LOCUS       sequence-257004-        7716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7716)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 3_pAAV-ProA8-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2160..2169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2166..3092
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3096..3098,3099..3101,3102..3815)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3096..3098,3099..3101,3102..3812)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3141..3162)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3402..3421)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3749..3770
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3843..4431
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3896..3916)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4314..4325)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4463..4939
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4662..4681)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4979..5119
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4979..5108
                     /label=AAV2 ITR
     rep_origin      5194..5649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5281..5300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5491..5512
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5666..5685)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5785..5807
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5845..5863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5931..6035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6036..6104,6105..6896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6254..6273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7067..7655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7556..7575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgccagggt ctcaccctgt aactttactt ttttttttta
      181 agatttattt attattatag gtaagtatac tgtagctgtc tgaagacact ccagaagagg
      241 gcatcagatt tagttatgga tggttgtgag ccaccatgtg gctgctggga tttgaactcg
      301 ggaccctcag aagagcagtc ggcgctctta accactgagc catctcacca gccccttacc
      361 ctgtaacttt aggctagctt tgaactctat cctcctgcct cagctccccc agtgatgaaa
      421 ttatcagtat gagccaccac actgaaactg ttggcatatt cttgaaacac tttaccctag
      481 agttttccta gggaggaccc aggtttaaac tctccccgcc cccaccccac ccccaagaca
      541 gggtttctct gtgtatctct ggctgtcctg gaactcactc tgtagaccag gctggcctca
      601 aacctctacc tctccaggct aggattaaag gcttgcaaca ccaccaccca ggttaaaaag
      661 atgtttttgt ttgtttgttt gtttgtttgt tttttcaaat cagggtttct ctgtgtatct
      721 ctggctgtct tcgaactcac tctgtagacc aggctggtct ggaactcaga gatctgcctg
      781 cctctgcctc ctgagtgcta ggattaaatg tgtgagccac catctcccat aaactaactc
      841 cttctattag cttgttcttt gtgctttctt ttcggatcca ccaataggat acactttctg
      901 acaactttta aaatctgata tgccttcact tcgaatcaaa gcccacttgg tgggttcaga
      961 tgaattgtct cttgtcactc ttcacaaaac aagaggagga caggcacatg gaagcagatc
     1021 attttagcca aatgtccatc gtccatcaaa gtccgtcttg aaacacgggt ggtagcactg
     1081 aggtccagta aagcagaacc aataacttca tgctaattac acaagtaccc agggttctgc
     1141 gcagcactca cctgactggc acacagctgc ccacgcaagg agatgtccac ccctcctccc
     1201 tgaccccggg aagcacaact agcttgtcat aaacatcact ttgcacagac ggttccattc
     1261 aagtttgaaa caagcccccc cccccccaaa gctaatgaga tctgtcatat tgatacatct
     1321 tgaccaggaa tcccgaacat ctcccccact ttccatcttc ctctccacaa tccagctagt
     1381 ctccgccaaa gttgtggaga gcgcctagaa gcctctcgct ctcactcccc ggcgattaag
     1441 ttggttcctc aaattgttcc ggacaggagg ggcaggaaac agcgccggct ttggtgacgg
     1501 ctgcagcgga gtgggtgagg gctgtccaag cggcctcaca tcgatccctg gtttaaggtc
     1561 tggttaggtc acaattctag cctccctccc ccaaccgcct gtagtcctgg agcggccacg
     1621 tccctagagg cctgagtttc cttaagaggc cctgctgcta gtccagccag gtgggaggaa
     1681 gctggtccaa gtctggtggc cctaggtggg cggagcctaa ttgtggctcg agctagaagg
     1741 agtgtgacca ggaggtgccg ctcaccctta gcgatccggt acgcgtccct gcacccccga
     1801 gagctctcag tccctgggag acaggtgctg tggccgcgcg gcggcgcccc cgaggggagg
     1861 tcctggagtg cccgccctca cccgcccagt ctacgcccct cctcttctct cccctccccc
     1921 gcagtccact gccaccggtc gccagcgatc ccgttcccgg gtctccgagc agacccagag
     1981 tgcgctcgcc gaggtgttgg gacacttgcg gacctgaccc tggagatcac ccgggtttgc
     2041 ccgcctccca tcatccgcga ttccctggcc cgcgatcctg agcccaggga actcattggg
     2101 tgggggtagg gtcctcgggg acccgggcca gctcccgggg cgacggcggc cgcagatccg
     2161 ccaccatgga ttatggaggc gccctgagtg ccgttgggcg cgagctgcta tttgtaacga
     2221 acccagtagt cgtcaatggc tctgtacttg tgcctgagga ccagtgttac tgcgcgggct
     2281 ggattgagtc gcgtggcaca aacggtgccc aaacggcgtc gaacgtgctg caatggcttg
     2341 ctgctggctt ctccatccta ctgcttatgt tttacgccta ccaaacatgg aagtcaacct
     2401 gcggctggga ggagatctat gtgtgcgcta tcgagatggt caaggtgatt ctcgagttct
     2461 tcttcgagtt taagaacccg tccatgctgt atctagccac aggccaccgc gtccagtggt
     2521 tgcgttacgc cgagtggctt ctcacctgcc cggtcatttg cattcacctg tcaaacctga
     2581 cgggcttgtc caacgactac agcaggcgca ccatgggtct gcttgtgtct gatattggca
     2641 caattgtgtg gggcgccact tccgccatgg ccaccggata cgtcaaggtc atcttcttct
     2701 gcctgggtct gtgttatggt gctaacacgt tctttcacgc tgccaaggcc tacatcgagg
     2761 gttaccacac cgtgccgaag ggccggtgtc gccaggtggt gactggcatg gcttggctct
     2821 tcttcgtatc atggggtatg ttccccatcc tgttcatcct cggccccgag ggcttcggcg
     2881 tcctgagcgt gtacggctcc accgtcggcc acaccatcat tgacctgatg tcgaagaact
     2941 gctggggtct gctcggccac tacctgcgcg tgctgatcca cgagcatatc ctcatccacg
     3001 gcgacattcg caagaccacc aaattgaaca ttggtggcac tgagattgag gtcgagacgc
     3061 tggtggagga cgaggccgag gctggcgcgg tacccatggt gagcaagggc gaggagctgt
     3121 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3181 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3241 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3301 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3361 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3421 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3481 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3541 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3601 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3661 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     3721 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3781 ggatcactct cggcatggac gagctgtaca agtgataaga attcgatatc aagcttatcg
     3841 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3901 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     3961 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4021 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4081 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4141 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4201 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     4261 tcgcctatgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     4321 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     4381 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     4441 gagcgctgct cgagagatct acgggtggca tccctgtgac ccctccccag tgcctctcct
     4501 ggccctggaa gttgccactc cagtgcccac cagccttgtc ctaataaaat taagttgcat
     4561 cattttgtct gactaggtgt ccttctataa tattatgggg tggagggggg tggtatggag
     4621 caaggggcaa gttgggaaga caacctgtag ggcctgcggg gtctattggg aaccaagctg
     4681 gagtgcagtg gcacaatctt ggctcactgc aatctccgcc tcctgggttc aagcgattct
     4741 cctgcctcag cctcccgagt tgttgggatt ccaggcatgc atgaccaggc tcagctaatt
     4801 tttgtttttt tggtagagac ggggtttcac catattggcc aggctggtct ccaactccta
     4861 atctcaggtg atctacccac cttggcctcc caaattgctg ggattacagg cgtgaaccac
     4921 tgctcccttc cctgtccttc tgattttgta ggtaaccacg tgcggaccga gcggccgcag
     4981 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     5041 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     5101 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     5161 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
     5221 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
     5281 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     5341 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5401 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5461 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5521 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
     5581 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     5641 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     5701 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5761 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5821 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     5881 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5941 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6001 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6061 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6121 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6181 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6241 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6301 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6361 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6421 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6481 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6541 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6601 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6661 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6721 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6781 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6841 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6901 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6961 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7021 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7081 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7141 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7201 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7261 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7321 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7381 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7441 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7501 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7561 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7621 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7681 ggttcctggc cttttgctgg ccttttgctc acatgt
//
