LOCUS       sequence-257098-        7708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 76_pAAV-ProA25-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     RBS             1610..1618
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      2152..2161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2158..3084
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3088..3090,3091..3093,3094..3807)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3088..3090,3091..3093,3094..3804)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3133..3154)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3394..3413)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3741..3762
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3835..4423
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3888..3908)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4306..4317)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4455..4931
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4654..4673)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4971..5111
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4971..5100
                     /label=AAV2 ITR
     rep_origin      5186..5641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5273..5292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5483..5504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5658..5677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5777..5799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5837..5855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5923..6027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6028..6096,6097..6888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6246..6265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7059..7647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7548..7567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgccgtagg gatctaacag ccctccttgg cctttgtgag
      181 tgcttcgagc atatggcaca cagacataca tgcaggcaaa acactagtct gtatagactg
      241 atatagaata ctagatagaa taatcttttt ataaaaacta gaaccagctg catctaattc
      301 cagctctcga gaggcagagg caggcagatc tatctctgtg agtctgaggc cagcctggtc
      361 tacatagtga gttctaagac atattagggc tagagagaga gagagagacc ctgtctcaaa
      421 caaacaaaca aacaacaatg ctagaaccat tgagccattt ccacctctgt attataaaga
      481 cccttggaag aaatgtgcat ttgtatgcta tgtactgtgc tccaagcttg atactaagga
      541 aaatgtaagg gagggtcctg acccttcaag tgttgattct cttgaaggaa aaaaaaacaa
      601 aaaacaaaaa accagctcat aaaaaagtaa tcaactttaa aaatcatata aaaccatata
      661 aaaagtattc aaagggaaac atcagattcc ttgaaagtca aggccaataa tgctcaggta
      721 tgccggaggc cagctggact aggaaagagg cgggcaggct tggctttggt tacccacgtt
      781 cagaactagt agtattggct caactcaccg agctcctctg ttgtgcaagc tttgctgcag
      841 aagcacatga gaaagaatca attatatctc caccatgact gcctggggag aaatattttg
      901 gtgtctgtag tttactcgcg gggaaattaa ggcacagaga cataggattc tagaacaggg
      961 ggtctggctc tggagtctct ttttttttcg gggggggggg ggggggrggg gaggttcgag
     1021 acagggtttt tctgtgtagt cctggctgtc ctggaactca ctcggtagac taggttgacc
     1081 ttgaactcag aaatccgcct gcctctgcct ctcaagtgct gggattaaag gagtgcgcca
     1141 ccaccgccca gcccggagtc ttttacaccg taggatcttc ggaatctagc acaattatcc
     1201 ggtgctgagc gttaaaagca aaacaacaac caacctagct ttccttgaaa attgaatttt
     1261 taggataatc caattcaagg ctgaaatttg taaataaata aacaagtaaa taaagaaagc
     1321 attttagtat tatagacctc ccaccgatcc tctccccacc tctaatcaaa ggccagcaag
     1381 aaaaaaaaat tttatttata tgtttttttt cctccccttt tgtgatgctt tggagtttct
     1441 gaacgctagg ggctccctga ttctctggtt tgagaacaac tttttctttt acgatttttt
     1501 tttttttttt ttttttggaa aaggggtggg ggaaatgtgg ccttaattat cctacgtttt
     1561 aggcagctta aggaaggggc tgtgctttct gaatcttgtt ggagccaagt aaggaggtcc
     1621 ttctcctccc ccctcgcttt ttaaaggcgc tcgtgaaata taagctcaga aaacaaaccg
     1681 agcgatcaca gcggcagccc gggcggcagc agcaggagga ggaggagcag gaggaggagg
     1741 cagagttgga gttgggcaga cgctgaggag agctagctgc aggacgctgc ggagttgcag
     1801 ggctccgcgc tgctccattc attaagtagc tgcggaggat cgtggcggga cgccgacgct
     1861 gcccactcgc aaactccagc acgcagacgc acgccagccg gacgcgtccg gggcagcaca
     1921 gttctgcaaa agttcctgct cgggatttgg ctctcttccc cttggactag cgaacagttt
     1981 ggggttgaga ggaaagcaga ggcgcccaga ggggaaacct acccgcgtct gcagttggaa
     2041 ctactccgcc gggctgcact ctcgccgccg cgtcccgagc ccgcataccc gctcctgagc
     2101 agcggcggcc cgcatcgccc cgggaggctc cccgcagcca gcgccggatc cgccaccatg
     2161 gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac gaacccagta
     2221 gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg ctggattgag
     2281 tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct tgctgctggc
     2341 ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac ctgcggctgg
     2401 gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt cttcttcgag
     2461 tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg gttgcgttac
     2521 gccgagtggc ttctcacctg cccggtcatt tgcattcacc tgtcaaacct gacgggcttg
     2581 tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctgatattgg cacaattgtg
     2641 tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt ctgcctgggt
     2701 ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga gggttaccac
     2761 accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta
     2821 tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc
     2881 gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt
     2941 ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt
     3001 cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag
     3061 gacgaggccg aggctggcgc ggtacccatg gtgagcaagg gcgaggagct gttcaccggg
     3121 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3181 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3241 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3301 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3361 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3481 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3541 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3601 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3661 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3721 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3781 ctcggcatgg acgagctgta caagtgataa gaattcgata tcaagcttat cgataatcaa
     3841 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3901 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3961 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4021 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4081 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4141 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4201 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat
     4261 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4321 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4381 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg
     4441 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     4501 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     4561 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     4621 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     4681 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     4741 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     4801 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     4861 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     4921 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     4981 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     5041 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     5101 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5161 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     5281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5401 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     5521 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     5581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5641 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5701 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5761 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5821 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5881 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6241 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6541 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7681 gccttttgct ggccttttgc tcacatgt
//
