LOCUS       sequence-257078-        8747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s 
            calcium indicator, separated by T2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Matthews BJ, Younger MA, Vosshall LB
  TITLE     The ion channel ppk301 controls freshwater egg-laying in the 
            mosquito Aedes aegypti.
  JOURNAL   eLife. 2019 May 21;8. pii: 43963. doi: 10.7554/eLife.43963.
  PUBMED    31112133
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-05-21"; volume: "8. pii: 43963. doi"; pages: " 
            10.7554/eLife.43963"
COMMENT     Sequence Label: 15x-QUAS-dTomato-T2A-GCaMP6s
FEATURES             Location/Qualifiers
     source          1..8747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   104..166
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      652..661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(658..660,661..663,664..1377)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1500..1621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2033..2271
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     2218..2239
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             2347..3048
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2480..2498)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2748..2767
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2942..2965
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             3052..3105
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3106..3114,3115..3132,3133..3135,3136..3168,3169..3171,
                     3172..3195,3196..3222,3223..3279,3280..3285,3286..3555,
                     3556..3573,3574..4008,4009..4014,4015..4458)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      3202..3211
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          4535..4600
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4730..4750
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5022..5156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5431..5465
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5956..5972)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5956..5972)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5969..5991)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5980..5996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6004..6010,6011..6028,6029..6034))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6049..6070
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6187..6204)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6358..6946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6438..6457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7117..7908,7909..7977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7740..7759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7978..8082)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8108..8563)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8245..8266)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8457..8476
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8690..8712
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8704..8721
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8705..8721
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca
       61 gatgaagatg ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca
      121 tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct
      181 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
      241 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac
      301 aaaaactcaa aatttcttct ataaagtaac aaaactttta tcgaattcct gcagcccggg
      361 ggatccacta gttctagtgt tcccacaatg gttaattcga gctcgcccgg ggatctaatt
      421 caattagaga ctaattcaat tagagctaat tcaattagga tccaagctta tcgatttcga
      481 accctcgacc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
      541 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      601 caagctaaac aatcggggta ccgctagagt cgacggtacg atccaccggt cgccaccatg
      661 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      721 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      781 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      841 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      901 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      961 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1021 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1081 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1141 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1201 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1261 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1321 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     1381 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     1441 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     1501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     1561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     1621 aaagcttatc gatacgcgta cggcactaga gcggccgcca ccgcggtcac gtgtcactgg
     1681 gtcagtgggt aatcgcttat cctcggataa acaattatcc tcacgggtaa tcgcttatcc
     1741 gctcgggtaa tcgcttatcc tcgggtaatc gcttatcctt gggtaatcgc ttatcctcgg
     1801 ataaacaatt atcctcacgg gtaatcgctt atccgctcgg gtaatcgctt atcctcgggt
     1861 aatcgcttat ccttgggtaa tcgcttatcc tcggataaac aattatcctc acgggtaatc
     1921 gcttatccgc tcgggtaatc gcttatcctc gggtaatcgc ttatcctttc acgttgggac
     1981 tcagtgagga ggacctgaat tcctgcagcc cgagcggaga ctctagcgag cgccggagta
     2041 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     2101 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     2161 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     2221 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     2281 ttgggaattc gttaacagat cgggggatca attcgttaac agatctgcgg ccgcggctcg
     2341 agcaaaatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
     2401 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     2461 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     2521 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
     2581 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     2641 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     2701 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     2761 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     2821 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccgctacct ggtggagttc
     2881 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     2941 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     3001 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaaggg agagggcaga
     3061 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatggg ttctcatcat
     3121 catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg ggatctgtac
     3181 gacgatgacg ataaggatct cgccaccatg gtcgactcat cacgtcgtaa gtggaataag
     3241 acaggtcacg cagtcagagc tataggtcgg ctgagctcac tcgagaacgt ctatatcaag
     3301 gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa catcgaggac
     3361 ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3421 ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag
     3481 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3541 gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga ggagctgttc
     3601 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     3661 gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     3721 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     3781 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     3841 cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     3901 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     3961 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct gccggaccaa
     4021 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg
     4081 gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc
     4141 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc
     4201 gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga cacggaagaa
     4261 gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat cagtgcagca
     4321 gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa
     4381 atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa
     4441 atgatgacag cgaagtgaga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     4501 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     4561 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     4621 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     4681 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     4741 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     4801 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     4861 actgctatac aagaaaatta tggaaaaata tttgatgtat agtgccttga ctagagatca
     4921 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     4981 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5041 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5101 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcgata
     5161 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     5221 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
     5281 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
     5341 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
     5401 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
     5461 tagggttaat ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc
     5521 gcccaagctg gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg
     5581 ttgaagcggc atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa
     5641 aacgcacgtt taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac
     5701 tgttcgttca ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga
     5761 tggtcagccc gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg
     5821 ccggtgtgca gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt
     5881 atcctggctg gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg
     5941 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6001 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6061 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6121 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6181 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6241 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6301 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6361 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6421 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6481 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6541 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6601 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6661 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6721 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6781 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6841 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6901 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6961 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7021 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7081 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7141 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7201 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7261 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7321 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7381 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7441 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7501 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7561 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7621 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7681 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7741 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7801 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7861 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7921 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8101 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8161 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8221 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8281 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8341 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8401 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8461 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8521 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     8581 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8641 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8701 cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttt
//
