LOCUS       sequence-257116-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 Part. Insertion site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Vasudevan R, Gale GAR, Schiavon AA, Puzorjov A, Malin J, Gillespie 
            MD, Vavitsas K, Zulkower V, Wang B, Howe CJ, Lea-Smith DJ, McCormick
            AJ
  TITLE     CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria 
            Based on the Plant MoClo Syntax.
  JOURNAL   Plant Physiol. 2019 May;180(1):39-55. doi: 10.1104/pp.18.01401. Epub
            2019 Feb 28.
  PUBMED    30819783
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.18.01401"; journalName: "Plant Physiol"; date: 
            "2019-05"; volume: "180"; issue: "1"; pages: "39-55"
COMMENT     Sequence Label: pC0.167
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggagttt gctgaagcgt taaagttaga agtcccgatt atttccctca
     2281 ccagggatgg ttttgcggaa gcgcaagttt tactggcagg tttgcagaac tacggagagc
     2341 accaaattgt gccccaccag caatttttcg ccggcaactg ggattttttg ttggaaagcc
     2401 cccaaaaacc taggttggat cagaagttag acaaacatgg cgatcgccag atagcagaaa
     2461 aaatcgtcgc ccaaattagt tgattttgtc ccccaactcg gaaaaagtca ggggattttg
     2521 gtaaaccata aacctggggt taacctctgg gaagccgttc atggttgttc atgccgtatg
     2581 cttttacgat ctaaatgcta tctgacacct ccgttgactt aataaaactt aatccaaata
     2641 atttgactta gtggttcttt tcaggcattc tctcagcatt agaaggctga gtactgagag
     2701 atcccctcat aatttcccca aagcgtaacc atgtgtgaat aaattttgag ctagtagggt
     2761 tgcagccacg agtaagtctt cccttgttat tgtgtagcca gaatgccgca aaacttccat
     2821 gcctaagcga actgttgaga gtacgtttcg atttctgact gtgttagcct ggaagtgctt
     2881 gtcccaacct tgtttctgag catgaacgcc cgcaagccaa catgttagtt gaagcatcag
     2941 ggcgattagc agcatgatat caaaacgctc tgagctgctc gttcggctat ggcgtaggcc
     3001 tagtccgtag gcaggacttt tcaagtctcg gaaggtttct tcaatctgca ttcgcttcga
     3061 atagatatta acaagttgtt tgggtgttcg aatttcaaca ggtaagttag ttgctagaac
     3121 ccatggctcc tttgccgacg ctgagtagat tttaggtgac gggtggtgac aatgagtccg
     3181 tgtcgagcgc tgattttttc ggcctttaga gcgagattta tacaatagaa tttggcatga
     3241 gattggattg cttttagtca gcctcttata gcctaaagtc tttgagtgac tagatgacat
     3301 atcatgtaag ttgctgatag gtttccagtt ttccgctcct aggtctgcat attgtacttt
     3361 tcctcttact cgacttaacc agtaccaacc cagcttctca acggatttat accatggcac
     3421 tttaaagcca gcatcactga caatgagcgg tgtggtgtta ctcggtagaa tgctcgcaag
     3481 gtcggctaga aattggtcat gagctttctt tgaacattgc tctgaaagcg ggaacgcttt
     3541 ctcataaaga gtaacagaac gaccgtgtag tgcgactgaa gctcgcaata ccataagtcg
     3601 tttttgctca cgaatatcag accagtcaac aagtacaatg ggcatcgtat tgcccgaaca
     3661 gataaagcta gcatgccaac ggtatacagc gagtcgctct ttgtggaggt gacgattacc
     3721 taacaatcgg tcgattcgtt tgatgttatg ttttgttctc gctttggttg gcaggttacg
     3781 gccaagttcg gtaagagtga gagttttaca gtcaagtaat gcgtggcaag ccaacgttaa
     3841 gctgttgagt cgttttaagt gtaattcggg gcagaattgg taaagagagt cgtgtaaaat
     3901 atcgagttcg cacatcttgt tgtctgatta ttgatttttc gcgaaaccat ttgatcatat
     3961 gacaagatgt gtatccacct taacttaatg atttttacca aaatcattag gggattcatc
     4021 agggctgagt atgggtttta atgctgttta tccttacaag ttaagtgata aatgtgcatt
     4081 aaggattccc attgcagtct aattactggt tagctgcaaa taaaaaatga aaaaaccaga
     4141 tattgatgtg tattgcaatg taatggaaga aatcaagcga cgaacaggcg ttatcgattt
     4201 ttttctatct ggtaatggtc acgctttatg tgaacctaca acactggaat caattgcgct
     4261 gcaaatcagg aaaatcctag agctaatagc tatggcgtca cttgtagcaa acgaaaaaga
     4321 atacaaaaag gtatattcca attttgccag cgcatggaat gcagaatatt tactaaatgt
     4381 gagaccacga a
//
