LOCUS       sequence-257016-        6933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: I_pAAV-ProA6-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             189..200
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      1377..1386
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1383..2309
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2313..2315,2316..2318,2319..3032)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2313..2315,2316..2318,2319..3029)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2358..2379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2619..2638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2966..2987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3060..3648
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3113..3133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3531..3542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3680..4156
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3879..3898)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4196..4336
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4196..4325
                     /label=AAV2 ITR
     rep_origin      4411..4866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4498..4517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4708..4729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4883..4902)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5002..5024
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5062..5080)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5148..5252
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5253..5321,5322..6113)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5471..5490)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6284..6872
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6773..6792
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctagca cagcactagg ctaaagcgta ctgagccctt
      181 gtcttccgtg ggagctgcag agtgggatgc atgcgttgtg agctgaggct caagctgcgc
      241 tggcagaaga gcaggggttg ccttgtcaga ctccagggtc tctttctctc tgagcctggg
      301 aaagtgccac tttattggat ctataaagcc gggggggggg ggggggagga atctcaaggt
      361 gaagaggaag ttcacagacc cctctaacgc ctctattaga accttccagc tattctctca
      421 tacttgtaca ctgagctggc acacagtata ggcaagttct attcgcatca cccctctagt
      481 tcctgtctcc ctggttatgc aagcctcata tttaggtaga tgtgacctta ggaaaccaaa
      541 atatccttta agatcttact aactggttgc ctgttcagct tttccacatt gatcctgtag
      601 ccccctcgag gaggtgaagg aaaaaaatct cctctttgtt tctctaactc attaatgaat
      661 tttaagggca ctctgtaagg ttcctttccc attctggtct ggttcgtaca ttctgagaaa
      721 cacactgtgt ttgtgttgag agttggctcc ctagctacac tgtctgtcac attgatgctc
      781 tgagtaggga cagggttcat ctaggaaata tattttcact cacactctgt atcttttcct
      841 agtttggcat attctagtct gcatttggct ctctgtttaa atataaaaga aaactaaaac
      901 acacccttca gacgcctatg tctgaaaaat ctggcatttc cgtgggtttt tctttaagga
      961 ggccttcatt tgtaaccaac accatgctct ccttaaggaa atcaatctca atgccctatt
     1021 atccttccct tttctttcct cccagtttga ggctgcagtt gccttttttt ttcttatccc
     1081 ctgctgaacc tgaaaaaccc tctcttttct acagttttct gttcccaggc cccgctgact
     1141 tcctttagag catggggggg ggggggatca ggattgtgat gtgtgaactg ggaggatctt
     1201 gacctactcc gctaacccag tggcctgagc aaatcacaag gaggattgga gccatctgcc
     1261 cagcccctcc cccacggcag cctgctggaa agagacaagt tagtcattca aatgattggc
     1321 tttttgcccg cttcttctct aaataagaag gcagcagctt ctgctgaggt ggatccgcca
     1381 ccatggatta tggaggcgcc ctgagtgccg ttgggcgcga gctgctattt gtaacgaacc
     1441 cagtagtcgt caatggctct gtacttgtgc ctgaggacca gtgttactgc gcgggctgga
     1501 ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa cgtgctgcaa tggcttgctg
     1561 ctggcttctc catcctactg cttatgtttt acgcctacca aacatggaag tcaacctgcg
     1621 gctgggagga gatctatgtg tgcgctatcg agatggtcaa ggtgattctc gagttcttct
     1681 tcgagtttaa gaacccgtcc atgctgtatc tagccacagg ccaccgcgtc cagtggttgc
     1741 gttacgccga gtggcttctc acctgcccgg tcatttgcat tcacctgtca aacctgacgg
     1801 gcttgtccaa cgactacagc aggcgcacca tgggtctgct tgtgtctgat attggcacaa
     1861 ttgtgtgggg cgccacttcc gccatggcca ccggatacgt caaggtcatc ttcttctgcc
     1921 tgggtctgtg ttatggtgct aacacgttct ttcacgctgc caaggcctac atcgagggtt
     1981 accacaccgt gccgaagggc cggtgtcgcc aggtggtgac tggcatggct tggctcttct
     2041 tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg ccccgagggc ttcggcgtcc
     2101 tgagcgtgta cggctccacc gtcggccaca ccatcattga cctgatgtcg aagaactgct
     2161 ggggtctgct cggccactac ctgcgcgtgc tgatccacga gcatatcctc atccacggcg
     2221 acattcgcaa gaccaccaaa ttgaacattg gtggcactga gattgaggtc gagacgctgg
     2281 tggaggacga ggccgaggct ggcgcggtac ccatggtgag caagggcgag gagctgttca
     2341 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     2401 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     2461 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     2521 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     2581 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     2641 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     2701 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     2761 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     2821 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     2881 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     2941 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     3001 tcactctcgg catggacgag ctgtacaagt gataagaatt cgatatcaag cttatcgata
     3061 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3121 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3181 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3241 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3301 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3361 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3421 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3481 cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3541 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3601 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgag
     3661 cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc
     3721 cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat
     3781 tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa
     3841 ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag
     3901 tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct
     3961 gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt
     4021 gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc
     4081 tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc
     4141 tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg gccgcaggaa
     4201 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4261 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4321 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4381 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4741 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     4861 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     4921 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     4981 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5041 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5101 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5161 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5221 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5281 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5341 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5401 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5461 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5521 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5581 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5641 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     5761 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     5821 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     5881 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     5941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6241 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6301 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6361 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6421 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6481 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6541 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6601 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6661 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6721 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     6781 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     6841 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     6901 tcctggcctt ttgctggcct tttgctcaca tgt
//
