LOCUS       sequence-257060-        7702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7702)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 59_pAAV-ProA16-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2146..2155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2152..3078
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3082..3084,3085..3087,3088..3801)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3082..3084,3085..3087,3088..3798)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3127..3148)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3388..3407)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3735..3756
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3829..4417
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3882..3902)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4300..4311)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4449..4925
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4648..4667)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4965..5105
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4965..5094
                     /label=AAV2 ITR
     rep_origin      5180..5635
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5267..5286)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5477..5498
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5652..5671)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5771..5793
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5831..5849)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5917..6021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6022..6090,6091..6882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6240..6259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7053..7641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7542..7561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcttagg ctgcattaac aatgtgtcgt gtccagactg
      181 ggggaaataa taggtctatt gtactggctt ggtcaggcca tatctggaat cttgcgttgc
      241 tacactgaca gactggaatc gggccaggag gggaactagc ccagcctcag ctcctgagtc
      301 cttgtgcgcg aaatgtttcc tgtacaactt cttgtcttat cccccagaca acgctattag
      361 gtaggtccct attttgtaca caaggaggtt aataaagttg ttcaggaaca actagatagc
      421 ccgtccatgg aggtgcctgg ggtttttctg aatgggctgg ctcctctggt gcttcagctc
      481 acactcactg tgatttagtg ggatatggga gtgaaagaga tagagttctt attttcaagg
      541 agggcataat ctggctcggt tattcagatc acttctggaa tgctgccatt caaaggagtg
      601 aagaatttag ggctggaaga gactgtacag gactccatag cttccctcct atagtatgga
      661 agagagagag ggcagtgtga cagtggggac aacaggcctt cttacagtct tcactatttc
      721 ccttggcttg tttctggaca gtgatgtgcc agggcacaca agggtgctgt ggccattgtg
      781 gaggagtcag atctcaatag ctcttccctg caatctgaac taagaggagg ggaaaaaaaa
      841 aaagcttgac actgagaaac acaggtcctg tgtgagcggt ctctgagagt ctaagcagta
      901 acttcactga agagggagag ctggacttga cccaggctcc tggggatctg tgaaggctgg
      961 agtgccatta gcagaggtgg aaggaaaggc agcaaagacc gttctctgct ggcacctatg
     1021 cctggcctct gtttagatga tgacggtcct cccttctttc aaggaccaca ttcctctgca
     1081 tgctctttct caaagctttt cttttctctt ctctcttttc ttttcttttc ttttcttttc
     1141 ttttcttttc ttttcttttc ttttcttttc ttttcttttc gacacagagg gctgctggcg
     1201 tcttgtatct cccagggctt cctgtctggg agcacagaca tagctcagcc ttctgtaatt
     1261 tatgctgagg ggcagagcag gagagttatg agtaaagact ttggaactgg acaaaacctt
     1321 tctggcctgt ctgtgtcgca acagctgttc ttcttgtgtc cccctttcct gggttgtaaa
     1381 atggggataa taatagtacc accctcagat ggcgttcata agcacccagt gaggtcatgt
     1441 taagcctgtt agaaagctac ccataaacgg ctgctgtggc catggctcct tcttagaact
     1501 tgcgagttcc cctgcctgga agcggtagcc actggacttg gtaatttttc tgtcccattg
     1561 aaaaaggccc ctacttttca aagggaggaa gaggaaattg tcgatcttga gacacgaggg
     1621 acattgataa tgaaagttac agcccatggc ctcaggtttg ggggagggga gaagccacct
     1681 gactccggat tttaaaagga gctgatgggg attcagaagt caagggccac aggctctact
     1741 cagggaaaac tcactctggg ggacctcgga caggtcactt tcagcctggg acttcagttt
     1801 cctcgggcga gtcaggtggg tttctcagct ttgcttaggt agttaggagc acgcggtgtg
     1861 ggttgccggc tttgagaacg tggctgagca gcgaggctcc gccagccgag atgggcaggc
     1921 gcgtgcaggg attggctgga ggcgcaggcc cgaaccttta agaggcgcgg aggggacgtg
     1981 ctagggagtt ttcatcgccg gcgttcctgg tttgctaagt gctagcgaac gtagtagtgg
     2041 ctggggctgc gcctaggatc ggagccacag gcgagtgccg gccggcagaa cacaagagca
     2101 tcccggcgtc ccggtcccac gcggcgtggc ccgcgggtcg gatccgccac catggattat
     2161 ggaggcgccc tgagtgccgt tgggcgcgag ctgctatttg taacgaaccc agtagtcgtc
     2221 aatggctctg tacttgtgcc tgaggaccag tgttactgcg cgggctggat tgagtcgcgt
     2281 ggcacaaacg gtgcccaaac ggcgtcgaac gtgctgcaat ggcttgctgc tggcttctcc
     2341 atcctactgc ttatgtttta cgcctaccaa acatggaagt caacctgcgg ctgggaggag
     2401 atctatgtgt gcgctatcga gatggtcaag gtgattctcg agttcttctt cgagtttaag
     2461 aacccgtcca tgctgtatct agccacaggc caccgcgtcc agtggttgcg ttacgccgag
     2521 tggcttctca cctgcccggt catttgcatt cacctgtcaa acctgacggg cttgtccaac
     2581 gactacagca ggcgcaccat gggtctgctt gtgtctgata ttggcacaat tgtgtggggc
     2641 gccacttccg ccatggccac cggatacgtc aaggtcatct tcttctgcct gggtctgtgt
     2701 tatggtgcta acacgttctt tcacgctgcc aaggcctaca tcgagggtta ccacaccgtg
     2761 ccgaagggcc ggtgtcgcca ggtggtgact ggcatggctt ggctcttctt cgtatcatgg
     2821 ggtatgttcc ccatcctgtt catcctcggc cccgagggct tcggcgtcct gagcgtgtac
     2881 ggctccaccg tcggccacac catcattgac ctgatgtcga agaactgctg gggtctgctc
     2941 ggccactacc tgcgcgtgct gatccacgag catatcctca tccacggcga cattcgcaag
     3001 accaccaaat tgaacattgg tggcactgag attgaggtcg agacgctggt ggaggacgag
     3061 gccgaggctg gcgcggtacc catggtgagc aagggcgagg agctgttcac cggggtggtg
     3121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3241 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     3361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     3421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     3481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     3541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     3601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     3661 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     3721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     3781 atggacgagc tgtacaagtg ataagaattc gatatcaagc ttatcgataa tcaacctctg
     3841 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3901 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3961 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4021 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4081 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4141 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4201 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc
     4261 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4321 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4381 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgag
     4441 agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     4501 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     4561 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     4621 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     4681 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     4741 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     4801 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     4861 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     4921 tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat
     4981 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     5041 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
     5101 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5161 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5221 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc
     5281 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5341 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     5401 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5461 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg
     5521 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag
     5581 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg
     5641 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     5701 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     5761 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     5821 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     5881 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5941 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6001 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6061 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6121 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6181 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6241 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6301 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6361 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6421 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6481 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6541 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6601 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6661 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6721 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6781 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6841 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6901 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6961 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7021 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7081 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7141 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     7201 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7261 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7321 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7381 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7441 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7501 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7561 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7621 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7681 tgctggcctt ttgctcacat gt
//
