LOCUS       sequence-257094-        9394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lck FRET Biosensor. To visualize Lck kinase activity in mammalian 
            cells by FRET.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Wan R, Wu J, Ouyang M, Lei L, Wei J, Peng Q, Harrison R, Wu Y, Cheng
            B, Li K, Zhu C, Tang L, Wang Y, Lu S
  TITLE     Biophysical basis underlying dynamic Lck activation visualized by 
            ZapLck FRET biosensor.
  JOURNAL   Sci Adv. 2019 Jun 19;5(6):eaau2001. doi: 10.1126/sciadv.aau2001. 
            eCollection 2019 Jun.
  PUBMED    31223643
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-06-19"; volume: "5(6):eaau2001. doi"; pages: " 
            10.1126/sciadv.aau2001. eCollection 2019 Jun"
COMMENT     Sequence Label: ZapLck biosensor
FEATURES             Location/Qualifiers
     source          1..9394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    204..321
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        450..1628
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          681..1619
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1576..1596
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1651..1660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1657..1659,1660..1662,1663..2175)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(1702..1723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1963..1982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2310..2331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2740..3450
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
     misc_feature    join(3550..4124,4125..4127,4128..4136)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3717..3734)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3944..3963
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4156..4755
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4156..4175)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4652..4672
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4958..5191
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5342..5359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5348..5572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      5615..5624
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5668..5772
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5773..5841,5842..6633)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5991..6010)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6804..7392
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7293..7312
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             7551..8184
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8231..8356
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8849..9082
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9267..9311
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
       61 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
      121 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca
      181 caaatggcag tattcatcca caattttaaa agaaaggggg ggattggggg gtacagtgca
      241 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      301 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggatc
      361 gataagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
      421 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
      481 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
      541 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      601 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      661 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
      721 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
      781 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
      841 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
      901 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
      961 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     1021 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     1081 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     1141 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     1201 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     1261 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     1321 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     1381 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     1441 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     1501 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     1561 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     1621 tgtcgtgagg aattgatcct tcgaactagt gccaccatgg tgagcaaggg cgaggagctg
     1681 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaggttc
     1741 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1801 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     1861 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1921 atgcccgaag gctacgtcca ggagcgtacc atcttcttca aggacgacgg caactacaag
     1981 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2041 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacatcagc
     2101 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccca cttcaagatc
     2161 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2221 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2281 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2341 cgcatgcatt ggtattttgg gaagatcact cgtcgggagt ccgagcggct gctgctcaac
     2401 cccgaaaacc cccggggaac cttcttggtc cgggagagcg agacgacaaa aggtgcctat
     2461 gccctctccg tttctgactt tgacaacgcc aaggggctca atgtgaagca ctacaagatc
     2521 cgcaagctgg acagcggcgg cttctacatc acctcacgca cacagttcag cagcctgcag
     2581 cagctggtgg cctactactc caaacatgct gatggcttgt gccaccgcct gactaacgtc
     2641 tgtgggtcta catctggatc tgggaagccg ggttctggtg agggttctat ggacacgagc
     2701 gtgttcgaga gcccctacag cgacccagag gaggagctct ctaaaggtga agaattattc
     2761 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     2821 gtctccggtg aaggtgaagg tgatgctacg tacggtaaat tgaccttaaa attactctgt
     2881 actactggta aattgccagt tccatggcca accttagtca ctactttagg ttatggtgtt
     2941 caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     3001 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     3061 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     3121 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     3181 aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt caaaattaga
     3241 cacaacattg aagatggtgg tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     3301 ggtgatggtc cagtcttgtt accagacaac cattacttat cctatcaatc tgccttattc
     3361 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttttgac tgctgctggt
     3421 attaccgagg gtatgaatga attgtacaaa taagaattcg aagcttgagc tcgagatctg
     3481 agtccggtag cgctagttcg aaggatccgc atgcatctag ggcggccaat tccgcccctc
     3541 tccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     3601 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     3661 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     3721 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     3781 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     3841 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3901 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3961 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     4021 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     4081 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca
     4141 aggagacgac cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4201 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4261 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     4321 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     4381 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     4441 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     4501 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     4561 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     4621 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     4681 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     4741 aagcccggtg cctgataata ggcggccgct cgagacctag aaaaacatgg agcaatcaca
     4801 agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag
     4861 gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct
     4921 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4981 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5041 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5101 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5161 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     5221 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttataataga
     5281 tttagcagga attgaactag gagtggagca cacaggcaaa gttctagagc tcgctgatca
     5341 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5401 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5461 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5521 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     5581 gcggaaagaa ccagctgggg gcgcgcccct cgaggccgcc atggtcatag ctgtttgacg
     5641 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     5701 cattcaaata tgtatccgct cacgagacaa taaccctgat aaatgcttca ataatattga
     5761 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5821 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5881 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5941 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     6001 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     6061 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     6121 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     6181 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     6241 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     6301 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     6361 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     6421 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     6481 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     6541 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     6601 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     6661 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     6721 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6781 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6841 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6901 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6961 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7021 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7081 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7141 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7201 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7261 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7321 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7381 agcctatgga aaaacgccag caacgcggcc tttttacggt tctggccttt tgcacggacc
     7441 gccactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     7501 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     7561 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7621 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     7681 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     7741 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7801 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     7861 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     7921 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     7981 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8041 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8101 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8161 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     8221 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     8281 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     8341 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8401 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8461 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8521 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8581 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8641 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     8701 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8761 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     8821 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     8881 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     8941 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     9001 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     9061 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     9121 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     9181 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     9241 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     9301 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     9361 taatgatagt aggaggcttg gtaggtttaa gaat
//
