LOCUS       sequence-256991-       12516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP tagged mTOR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Jain A, Arauz E, Aggarwal V, Ikon N, Chen J, Ha T
  TITLE     Stoichiometry and assembly of mTOR complexes revealed by 
            single-molecule pulldown.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Dec 16;111(50):17833-8. doi: 
            10.1073/pnas.1419425111. Epub 2014 Dec 1.
  PUBMED    25453101
REFERENCE   2  (bases 1 to 12516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1419425111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-12-16- 16"; volume: "111"; issue: "50"; pages: "17833-8"
COMMENT     Sequence Label: peYFP-C1-mTOR
FEATURES             Location/Qualifiers
     source          1..12516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      623..632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(629..631,632..634,635..1345)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(674..695)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(935..954)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1282..1303
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1379..1388
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4251..4260
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7460..7738
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             7472..7744
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(9159..9175)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9159..9175)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    9320..9441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9357..9376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9411..9430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9448..9903)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9585..9606)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9797..9816
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9930..10034
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10036..10393
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10036..10227
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10059..10079)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10244..10379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10428..11222
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10428..11222
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10482..10501)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11092..11111
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11410..11429)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11454..11501
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11830..12418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12319..12338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
      661 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      721 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      781 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg
      841 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      901 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
      961 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1021 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1081 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     1141 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1201 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaaac tgagcaaaga
     1261 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1321 tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag cttctcgagc
     1381 caccatggac agcggccgca tgcttggaac cggacctgcc gccgccacca ccgctgccac
     1441 cacatctagc aatgtgagcg tcctgcagca gtttgccagt ggcctaaaga gccggaatga
     1501 ggaaaccagg gccaaagccg ccaaggagct ccagcactat gtcaccatgg aactccgaga
     1561 gatgagtcaa gaggagtcta ctcgcttcta tgaccaactg aaccatcaca tttttgaatt
     1621 ggtttccagc tcagatgcca atgagaggaa aggtggcatc ttggccatag ctagcctcat
     1681 aggagtggaa ggtgggaatg ccacccgaat tggcagattt gccaactatc ttcggaacct
     1741 cctcccctcc aatgacccag ttgtcatgga aatggcatcc aaggccattg gccgtcttgc
     1801 catggcaggg gacactttta ccgctgagta cgtggaattt gaggtgaagc gagccctgga
     1861 atggctgggt gctgaccgca atgagggccg gagacatgca gctgtcctgg ttctccgtga
     1921 gctggccatc agcgtcccta ccttcttctt ccagcaagtg caacccttct ttgacaacat
     1981 ttttgtggcc gtgtgggacc ccaaacaggc catccgtgag ggagctgtag ccgcccttcg
     2041 tgcctgtctg attctcacaa cccagcgtga gccgaaggag atgcagaagc ctcagtggta
     2101 caggcacaca tttgaagaag cagagaaggg atttgatgag accttggcca aagagaaggg
     2161 catgaatcgg gatgatcgga tccatggagc cttgttgatc cttaacgagc tggtccgaat
     2221 cagcagcatg gagggagagc gtctgagaga agaaatggaa gaaatcacac agcagcagct
     2281 ggtacacgac aagtactgca aagatctcat gggcttcgga acaaaacctc gtcacattac
     2341 ccccttcacc agtttccagg ctgtacagcc ccagcagtca aatgccttgg tggggctgct
     2401 ggggtacagc tctcaccaag gcctcatggg atttgggacc tcccccagtc cagctaagtc
     2461 caccctggtg gagagccggt gttgcagaga cttgatggag gagaaatttg atcaggtgtg
     2521 ccagtgggtg ctgaaatgca ggaatagcaa gaactcgctg atccaaatga caatccttaa
     2581 tttgttgccc cgcttggctg cattccgacc ttctgccttc acagataccc agtatctcca
     2641 agataccatg aaccatgtcc taagctgtgt caagaaggag aaggaacgta cagcggcctt
     2701 ccaagccctg gggctacttt ctgtggctgt gaggtctgag tttaaggtct atttgcctcg
     2761 cgtgctggac atcatccgag cggccctgcc cccaaaggac ttcgcccata agaggcagaa
     2821 ggcaatgcag gtggacgcca cagtcttcac ttgcatcagc atgctggctc gagcaatggg
     2881 gccaggcatc cagcaggata tcaaggagct gctggagccc atgctggcag tgggactaag
     2941 ccctgccctc actgcagtgc tctacgacct gagccgtcag attccacagc taaagaagga
     3001 cattcaagat gggctactga aaatgctgtc cctggtcctt atgcacaaac cccttcgcca
     3061 cccaggcatg cccaagggcc tggcccatca gctggcctct cctggcctca cgaccctccc
     3121 tgaggccagc gatgtgggca gcatcactct tgccctccga acgcttggca gctttgaatt
     3181 tgaaggccac tctctgaccc aatttgttcg ccactgtgcg gatcatttcc tgaacagtga
     3241 gcacaaggag atccgcatgg aggctgcccg cacctgctcc cgcctgctca caccctccat
     3301 ccacctcatc agtggccatg ctcatgtggt tagccagacc gcagtgcaag tggtggcaga
     3361 tgtgcttagc aaactgctcg tagttgggat aacagatcct gaccctgaca ttcgctactg
     3421 tgtcttggcg tccctggacg agcgctttga tgcacacctg gcccaggcgg agaacttgca
     3481 ggccttgttt gtggctctga atgaccaggt gtttgagatc cgggagctgg ccatctgcac
     3541 tgtgggccga ctcagtagca tgaaccctgc ctttgtcatg cctttcctgc gcaagatgct
     3601 catccagatt ttgacagagt tggagcacag tgggattgga agaatcaaag agcagagtgc
     3661 ccgcatgctg gggcacctgg tctccaatgc cccccgactc atccgcccct acatggagcc
     3721 tattctgaag gcattaattt tgaaactgaa agatccagac cctgatccaa acccaggtgt
     3781 gatcaataat gtcctggcaa caataggaga attggcacag gttagtggcc tggaaatgag
     3841 gaaatgggtt gatgaacttt ttattatcat catggacatg ctccaggatt cctctttgtt
     3901 ggccaaaagg caggtggctc tgtggaccct gggacagttg gtggccagca ctggctatgt
     3961 agtagagccc tacaggaagt accctacttt gcttgaggtg ctactgaatt ttctgaagac
     4021 tgagcagaac cagggtacac gcagagaggc catccgtgtg ttagggcttt taggggcttt
     4081 ggatccttac aagcacaaag tgaacattgg catgatagac cagtcccggg atgcctctgc
     4141 tgtcagcctg tcagaatcca agtcaagtca ggattcctct gactatagca ctagtgaaat
     4201 gctggtcaac atgggaaact tgcctctgga tgagttctac ccagctgtgt ccatggtggc
     4261 cctgatgcgg atcttccgag accagtcact ctctcatcat cacaccatgg ttgtccaggc
     4321 catcaccttc atcttcaagt ccctgggact caaatgtgtg cagttcctgc cccaggtcat
     4381 gcccacgttc cttaatgtca ttcgagtctg tgatggggcc atccgggaat ttttgttcca
     4441 gcagctggga atgttggtgt cctttgtgaa gagccacatc agaccttata tggatgaaat
     4501 agtcaccctc atgagagaat tctgggtcat gaacacctca attcagagca cgatcattct
     4561 tctcattgag caaattgtgg tagctcttgg gggtgaattt aagctctacc tgccccagct
     4621 gatcccacac atgctgcgtg tcttcatgca tgacaacagc ccaggccgca ttgtctctat
     4681 caagttactg gctgcaatcc agctgtttgg cgccaacctg gatgactacc tgcatttact
     4741 gctgcctcct attgttaagt tgtttgatgc ccctgaagct ccactgccat ctcgaaaggc
     4801 agcgctagag actgtggacc gcctgacgga gtccctggat ttcactgact atgcctcccg
     4861 gatcattcac cctattgttc gaacactgga ccagagccca gaactgcgct ccacagccat
     4921 ggacacgctg tcttcacttg tttttcagct ggggaagaag taccaaattt tcattccaat
     4981 ggtgaataaa gttctggtgc gacaccgaat caatcatcag cgctatgatg tgctcatctg
     5041 cagaattgtc aagggataca cacttgctga tgaagaggag gatcctttga tttaccagca
     5101 tcggatgctt aggagtggcc aaggggatgc attggctagt ggaccagtgg aaacaggacc
     5161 catgaagaaa ctgcacgtca gcaccatcaa cctccaaaag gcctggggcg ctgccaggag
     5221 ggtctccaaa gatgactggc tggaatggct gagacggctg agcctggagc tgctgaagga
     5281 ctcatcatcg ccctccctgc gctcctgctg ggccctggca caggcctaca acccgatggc
     5341 cagggatctc ttcaatgctg catttgtgtc ctgctggtct gaactgaatg aagatcaaca
     5401 ggatgagctc atcagaagca tcgagttggc cctcacctca caagacatcg ctgaagtcac
     5461 acagaccctc ttaaacttgg ctgaattcat ggaacacagt gacaagggcc ccctgccact
     5521 gagagatgac aatggcattg ttctgctggg tgagagagct gccaagtgcc gagcatatgc
     5581 caaagcacta cactacaaag aactggagtt ccagaaaggc cccacccctg ccattctaga
     5641 atctctcatc agcattaata ataagctaca gcagccggag gcagcggccg gagtgttaga
     5701 atatgccatg aaacactttg gagagctgga gatccaggct acctggtatg agaaactgca
     5761 cgagtgggag gatgcccttg tggcctatga caagaaaatg gacaccaaca aggacgaccc
     5821 agagctgatg ctgggccgca tgcgctgcct cgaggccttg ggggaatggg gtcaactcca
     5881 ccagcagtgc tgtgaaaagt ggaccctggt taatgatgag acccaagcca agatggcccg
     5941 gatggctgct gcagctgcat ggggtttagg tcagtgggac agcatggaag aatacacctg
     6001 tatgatccct cgggacaccc atgatggggc attttataga gctgtgctgg cactgcatca
     6061 ggacctcttc tccttggcac aacagtgcat tgacaaggcc agggacctgc tggatgctga
     6121 attaactgca atggcaggag agagttacag tcgggcatat ggggccatgg tttcttgcca
     6181 catgctgtcc gagctggagg aggttatcca gtacaaactt gtccccgagc gacgagagat
     6241 catccgccag atctggtggg agagactgca gggctgccag cgtatcgtag aggactggca
     6301 gaaaatcctt atggtgcggt cccttgtggt cagccctcat gaagacatga gaacctggct
     6361 caagtatgca agcctgtgcg gcaagagtgg caggctggct cttgctcata aaactttagt
     6421 gttgctcctg ggagttgatc cgtctcggca acttgaccat cctctgccaa cagttcaccc
     6481 tcaggtgacc tatgcctaca tgaaaaacat gtggaagagt gcccgcaaga tcgatgcctt
     6541 ccagcacatg cagcattttg tccagaccat gcagcaacag gcccagcatg ccatcgctac
     6601 tgaggaccag cagcataagc aggaactgca caagctcatg gcccgatgct tcctgaaact
     6661 tggagagtgg cagctgaatc tacagggcat caatgagagc acaatcccca aagtgctgca
     6721 gtactacagc gccgccacag agcacgaccg cagctggtac aaggcctggc atgcgtgggc
     6781 agtgatgaac ttcgaagctg tgctacacta caaacatcag aaccaagccc gcgatgagaa
     6841 gaagaaactg cgtcatgcca gcggggccaa catcaccaac gccaccactg ccgccaccac
     6901 ggccgccact gccaccacca ctgccagcac cgagggcagc aacagtgaga gcgaggccga
     6961 gagcaccgag aacagcccca ccccatcgcc gctgcagaag aaggtcactg aggatctgtc
     7021 caaaaccctc ctgatgtaca cggtgcctgc cgtccagggc ttcttccgtt ccatctcctt
     7081 gtcacgaggc aacaacctcc aggatacact cagagttctc accttatggt ttgattatgg
     7141 tcactggcca gatgtcaatg aggccttagt ggagggggtg aaagccatcc agattgatac
     7201 ctggctacag gttatacctc agctcattgc aagaattgat acgcccagac ccttggtggg
     7261 acgtctcatt caccagcttc tcacagacat tggtcggtac cacccccagg ccctcatcta
     7321 cccactgaca gtggcttcta agtctaccac gacagcccgg cacaatgcag ccaacaagat
     7381 tctgaagaac atgtgtgagc acagcaacac cctggtccag caggccatga tggtgagcga
     7441 ggagctgatc cgagtggcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc
     7501 atctcgtttg tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt
     7561 gcatgctatg atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta
     7621 tggtcgagat ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt
     7681 caaggacctc acccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaagca
     7741 gctgcctcag ctcacatcct tagagctgca atatgtttcc ccaaaacttc tgatgtgccg
     7801 ggaccttgaa ttggctgtgc caggaacata tgaccccaac cagccaatca ttcgcattca
     7861 gtccatagca ccgtctttgc aagtcatcac atccaagcag aggccccgga aattgacact
     7921 tatgggcagc aacggacatg agtttgtttt ccttctaaaa ggccatgaag atctgcgcca
     7981 ggatgagcgt gtgatgcagc tcttcggcct ggttaacacc cttctggcca atgacccaac
     8041 atctcttcgg aaaaacctca gcatccagag atacgctgtc atccctttat cgaccaactc
     8101 gggcctcatt ggctgggttc cccactgtga cacactgcac gccctcatcc gggactacag
     8161 ggagaagaag aagatccttc tcaacatcga gcatcgcatc atgttgcgga tggctccgga
     8221 ctatgaccac ttgactctga tgcagaaggt ggaggtgttt gagcatgccg tcaataatac
     8281 agctggggac gacctggcca agctgctgtg gctgaaaagc cccagctccg aggtgtggtt
     8341 tgaccgaaga accaattata cccgttcttt agcggtcatg tcaatggttg ggtatatttt
     8401 aggcctggga gatagacacc catccaacct gatgctggac cgtctgagtg ggaagatcct
     8461 gcacattgac tttggggact gctttgaggt tgctatgacc cgagagaagt ttccagagaa
     8521 gattccattt agactaacaa gaatgttgac caatgctatg gaggttacag gcctggatgg
     8581 caactacaga atcacatgcc acacagtgat ggaggtgctg cgagagcaca aggacagtgt
     8641 catggccgtg ctggaagcct ttgtctatga ccccttgctg aactggaggc tgatggacac
     8701 aaataccaaa ggcaacaagc gatcccgaac gaggacggat tcctactctg ctggccagtc
     8761 agtcgaaatt ttggacggtg tggaacttgg agagccagcc cataagaaaa cggggaccac
     8821 agtgccagaa tctattcatt ctttcattgg agacggtttg gtgaaaccag aggccctaaa
     8881 taagaaagct atccagatta ttaacagggt tcgagataag ctcactggtc gggacttctc
     8941 tcatgatgac actttggatg ttccaacgca agttgagctg ctcatcaaac aagcgacatc
     9001 ccatgaaaac ctctgccagt gctatattgg ctggtgccct ttctggtaac tggaggccca
     9061 gatgtgccca tcacgttttt tctgaggctt ttgtacttta gtaaatgctt ccactaaact
     9121 gaaaccatgg tgagaaagtt tgactttgtt aaatggggga tccactagtt ctagagcggc
     9181 cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata ccacatttgt
     9241 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     9301 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     9361 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9421 caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     9481 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     9541 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     9601 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     9661 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     9721 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     9781 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     9841 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     9901 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9961 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10021 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
    10081 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    10141 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    10201 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10261 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10321 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10381 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
    10441 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
    10501 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
    10561 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
    10621 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
    10681 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
    10741 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
    10801 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
    10861 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
    10921 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
    10981 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
    11041 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
    11101 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
    11161 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
    11221 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
    11281 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
    11341 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
    11401 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
    11461 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
    11521 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
    11581 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
    11641 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
    11701 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    11761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    11821 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    11881 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    11941 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    12001 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    12061 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    12121 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    12181 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    12241 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12301 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12361 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12421 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12481 ctttcctgcg ttatcccctg attctgtgga taaccg
//
