LOCUS       sequence-256994-       10708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 E439delE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-E439delE
FEATURES             Location/Qualifiers
     source          1..10708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..60
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(242..283)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    336..360
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2528..2554)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(2560..2584)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(2604..3782)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2613..3551
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(2636..2656)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3830..3947
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4240..4284)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4469..4702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5199..5324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5371..6004
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6196..6215)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6265..6281)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6265..6281)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6278..6300)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6289..6305
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6313..6319,6320..6337,6338..6343))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6358..6379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6619..6636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6790..7378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6870..6889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7549..8340,8341..8409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8172..8191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8410..8514)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8582..8600
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             8719..8952
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    9027..9615
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9133..9144
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9542..9562
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(9629..10342,10343..10345,10346..10348))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9632..10342,10343..10345,10346..10348))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9674..9695)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10023..10042
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10282..10303
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10360..10708,1..204)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(10362..10708,1..221)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10522..10541)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag
       61 gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat
      121 tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt
      181 ccaagcggct tcggccagta acgttagggg ggggggaggg atctcattac taaccggtac
      241 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
      301 ctagactcga gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagttgggc
      361 atcgcctctg ctgtgcatat actgggtagg ctagtgtgac attgagggag tcgttgtgct
      421 gcacaaggga ggtgtgttgg taatgtagca ccagttcttt cagagagctg tacaagttat
      481 agggctcggc aaagccatag ccagttgctg ttttgtttat gacacaatgc tttacttcgc
      541 cgtccaccac tacagagcag gcatagcagc cctgtttact gctctcccgg acaagaaaag
      601 tgccatctcg cttccctcgc aacaggtttt cagctttgtt tcggttgctg cttccaacat
      661 tccatgtctt ctcatcatga tggggcaaat cttcatcatc ttccaccagt gaatattggt
      721 cttcagtgtt ttcattgccc aaccactcgt tcaacttctt ttgccgaaca cctttttgag
      781 tcaaccacat caagtattgg tctctcgtct ttctcagctg gataaggtct ggtttaatgc
      841 tgttcatacg tttgtcaatt tctcgatact cagctgcctg cttcttcaag tcttcttcca
      901 atcttcttct actgtcaata atttcactga ttcgagactt caacttatca taattatgca
      961 taatcctttg tatttctttc tcattgcctt cacgtttaaa cttttctatg tattctttgc
     1021 tgtaccgctc ttgggtctgg cactgttctt caaatatttt tatggtttca ttaaatgctt
     1081 caatagctgt ccttttcatt tggatttcct gggatgtgcg ggtatattct tcatataatc
     1141 tatcatattc tcgacttttt tcttgaaact gagtgttata ttcatgtaat tttttcccta
     1201 cagcttcaat attatctttg acaacttgat cctgttggta tttggatact ggataaagta
     1261 atttcacatc caatttggga ttatactgag ctagagattc attccggtag tggtttatta
     1321 attcaaccac agaactgaag gttaatgggt cagagaagcc atatttccca tctcgatgaa
     1381 atattttgat taatttgtta tttccccctt tccttagtgt aagagtataa tcaccatgca
     1441 ttttagtaga cgcatctcgt accaaaaagg tcccgtctgc tgtatctcga agtttttcat
     1501 tcacttcttc cctcgagata tctccccagt accattcagc atcttgtaag gacatattgt
     1561 tattcatacc gttgttggct acagtagtag gttttggtgg tttaggaggc agtgctggtg
     1621 caggctgtcg ttcattccat tcagttgaga ttaaaatttc tataactttt atgaggtttt
     1681 cagtattatc agagctggct gctgagaatc tgaaaagcat agggctgaaa atttcagaga
     1741 gtactcttgc attcaacaga tttttgctgg aggtttgaga gagcttgaag aaatgtttta
     1801 acaaatactg aagcgtaagc caatactgat gaggtatgct aggcgaccta ataagcttct
     1861 tcaatagctg aatatattct tcggagcttt gtacttctgg agctaaagaa atcatttcac
     1921 tgtaaacggc tgctggaatg acaggatttg gtaagtccag gagatagcgt ttgaaagcgt
     1981 cagccaaaac gtgcacatcg atcatttcca agtccacgga gggtgtatca caatcaagaa
     2041 gctgtcgtaa ttctgccagg ttgctggagc tctgtgttct gtatagagtt gaacattcca
     2101 gacctttctt ttcaatggct tccacgagct tgataagaag aggcggggca atgtcaggag
     2161 gggcaaactg ctctgcaaga tccgggagag tcaaagcttg ttgttcaaca tctgcttcag
     2221 ttttcgaaga acctggtgca acaggaagag gccgaggtgg ccggggcttt ggtgtgggag
     2281 gcgagatttt tttccttcca atatattcta cataagttcc cggaaagtcc cccctttccc
     2341 ctgtggtttc attatagcca tttaaccagc caatttcttc aggcctggct tcctgtccat
     2401 cactgaatcc aagagctact aaggaccctt tattcacagt caatatgtca cccaagtgca
     2461 agtcaatatc ttcttctctt tcctttttat aatcatacag cgctctgtac tggtacccct
     2521 cagcactagc gtaatctgga acatcgtatg ggtaaaccat gccaactttt ttgtacaaac
     2581 ttgttgatat ctgcagggcc gcttcacgac acctgaaatg gaagaaaaaa actttgaacc
     2641 actgtctgag gcttgagaat gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag
     2701 aattacatca agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac
     2761 tccatcgcat aaaacccctc cccccaacct aaagacgacg tactccaaaa gctcgagaac
     2821 taatcgaggt gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag
     2881 gacggaaagg cccttttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc
     2941 gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg gccatctttc cgctcacgca
     3001 actggtgccg accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg
     3061 ccaggcacca gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc
     3121 gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc
     3181 gcggccccaa aaaccgaaat accagtgtgc agatcttggc ccgcatttac aagactatct
     3241 tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg
     3301 aaagcagcga gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca
     3361 ggccaggcct caactcaagc acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc
     3421 tcccacccac ttccaacccg aagctcggga tcaagaatca cgtactgcag ccaggtggaa
     3481 gtaattcaag gcacgcaagg gccataaccc gtaaagaggc caggcccgcg ggaaccacac
     3541 acggcactta cctgtgttct ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact
     3601 actgcactta tatacggttc tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca
     3661 tcactttccc agtttacccc gcgccacctt ctctaggcac cggttcaatt gccgacccct
     3721 ccccccaact tctcggggac tgtgggcgat gtgcgctctg cccactgacg ggcaccggag
     3781 cctcacgact agtccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
     3841 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
     3901 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
     3961 ctgccatttg tgaattcggc gataccgtcg agatccgttc actaatcgaa tggatctgtc
     4021 tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc ccctcggggt
     4081 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     4141 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     4201 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     4261 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     4321 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     4381 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     4441 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     4501 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     4561 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     4621 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     4681 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
     4741 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
     4801 ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca gcggccggcc
     4861 gcttgctgtg cggtggtctt acttttgttt tgctcttcct ctatcttgtc taaagcttcc
     4921 ttggtgtctt ttatctctat cctttgatgc acacaataga gggttgctac tgtattatat
     4981 aatgatctaa gttcttctga tcctgtctga agggatggtt gtagctgtcc cagtatttgt
     5041 ctacagcctt ctgatgtttc taacaggcca ggattaactg cgaatcgttc tagctccctg
     5101 cttgcccata ctatatgttt taatttatat tttttctttc cccctggcct taaccgaatt
     5161 ttttcccatc gcgatctaat tctcccccgc ttaatactga cgctctcgca cccatctctc
     5221 tccttctagc ctccgctagt caaaattttt ggcgtactca ccagtcgccg cccctcgcct
     5281 cttgccgtgc gcgcttcagc aagccgagtc ctgcgtcgag agagctcctc tggtttccct
     5341 ttcgctttca agtccctgtt cgggcgccac tgctagagat tttccacact gactaaaagg
     5401 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     5461 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     5521 aggctcagat ctggtctaac cagagagacc cagtacaggc aaaaagcagc tgcttatatg
     5581 caggatctga gggctcgcca ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc
     5641 ccagcggaaa gtcccttgta gcaagctcga tatcagcagt tcttgaagta ctccggatgc
     5701 agctctcggg ccacgtgatg aaatgctagg cggctgtcaa acctccactc taacacttct
     5761 ctctccgggt catccatccc atgcaggctc acagggtgta acaagctggt gttctctcct
     5821 ttattggcct cttctacctt atctggctca actggtacta gcttgtagca ccatccaaag
     5881 gtcagtggat atctgacccc tggccctggt gtgtagttct gctaatcagg gaagtagcct
     5941 tgtgtgtggt agatccacag atcaaggata tcttgtcttc tttgggagtg aattagccct
     6001 tccaactact aagtttgtag tacatattta acaaatacaa tttctttaaa atgaaaataa
     6061 ttcagaggaa tcacaggttt agagtaaatg aaaccacagg taattggcag tggtaatagg
     6121 gtatggggtg ggaagtttgg gatgattttg gttagcttga gttatccagt tgatccagac
     6181 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     6241 tttatttgtg aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     6301 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6361 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6421 gtcgtgccac ggggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     6481 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     6541 agccatgagc ttgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7201 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7501 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7561 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7621 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7681 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7741 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7801 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7861 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7921 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7981 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8041 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8101 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8161 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8221 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8281 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8341 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8401 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8461 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8521 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8581 aaataggcgt atcacgaggc cctttcgtct tcaagaatga tctagccctt tccttaatta
     8641 acccgggcct ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa
     8701 gatgacatga actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     8761 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     8821 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     8881 ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa
     8941 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     9001 aagtcattgg tcttaaagga tctcaggcgg ggaggcggcc caaagggaga tccgactcgt
     9061 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg
     9121 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
     9181 ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga
     9241 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
     9301 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
     9361 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
     9421 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     9481 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     9541 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     9601 atccagaggt tgattaggat ctatcgattt acttgtacag ctcgtccatg ccgagagtga
     9661 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     9721 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     9781 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     9841 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     9901 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     9961 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
    10021 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
    10081 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
    10141 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
    10201 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
    10261 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
    10321 tgaacagctc ctcgcccttg ctcaccatat gtgtggccat attatcatcg tgtttttcaa
    10381 aggaaaacca cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca
    10441 catgtaaagc atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg
    10501 gcatccttca gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact
    10561 atccaactca caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt
    10621 ggcttttggc cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc
    10681 gctacagacg ttgtttgtct tcaagaag
//
