LOCUS       sequence-257130-       11121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAL4-induced expression of codon-optimised FLP recombinase with 
            unfused mNeongreen (mNG) as a marker in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ayuso C, Askjaer P
  TITLE     Spatiotemporal control of genome recombination through combined 
            FLP-Frt and GAL4-UAS technologies.
  JOURNAL   MicroPubl Biol. 2019 Jan 29;2019:10.17912/micropub.biology.000089. 
            doi: 10.17912/micropub.biology.000089.
  PUBMED    32550473
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2019-01-29"; volume: 
            "2019:10.17912/micropub.biology.000089. doi"; pages: " 
            10.17912/micropub.biology.000089"
COMMENT     Sequence Label: pBN449
FEATURES             Location/Qualifiers
     source          1..11121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2265..2285
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3332..3356
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3470..3564
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3603..3697
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3736..3830
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             9317..9328
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9477..9501)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10962..10982)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(11002..11018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11002..11018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11015..11037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcaaaagacc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctgacc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg cactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttatcaacta tgtataataa
     2281 agttgggccc attaggtcag acaaaaattt ctgatcttta aaccttattg attaaaacta
     2341 atttttgccc gacttttttg acacatgcac tagacgcttg tactcaaaat ttggaaacct
     2401 tgctcaaatc cactactcag atatgacagt agcatcgata attgaaccat tatcctgaaa
     2461 tcccctttcc tgtcccggtt cattttccaa ttttttcaac ggtggaagaa ggggcaaatt
     2521 ttataccaaa acgtctccgt ttatgaccca ccccctatgc aaaataaacg gctctgggag
     2581 aaatagtaca gaaaacgcgg agcctgcttg attccttctc cgaggcgaca aaaaacgcat
     2641 cattcgtatt gtaagatgca tgtgtggctg gcctcttttg aattttactg gttttttata
     2701 tttctactaa tatttcgaaa aattttgtaa gtaaatcatt gattacgttt acaaaaattt
     2761 agaaacattt caaaatgatt acttctaaat taattatatt tacaggttag ttcgagttac
     2821 ttatgacctt ccaatccgcc atatccgcca aggacgctcc aattaatttt ttagaattat
     2881 tttttaacac atgtattatt ttttaacaat aaattattca tgaaatcatc aaatgtatat
     2941 gttctactat aatatttagg tcaaactata gaaaagaaat ccttgttttt ggaacaaagg
     3001 agttcagatc ctgtgcattt gatcttggat ccacatcttt ggtccatggg aaagcttggc
     3061 actgatcaag aaggattctt caaatatcta aaattgccgg ctgcccgtag aattccatca
     3121 gttttccaga gttttagtga aaatttgcat cccgttagaa acaatctaca caacggtaaa
     3181 aaggacccat tatttttacc gtaccgtttt cctttatggc cctctatgca aaaaattata
     3241 gcgtggaggg gcagtacaga ataataaagt gtgagaaaaa aaactgctct gcgagaaata
     3301 gtacagcaaa cgcggagcat gcttgattct atccactttg tacaagaaag ctgggttaat
     3361 acgactcact agtgggcaga tcttcgaatg catcgcgcgc accgtacgtc tcgaggagcc
     3421 ggctgtagca tgcggccctt gctagcccat gattacgcca agctacgggc ggagtactgt
     3481 cctccgagcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct
     3541 ccgagcggag tactgtcctc cgagcggaga ctccctagcc catgattacg ccaagctacg
     3601 ggcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     3661 ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactcccta gcccatgatt
     3721 acgccaagct acgggcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3781 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagactcc
     3841 ctaggggcgg ccgcactgac tgggccggcc aaaaatgcat aaggttttgc tggcactgtt
     3901 ctttatcttt ctggcaccag catccgcact ggcagtctcc gaaccggcct gcaggtcgat
     3961 tttttgcaaa ttacgagcgt tgtagggggc ggagcgatag gtcctatagg ttttggtata
     4021 tcatcattca ttcattcatt ggtacattca tttacccacc ttcctctttc tgagcttctc
     4081 tggagttctg tgcttccttt ttcccttatc tttatactgt aatttttaac tttcaggcat
     4141 tgattggcgc gcctctagag gatccccggg attggccaaa ggacccaaag gtatgtttcg
     4201 aatgatacta acataacata gaacattttc aggaggaccc ttggctagcg tcgacggtac
     4261 caaaaatgcc acaattcgac attctctgta agacaccacc aaaagtcctc gtccgtcaat
     4321 tcgtcgagcg tttcgagcgt ccatccggag agaagatcgc cctctgcgcc gccgagctca
     4381 cctacctctg ctggatgatc acccacaacg gaaccgccat caagcgtgcc accttcatgt
     4441 cctacaacac catcatctcc aactccctct ccttcgacat cgtcaacaag tccctccaat
     4501 tcaagtacaa gacccaaaag gccaccatcc tcgaggcctc cctcaaggta agtttaaaca
     4561 tatatatact aactaaccct gattatttaa attttcagaa gctcatccca gcctgggagt
     4621 tcaccatcat cccatactac ggacaaaagc accaatccga catcaccgac atcgtctcct
     4681 ccctccaact ccaattcgag tcctccgagg aggccgacaa gggaaactcc cactccaaga
     4741 agatgctcaa ggccctcctc tccgagggag agtccatctg ggagatcacc gagaagatcc
     4801 tcaactcctt cgagtacacc tcccgtttca ccaagaccaa gaccctctac caattcctct
     4861 tcctcgccac cttcatcaac tgcggacgtt tctccgacat caagaacgtc gacccaaagt
     4921 ccttcaagct cgtccaaaac aagtacctcg gagtcatcat ccaatgcctc gtcaccgaga
     4981 ccaagacctc cgtctcccgt cacatctact tcttctccgc ccgtggacgt atcgacccac
     5041 tcgtctacct cgacgagttc ctccgtaact ccgagccagt cctcaagcgt gtcaaccgta
     5101 ccggaaactc ctcctccaac aagcaagagt accaactcct caaggacaac ctcgtccgtt
     5161 cctacaacaa ggccctcaag aagaacgccc catactccat cttcgccatc aagaacggac
     5221 caaagtccca catcggacgt cacctcatga cctccttcct ctccatgaag gtaagtttaa
     5281 acatgatttt actaactaac taatctgatt taaattttca gggactcacc gagctcacca
     5341 acgtcgtcgg aaactggtcc gacaagcgtg cctccgccgt cgcccgtacc acctacaccc
     5401 accaaatcac cgccatccca gaccactact tcgccctcgt ctcccgttac tacgcctacg
     5461 acccaatctc caaggagatg atcgccctca aggacgagac caacccaatc gaggagtggc
     5521 aacacatcga gcaactcaag ggatccgccg agggatccat ccgttaccca gcctggaacg
     5581 gaatcatctc ccaagaggtc ctcgactacc tctcctccta catcaaccgt cgtatctaac
     5641 gcgtgctgtc tcatcctact ttcacctagt taactgcttg tcttaaaatc tatgcttctc
     5701 tttagtatct aaaattttcc tagaagctta caagtatata aatggtctct tctcaataaa
     5761 ggttgtatat ttattcatct tattgaatct gccatttcct cgtttttgcg agtttatata
     5821 ccttccaatt ttctttctat tgtattttca acttctaatt ttaattcagg gaaactgctt
     5881 caacgcatca tggtgtcgaa gggagaagag gataacatgg cttcactccc agctacacac
     5941 gaactccaca tcttcggatc gatcaacgga gtggatttcg atatggtcgg acaaggtaag
     6001 tttaaacata tatatactaa ctaaccctga ttatttaaat tttcaggaac tggaaaccca
     6061 aacgatggat acgaggaact caacctcaag tcgacaaagg gagatctgca attctcgcca
     6121 tggattctcg tgccacacat cggatacgga ttccaccaat acctcccata cccaggtaag
     6181 tttaaactga gttctactaa ctaacgagta atatttaaat tttcagatgg aatgtcacca
     6241 ttccaagctg ccatggtgga tggatcggga taccaagttc accgaacaat gcaattcgag
     6301 gatggagcct cgctcacagt gaactaccga tacacatacg agggatcgca catcaaggta
     6361 agtttaaaca gttcggtact aactaaccat acatatttaa attttcaggg agaggctcaa
     6421 gttaagggaa caggattccc agctgatgga ccagtgatga caaactcact cacagctgct
     6481 gattggtgcc gatcgaaaaa gacataccca aatgataaga caatcatctc gacattcaag
     6541 tggtcgtaca ctactggaaa cggaaagcga taccgatcga cagcccgaac aacatacaca
     6601 ttcgctaagc caatggccgc caactacctc aaggtaagtt taaacatgat tttactaact
     6661 aactaatctg atttaaattt tcagaatcaa ccaatgtacg tgttccgaaa gacagaactc
     6721 aagcactcaa agacagagct gaacttcaaa gagtggcaaa aggccttcac agatgtgatg
     6781 ggaatggatg aactctacaa gtaacgcgta tgtatctgca tatccttcaa atgttgtcca
     6841 tattgtatca gtaaattata atgccccctt tttatatatt ctccttatca tgtaaatgtt
     6901 ctattgattt tttgctggtg taaaacgttt ttatagtaaa tcctctccac agtgaatttg
     6961 ttaaattgta tcaaaacaag acattgatta aatttgttcg agacttgtgg attgtaaaat
     7021 attttgcaga aaatggcaat attcatttag aaacattact tccataatag gaaaaactat
     7081 ttatgagttt tcatgtgcct atgcaagttc ccactctgtg tgaatttcat catacaaatt
     7141 gagcaaacat aaggtttatg tccagtatgg gttctcagat gttttgtaag agttgagctg
     7201 tcggtgaatg ccttctcaca ttgagcacat ggatacggtc tctctccagt gtgtgttctg
     7261 aaataatgaa aaatagtctt gcaatatgtt tctactattc gagaaaaaat gtaatttttt
     7321 ttcttatggt aacccggctt tccccgggcc tagttctaga cattctctaa tgaaaaaatc
     7381 tttcagttga aattgaaaat gagttaaagt tggagttttt attgaaaaca gatttccgtg
     7441 tgattagtgt ttttagcgag tgtgacagga cagcgaaaaa atatagaaac aaggggggaa
     7501 ctgaaaagct taggaatgca ttgaacatga gaaggggaag gggaaggaac aaactagaca
     7561 ggaattattg gaatttaatc acatttggag ttttttttct attcgacaga ataattatcc
     7621 agaacatttt tgtattaaat atttatgcat catatgagta gtcggctttg ttgtgcatga
     7681 cgagtttgtt atcgacgaaa tagaagctgt cagaacgagt ctcgtttgga ttgttgatca
     7741 tgtcgtccac tgaaaaagag attagtcttt tgaattgtac tttttagaat aatgactcac
     7801 tgagttgttg agagagttga gggaactcat agatatgttc acagttgttt cgtgaattcg
     7861 gaatacagaa tccgaattca aagtcaaaac acttcagaag gcgatctttg aagaagtgac
     7921 gttcgatcat tcggaaatga tggattggga tgtctcctac tttgaattcc acagttgctc
     7981 cgaccgtttt gagtttcaaa aagtttggag caaatctata acgcacgtat cttgccgact
     8041 cctgtggcga ctcatcattc tcctgatcgt tctccggttt ggcgatctcg aaaagcactt
     8101 gctcagtgtc cagatcacgg atttggaact tggtgaactc gatgttatag atgttcgcag
     8161 atggggagca taagaatcct aaatttatgt tttaaactga aatccaaagg gagcaagata
     8221 ccttgagtga ttcccggaag tgctaaaacg tcgttcggag tgatttgagc tttcttcgca
     8281 agctccgatt ccgttgtgat tccttgttcg gtgcttggtg gtggccgtgg catctggaaa
     8341 tatggaaaag ttcaacaaaa agaaaagaga aaagaatgaa atcggatatc aagagttagt
     8401 tgagcggttt ctctagtttt ctgagtctca cctgcgacgg gaaggtcgcc gagccgggtg
     8461 gaatcgatcg ttgttgctcg gctttcatat cggtttggtt ggaagcggct gaaaacggaa
     8521 agaagtggaa gaaggaaaag agtgtggtgt gacaggaaaa tggtaattag agggtgccaa
     8581 ataaccagct atattttgtt tttttttgaa aacattttta aaaagaaaaa tacgataatg
     8641 atatcagatg gatttccgga aaactggtat gaaaaatttc aacctttttg agtacatgta
     8701 atcaaaatac actttgtaaa ttatcatttt tattgaaact ccaccatttt tctatttata
     8761 acgctaataa tttgaaaaag aaacctattg cgaaccgcgg ggtgaatccc aaaaacgaat
     8821 gcgttttggt ggagtgattg attcgaatcg aagaagaaaa agaagaagac gtggaataga
     8881 gagctcactc ttaaccgagc agcacacacc gacagaaaaa aaaatgaaat gaatgagggt
     8941 cttcttcttc ttcttcttcg aatgattgac agaaatggga aaaagaggaa gattgagaag
     9001 ggaaaaagga aggagaaaag aagcagaaga agacgtcaga gaggagagga acgagcggaa
     9061 aagcagcggg cgcaagtcat agaagtagca gagctgggga gaagaagaca ctatccaaga
     9121 aaggaatgac gagagagtat gcaaaggggt atagggtgca gacagaatag gaacagaata
     9181 acagatgatg agccaagaag agttgaaaag ggcgatgaat ttgtcatgta acttaatttg
     9241 ggtcaatttg agcatgatga attgaaatca tcccttgttg ggagttaata accggtttgt
     9301 tatcagaaac cctgtaatag aagggcgccc taactttgag ccaattcatc ccggtttctg
     9361 tcaaatatat caaaaagtgg tcaactgaca aattgttttt gatattataa taaacatttt
     9421 atccgttaac aattttcgaa tactttttac aaggacttgg ataaattggc tcaaagagcc
     9481 tgcttttttg tacaaacttg aattcaaaaa cgcagaactc gtactgttgg atgcctgtgt
     9541 agcggtcctc tattgatttt tgctattttt tgataatttt actgtaaatt atgagttatt
     9601 ccaattaaca ttcctagcta aatgtaagtt agcgaccaat tttttagcaa ccccatttta
     9661 tgacttttca gaatatcgcc tactgggtca acgacacttg tttggaacta agggtcttcc
     9721 caattttttt taaatcttca taggcttgac agaatcaggg ttcttcaagc agctttgaga
     9781 gggaatcaat tgcaacattt gatgttaaat ttatattttt tatagttctc tggtgaaatg
     9841 gggaccaccg caaagcccct tttatgtttt taacttgaat aaaatcttgt attaaattgt
     9901 gatttataga tctgtatggg taatttgaag gaaaagaaag ggagctccca aaagtgcctt
     9961 acagatccag gcccaataaa tgtaaactat tgggcagtag tatggcaaaa atcgtgcgga
    10021 atttgaaaaa cctagaatta aaataaatat tcaacacaaa tgttcgagat attttaagtt
    10081 cggagtgtat atctagaaca tattgtacaa gtaggtctac gggatcgtct acgggaaaat
    10141 ttgatttcct cgaagatgta tttaatgaat tctctaatgt tgcaggtctt tgtatttgac
    10201 agaatgttgg tttttgaaac ttattcgcca taattgcttg gctactacat gtctcatgcc
    10261 attgacacta tcctgtactc gaaatttgtt aagtaactac ccaccgtcca cttatgacga
    10321 gagggtcaat taaggtcact ccacaaaagt aaatttgaaa acacatgaga aaaagtgatc
    10381 tgaattccct tgaagtggct aaaacaaagc agatgaggga tacgacgtat gcgactactg
    10441 agagataagg tcgaaacttg ataaggatgc tgcttacctg cttagcaagt ttcttcgcac
    10501 aggagaaagt gacaagctaa actatcaaat tcatctaaaa cctcaaaaaa ttattttgga
    10561 tgaaataatg ttttattttg agcaacgcat aaggcaaaga ataaattaaa ggcgatccgc
    10621 ttagcggcga gttccgtcct cgaagaagat tcctccatcg atggcgcagt actttggaca
    10681 gattccacgc tctcctgggg ttcctggtgg tccgactggt cctggctgtc ctgggtttcc
    10741 gtcggctcct ggcttgccgt ctggtcctgg tggtcccttt ggtccaggtt gtcctgaaag
    10801 atgtggtgat tagatatgga ttgaaaaact atgaaatgat tacctggggt tccgtcgttt
    10861 cctggttgtc cggatggtcc tggtggtccg actggtcctg gctctccggt ggatcctggc
    10921 tctccctttt ggactggctc gcagacagct ggaagtccct ccaacttttc tatacaaagt
    10981 tgatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctggt
    11041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
    11101 gaacatgtga gcaaaaggcc a
//
