttc ccaccatttc ctcnaaagct gaaatttcgc gagccacaac gggaagagcg
      661 catttgtctt gtgacaacca acttccaaac taagnnnnnn tctagcatgg tgtcagacac
      721 tangttgcac attcccttcn tctgat
//
