LOCUS       sequence-257074-       11861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11861)
  AUTHORS   Matthews BJ, Younger MA, Vosshall LB
  TITLE     The ion channel ppk301 controls freshwater egg-laying in the 
            mosquito Aedes aegypti.
  JOURNAL   eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.
  PUBMED    31112133
REFERENCE   2  (bases 1 to 11861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-05-21"; volume: "8. pii: 43963. doi"; pages: " 
            10.7554/eLife.43963"
COMMENT     Sequence Label: Pub-mCD8:GFP-T2A-DsRed:NLS-SV40
FEATURES             Location/Qualifiers
     source          1..11861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(141..146,147..164,165..171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    179..195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     184..206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     203..219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     203..219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        240..258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2828..3493
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
                     "
     CDS             3500..4213
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3530..3558)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4153..4173
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             4217..4270
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4271..4945
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(4451..4471)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4863..4886
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             4955..4975
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5094..5215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5131..5150)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5185..5204
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8984..9003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8985..9003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9010..9027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9010..9026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9019..9041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9167..9622
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9254..9273)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9464..9485
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9689..9708)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9808..9830
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9868..9886)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9954..10058
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10059..10127,10128..10919)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10277..10296)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11090..11678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11579..11598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11832..11849
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
       61 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      121 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      181 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgaa
      241 attaaccctc actaaaggga acaaaagctt tatggtttat gattatcaat ttcgttccca
      301 ttgctggaat atctttcggg ttgtttgtta tgcctcgctt ttcactgcgt gctagcagtt
      361 cccttttcct taggaatgac catgcgcact tccactttcg tgggataaaa atggtaaatc
      421 cggctttttc ttaaagctac caaatgatgt agataggcat cccacagtac gggctccagt
      481 cggtgaatat agaaactatc aggtgtacaa gtatgaaatg tcgttttttt taaatcaaaa
      541 aacacgttaa attttgtgga aaaagaaaaa atcatttatt caaagtattt gccgtcgcta
      601 gctacacatt tttcccatct ctcgggcaat ttgtggattc cacgccagta gaactcatcg
      661 tcttttgcgg cgaaccattc atcgagccat tttttcacac tttcgtaaga atcgaagcgc
      721 tgctcagcga gtgcgtgtcc catcgaagcg aataggtggt aatcggatgg ggccaggtct
      781 ggtgagtaag ccgcatgcgg aagcacttcc caattgagtg tttccaacgt gtcgcgaacc
      841 gctcttgccg tatgtgatgg agcgttgtca tggagaaaaa tgaccctgtg ttgtcttttt
      901 tgatattccg gtcgttttct ctgaagcgca cggttcaaat tgatcaattg ttgttggtag
      961 cgtgccgtat tcaccgtttc gccgggtttc aagagctcat agtaaatgac accgctctga
     1021 tcccaccaaa cacagagcat cgtcttcttg ccaaagcgat tcggtcgagc agtcgatgtg
     1081 gccggttgtc caggatcaac gtatgacttt ttacgtttag gattaacaaa aaagatccat
     1141 ttttcatctc cagtaacgat acgatgcaaa aacgacttcc ttttgtatcg tgaaagcaaa
     1201 atttcgcatg tgtttttgcg cctctccatc tgcctctcgt tcaactcatg tggcacccat
     1261 ctaccgacct tctgaatctt tcccatctct cgcaagcgat tggaaactgc ttgttgactt
     1321 acttccaact gctctgcgag ttgtttttgc gtttgagcat cgtcttcatc caataatgct
     1381 tgcagttcgg cgtcttcgta cctttttggc ggttttccgt gctctttgtc gtcgatatct
     1441 ttacatgtag cttgtgcatt gaatccaatt ataatttgcc ttggcaccag ctgagccaga
     1501 caagaaagaa agcttcccag aagtatatcg atttagaagg gttgacgtca cttgctgact
     1561 gcactaatac agcaaatgat gcaattagaa tgattcaagt gaaattccca aattactgat
     1621 ttttctctgg atttggttat cagattacat tcgaagctaa gattagctac cgaaattgtc
     1681 gatcaaatca ggaaatcctt tctctatcga aaaaggcatt cgcacatctt cctctctatg
     1741 ccatatacac gaagggtagg tacattgacg tctttgccag aagttgaact gcatcgttca
     1801 aggtacagaa tgaacgacta acagacacaa gcacgttttg ctgtccattc agacacaggg
     1861 atggtaccca tattcgatcg atatagagcc atccaaccga acagaggtat atgtatgaat
     1921 gtattgctga aattttctag aagtacaacc accactacga cagtgtctat aaaacgcccc
     1981 tgcaaaggcg aaaccagctc aatcgaatac gtttcctagt ggagtgaaca ttacgcggcc
     2041 caagtaagca gtgccagtgc aagtgaagtg aagtctctag tgaaaaagag tgatccaatt
     2101 agccagagga gaaaatttca gagtgaacaa agctttattc aaaggacaat tactattaaa
     2161 ttggtgaaag tgcatttcgg tgaagggaat cttctagtga aggtaggtaa attaattgat
     2221 gaaattatag ctatgagcga aaactagttt ggtgaatgat tcctttgtct ttgaatgagc
     2281 aaactatttt ccaagatggc gactattgag ctttgagtga ttagtgaaaa tttgcaacgc
     2341 agtttcatca tcattgataa aacccaattg tgattcacag cgataatcat atttcgttga
     2401 atcatcgcta ctaattgaat taaatttcta gaataataag aataacgtat ttgctccgtc
     2461 acatatctaa aataaatatt ttgatggtaa ttacccatta aggtaatatt aacacatatc
     2521 gagaaaaacc ttgaggaaat cgtgaaaact tgaagatacg caatttcaaa actacgtagt
     2581 tcaaagtcga aaacaagtta atttttcact taaaagtagg gcgttgttgt gacgtcatca
     2641 ccttcaagtg tatatttttc acttggcctg cgactgcaaa cgcagacaaa gcaaaacaag
     2701 tttaaaacct gtcgtgtcgt gctcgaagcc aaaggcaatg aatcaatatc aaatgagagt
     2761 ttgcatttca caaccaatta ctcaagcgtt tcctcgtttc tttttctgct caacagagat
     2821 ttcaacaatg gcctcaccgt tgacccgctt tctgtcgctg aacctgctgc tgctgggtga
     2881 gtcgattatc ctggggagtg gagaagctaa gccacaggca cccgaactcc gaatctttcc
     2941 aaagaaaatg gacgccgaac ttggtcagaa ggtggacctg gtatgtgaag tgttggggtc
     3001 cgtttcgcaa ggatgctctt ggctcttcca gaactccagc tccaaactcc cccagcccac
     3061 cttcgttgtc tatatggctt catcccacaa caagataacg tgggacgaga agctgaattc
     3121 gtcgaaactg ttttctgcca tgagggacac gaataataag tacgttctca ccctgaacaa
     3181 gttcagcaag gaaaacgaag gctactattt ctgctcagtc atcagcaact cggtgatgta
     3241 cttcagttct gtcgtgccag tccttcagaa agtgaactct actactacca agccagtgct
     3301 gcgaactccc tcacctgtgc accctaccgg gacatctcag ccccagagac cagaagattg
     3361 tcggccccgt ggctcagtga aggggaccgg attggacttc gcctgtgata tttacatctg
     3421 ggcacccttg gccggaatct gcgtggccct tctgctgtcc ttgatcatca ctctcatctg
     3481 ctaccacagc cgcggatcca tgagtaaagg agaagaactt ttcactggag ttgtcccaat
     3541 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     3601 aggtgacgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     3661 tgttccatgg ccaacacttg tcactacttt aacttatggt gttcaatgct tttcaagata
     3721 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtcca
     3781 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     3841 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     3901 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     3961 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     4021 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     4081 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     4141 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     4201 tgaactatac aaaggagagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     4261 tcccggccct atggcctcct ccgagaacgt catcaccgag ttcatgcgct tcaaggtgcg
     4321 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4381 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     4441 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     4501 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4561 gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg
     4621 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca
     4681 gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     4741 gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     4801 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc
     4861 caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac
     4921 cgagggccgc caccacctgt tcctgagatc tcgacccaag aaaaagcgga aggtggagga
     4981 cccgtaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     5041 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     5101 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5161 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct
     5221 tatcgatacg cgtacggcgc gcctaggccg gccgatctcg tcgacgtcaa aatcaccact
     5281 tttgaagcgt tgaaaccacc gttcacacgt tttcacagtt ggtacttgtt cgccaaaggc
     5341 ttcaacaagc attcggtgcg attccgcagc tgttttcttc aaatgaaaac agaaaattaa
     5401 tactgtccgc gtttgctctt tattcggcac gaaactcgac atgttgactg cactgagagt
     5461 aaacaattat gacgctcaat tcgcgccaaa ctatggtggt tcgacagtca aggttgacac
     5521 ttcacaaggt caaagtttta tgacaatcga taaatattta cgtttgcgag acatctatat
     5581 gttcgaaccg acattcccta cttgtacacc tggtaaaaaa ttaaaactag gtccccgttc
     5641 ttacgcatat atacacaggt acgtgggtgt taattaatta ctttaaaaca ttaaggaact
     5701 tcgattggca tttaacaagg aaactaacat catattgtta tacaaataaa agttgctcat
     5761 ttcttactcg taattcgaaa cctttctcgg ttcttttttt taccatacta tcactttaaa
     5821 gtggttttaa ccaacatgca tacatttcgt ttttttttcg ttttactcgt ctgcgttcgc
     5881 tctttgtttt ttaaccaaag taaatctgac tcagggaagt cactcaaaac atgtttgcct
     5941 gagctctcgt attccaaatg gaactccggg aaaaatatat gctcaaaagt ggctcaaaac
     6001 tgtatctatt cgtattttga tatacacaat aataaaactg ctcgaagaac tccaatctgt
     6061 tacacaatgc ctggtcattg tttacattta gctcacttgt gtacttatgt gcacccatac
     6121 acacccatgt gtagataaag gatggcttat cttatcactg acaaaacagc ggagcatgca
     6181 agaatgctgt ttgtctttgt ccattggctt cgattaagta gtcgaaaagg aaaacggtaa
     6241 atggaatgca taactttgcc ctctcactcc ctctaatcgg acggaagagt cggacgttat
     6301 gacctgtcct gatttggaga aatcttttct atatttcatt tatttcgctt acttccctac
     6361 ttctttcaaa tgcactaatg cgaaaaacat tatttttttc aaattaaatg gtcaagtgtg
     6421 cttctaatga aatccatagt ctcaatacat acatatatgc agtcggcaaa aaaggaggca
     6481 catactcgta gaaatttttc attgctctgt atatggattt gtaaatggta taaaaagcat
     6541 atatcagtat cagttctctt cccattcccg ctctcagtgc gctctctcgg ttgagcagtt
     6601 ttcgagcctc tcgagttcga gttcaccgag ttcgagttgc gacggttcgc ccactcagtt
     6661 tgcccacagc tctgaatgcg agcgtaacga tcgcaagccg cctgtcttgc agtgaagtgc
     6721 attttctcaa gccgcttcct cgatttgtat ttggttggac aacagcccat aacttgaacc
     6781 cgtctttaat gcccgtatct ctgcgagagt gagtttccaa tggcgatttg ccgagtgcgc
     6841 gtgtgcaggg caccctttcc ttcctgttgt cgtccggaga gtattcgcgt tgagtggaac
     6901 agccgtataa gagtggttgg gctagcggat ttaaaggaat gtgggtgctg tgcctttaag
     6961 ttaggactcc ttgtgaaggg atacgcagag cagcggctgg atcgcaagtt atttctaaaa
     7021 atatatggaa agtaatgggg gagatatacg aaatgtatct gcacgaaaac tcatatgctc
     7081 gccagatgtt tagcttgagc aaaaacttca gattccttaa aatttccgtt cttattattt
     7141 attatttctg ctttagcgca ggttttaatc agaacttttt tcccttaata taattggatg
     7201 tgtgtatttg cgcagtgttc ggctctccca gattttctgt ttttaatttt ttcccttttc
     7261 ttttttaata agaaagtttt gaagtttgat tacattcttt cctatatctt tcgattctat
     7321 aaactttaca tattggtcta gacgcgtggt tccatccaat tgagagtgaa ctattcgatt
     7381 tcttatatta agttgctctt ccggtggctc agacacagaa gttgagaaac gagaagtttt
     7441 aaaacacaca ctatccggac aactagacat actttcgaaa tctgctgttt gccgtttttt
     7501 gcataggaag gctgtttgtt ttttggtcaa attgtaaaaa tatttgcttt tctttaggtg
     7561 ggctttggac ttcgcttttt cgttggcaat aactattttt gtaagacttc tggacatctt
     7621 tgtcctgtag ttatcgaaat taatcggggt gcgcaacaag atgtgataac ctgcgttcgt
     7681 ctgaaaaatc tcagatagtt cgccatcatt cagcaacaaa atgtttctct caaagacgaa
     7741 agacgtttgt gtaaggctca aggggcccaa gtcaccgcca tgccgggcgg agcagcagtc
     7801 ggagatcatt tttgccaatt cagcaaactc acatttgcct gattgaatta gatccctcgc
     7861 atgcattatc ttatttagcg cttcctgcct cgttctcctt accacccgtt cacgatatga
     7921 actacacctg tccgattcgt tgtgcttcac caggatgtgt ctgcagcgga gcgtgcattt
     7981 gtcattgcac tcagggtagt ccccaagaac ggcgccccag gcattcctat tcttttctcg
     8041 gtaggggctt ggaggcagtg tgaaatatac cttcctggta attgtgtcgt agaagtagca
     8101 ctccttggta ctgtgaacaa ttcgctcttc ccatccaagg ggcagcttgt taggcctctc
     8161 agaattatga cgttcgccag ttaggatcga gagcccactg tccgcatcac cataggagtc
     8221 gcttttaacg ttatcactcg aatatttact tttagagctc gcgtctaacc aaccccaaga
     8281 gtggtcacaa gtttgctgat cttcattttg gccatcttct attttaaata tatcaaactt
     8341 gcatagcgcc ggcttcatgg tgttcatgtt tgcacaaacc agacggtata ggtatttatg
     8401 tgttaagtat agatactgaa taggttactg ctttgagcca aactcttaaa gaaaaataaa
     8461 gcaatgcaat cttgtttgtt ttacagctcg gctttaaggc atatattttt aattatgttt
     8521 tattttcatg cttttatttt tttttaggtt tccgaaatag ttatgaatct tatactgact
     8581 gtaaccacgg ctctgcacct agttttggtg gtattggcgc acaatccgcc gtcgattcgt
     8641 ggcgaaagcc attctgatgg cattcgtctg gtcatggaaa agaggtcggg cgatgtagta
     8701 cacattcgtc cagctcgggg caccctggag gacgaaacag tcgcatccac atctacaaca
     8761 aaaagtgaca cccaccgcac caggaataat cgtaagccga acaagccgca agagcactct
     8821 cagcaggttg gggcccacaa gtcgggatct agaaaactag tggttgcgga aaatggcggg
     8881 gcactgtcat cacagctacc acaaccaaat cacaaacaag gccacaagaa cactgataag
     8941 caacaacgag gaggaggatc cccgggtacc gagctcgaat tcgccctata gtgagtcgta
     9001 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     9061 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     9121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggacgc gccctgtagc
     9181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     9241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9361 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     9421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9481 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     9541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     9601 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     9661 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     9721 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     9781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     9841 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     9901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     9961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    10081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    10141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    10201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    10261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
    10321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    10381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    10441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    10501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    10561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    10621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    10681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    10741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    10801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    10861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    10921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    10981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    11041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    11101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    11161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    11221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    11281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    11341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    11401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    11461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    11521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    11581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    11641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    11701 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    11761 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    11821 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga g
//
