LOCUS       sequence-257021-        7804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 28_pAAV-ProA10-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             973..984
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      2248..2257
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2254..3180
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3184..3186,3187..3189,3190..3903)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3184..3186,3187..3189,3190..3900)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3229..3250)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3490..3509)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3837..3858
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3931..4519
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3984..4004)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4402..4413)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4551..5027
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4750..4769)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5067..5207
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5067..5196
                     /label=AAV2 ITR
     rep_origin      5282..5737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5369..5388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5579..5600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5754..5773)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5873..5895
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5933..5951)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6019..6123
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6124..6192,6193..6984)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6342..6361)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7155..7743
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7644..7663
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgttctt tgggttccat attttcctca ttccccttca
      181 agtctagctg gattgcaaag ttaagcttat gattatgaat aaaaactaaa taagaaaaaa
      241 aagtcaagat cagatttaga atttaaaaca actatacaga ggtaccatta actaaattca
      301 atttatatgt tcttaagatg tatacaaaat aaagtttgat atattcgtga aatttttgtt
      361 ttaaccaaga aaagaaaaaa aaaataaaaa ggattttttt gcaaaaacac atatccacca
      421 agtagcttat ctaaaatata caaagaatat gtatatagca cagcagaaag ggaaaaaaaa
      481 accccaactt taaagtagga aaatatttac atagtcactg catgaacggt attcaaaacc
      541 cagaaaatca cattaaaaaa atgttcagtg tccttagtag tgaggggaat gcaaatttgt
      601 gtttgaaagg ttgctggttt ccaagagatc ctttgagaac agtgtggtag ctgctgctaa
      661 agaatcaggc aaagccagaa gttgctcaca tttaggaggc ttgtgtgctg ggggttggga
      721 ggatgactgc taaatactgg ggaagcaaaa tgagctaagt ctttacagca actgtcattt
      781 gtgagagtaa accattagaa acggacggca ggtttgtggt agattccaca aggtggtgga
      841 actagtcgca tatgtagttc agacttgaag gcacacacgt atggggacag ttctggtgtt
      901 tacgataaac aggtatgctg ccatccagct agcacagcac taggctaaag cgtactgagc
      961 ccttgtcttc cgtgggagct gcagagtggg atgcatgcgt tgtgagctga ggctcaagct
     1021 gcgctggcag aagagcaggg gttgccttgt cagactccag ggtctctttc tctctgagcc
     1081 tgggaaagtg ccactttatt ggatctataa agccgggggg gggggggggg grggaatctc
     1141 aaggkgaaga ggaagttcac agacccctct aacgcctcta ttagaacctt ccagctattc
     1201 tctcatactt gtacactgag ctggcacaca gtataggcaa gttctattcg catcacccct
     1261 ctagttcctg tctccctggt tatgcaagcc tcatatttag gtagatgtga ccttaggaaa
     1321 ccaaaatatc ctttaagatc ttactaactg gttgcctgtt cagcttttcc acattgatcc
     1381 tgtagccccc tcgaggaggt gaaggaaaaa aatctcctct ttgtttctct aactcattaa
     1441 tgaattttaa gggcactctg taaggttcct ttcccattct ggtctggttc gtacattctg
     1501 agaaacacac tgtgtttgtg ttgagagttg gctccctagc tacactgtct gtcacattga
     1561 tgctctgagt agggacaggg ttcatctagg aaatatattt tcactcacac tctgtatctt
     1621 ttcctagttt ggcatattct agtctgcatt tggctctctg tttaaatata aaagaaaact
     1681 aaaacacacc cttcagacgc ctatgtctga aaaatctggc atttccgtgg gtttttcttt
     1741 aaggaggcct tcatttgtaa ccaacaccat gctctcctta aggaaatcaa tctcaatgcc
     1801 ctattatcct tcccttttct ttcctcccag tttgaggctg cagttgcctt tttttttctt
     1861 atcccctgct gaacctgaaa aaccctctct tttctacagt tttctgttcc caggccccgc
     1921 tgacttcctt tagagcatgg gggggggggg gatcaggatt gtgatgtgtg aactgggagg
     1981 atcttgacct actccgctaa cccagtggcc tgagcaaatc acaaggagga ttggagccat
     2041 ctgcccagcc cctcccccac ggcagcctgc tggaaagaga caagttagtc attcaaatga
     2101 ttggcttttt gcccgcttct tctctaaata agaaggcagc agcttctgct gaggtgcaaa
     2161 gggtttttgt gaattacggt ggtgggtaag acccgagttc ctccagttcc tgcctatttt
     2221 cagctgacta ggcggttaag gggatccgcc accatggatt atggaggcgc cctgagtgcc
     2281 gttgggcgcg agctgctatt tgtaacgaac ccagtagtcg tcaatggctc tgtacttgtg
     2341 cctgaggacc agtgttactg cgcgggctgg attgagtcgc gtggcacaaa cggtgcccaa
     2401 acggcgtcga acgtgctgca atggcttgct gctggcttct ccatcctact gcttatgttt
     2461 tacgcctacc aaacatggaa gtcaacctgc ggctgggagg agatctatgt gtgcgctatc
     2521 gagatggtca aggtgattct cgagttcttc ttcgagttta agaacccgtc catgctgtat
     2581 ctagccacag gccaccgcgt ccagtggttg cgttacgccg agtggcttct cacctgcccg
     2641 gtcatttgca ttcacctgtc aaacctgacg ggcttgtcca acgactacag caggcgcacc
     2701 atgggtctgc ttgtgtctga tattggcaca attgtgtggg gcgccacttc cgccatggcc
     2761 accggatacg tcaaggtcat cttcttctgc ctgggtctgt gttatggtgc taacacgttc
     2821 tttcacgctg ccaaggccta catcgagggt taccacaccg tgccgaaggg ccggtgtcgc
     2881 caggtggtga ctggcatggc ttggctcttc ttcgtatcat ggggtatgtt ccccatcctg
     2941 ttcatcctcg gccccgaggg cttcggcgtc ctgagcgtgt acggctccac cgtcggccac
     3001 accatcattg acctgatgtc gaagaactgc tggggtctgc tcggccacta cctgcgcgtg
     3061 ctgatccacg agcatatcct catccacggc gacattcgca agaccaccaa attgaacatt
     3121 ggtggcactg agattgaggt cgagacgctg gtggaggacg aggccgaggc tggcgcggta
     3181 cccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     3241 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     3301 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     3361 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     3421 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     3481 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     3541 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     3601 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     3661 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     3721 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     3781 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     3841 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     3901 tgataagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3961 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4021 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4081 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4141 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4201 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4261 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4321 gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat tctgcgcggg
     4381 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4441 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4501 ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac gggtggcatc
     4561 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
     4621 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
     4681 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
     4741 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
     4801 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
     4861 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
     4921 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
     4981 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
     5041 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
     5101 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     5161 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
     5221 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
     5281 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5341 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5401 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5461 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
     5521 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5581 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
     5641 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
     5701 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
     5761 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     5821 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     5881 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     5941 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     6001 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6061 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6121 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6181 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6241 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6301 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6361 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6421 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6481 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6541 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6601 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6661 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6721 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6781 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6841 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6901 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6961 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7021 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7081 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7141 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7201 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7261 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7321 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7381 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7441 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7501 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7561 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7621 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7681 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7741 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7801 atgt
//
