LOCUS       sequence-257157-        7708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 140_pAAV-ProA33-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2152..2161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2158..3084
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(3088..3090,3091..3093,3094..3807)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3088..3090,3091..3093,3094..3804)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3133..3154)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3394..3413)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3741..3762
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3835..4423
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3888..3908)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4306..4317)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4455..4931
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4654..4673)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4971..5111
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4971..5100
                     /label=AAV2 ITR
     rep_origin      5186..5641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5273..5292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5483..5504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5658..5677)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5777..5799
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5837..5855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5923..6027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6028..6096,6097..6888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6246..6265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7059..7647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7548..7567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgcccacct ttaagcccct ctctcaggcg gcagaggcaa
      181 gagtttgagg ccagcctggt ctacagaggg aatttcagga aagtcagggc tacacagaga
      241 aaccctgcct caaacaaaca aactaaaaag aatgactgtt cagagcaata agcgcccaaa
      301 gttggaaaaa aaattggatg aaaacttggt attccttaca ttgaatcttc cttaagttaa
      361 aagttaaaag tggggacttg agttgaaaca gcaagtttag agagtactat gtgctaggtc
      421 cacaactgcc acccttggtg gctttcttgt aaacttgcta ctagccagag tttctggaaa
      481 gaggaacctc gattgagaaa gtgcctccac cagattggct tgtagacaag gctgtgggtc
      541 attttcttgg ttaacattga cttgggaggg tcaagcccag tgttctctct gggcaggtcc
      601 tgggatactt tgaagaaggc agtgtgggta gacatgagat gctacccagt gaatagcatt
      661 cctctgtggc ctctgcttca gtttctacct tcgagtccct ccctgccttg gctctgacct
      721 ggaatatgta ggaaataaac cctttcctgt ccaagttgct ttttggtcag tctttatcac
      781 agcaatagaa agcagataat atactattta aatgccctaa attaggtaaa agcaatccaa
      841 tgtctgtagt tagatcactt tataaacaaa atatgcttca cacacacaca cacacacaca
      901 cacacacaca cactaaaata tcaatttcag atgggcagtg gtggagcaca cctttaatcc
      961 cagcccacgg gaggcagagg caggcagatc tctgagtttg gggtcagcct aatttacaga
     1021 gcaggttcta ggacagccaa ggctacacag agaaaccctg tcctgaaaaa caaaacaaat
     1081 atacatatgt gtgtgtacct atgcatgcat gtttttttat tagatgagaa cattttggaa
     1141 gtcagttgca ttcaaagtga ctagggcaag tactgctgaa ctgtgcactg gaaatggtca
     1201 attaaaaagg ctgactttat gctacatatg tattttatca ccatcaattt ttaaatatgc
     1261 atgatataat tgaaacccac taaattttgt ttttaaaatg tatatataca tgcaaaagat
     1321 gaatgaagga atccttgagg gccagctaat aagtgcatgc taaaactaag tcccaaaagt
     1381 caccctttga cctccatgca cacatgtgca cgcgtgtgcg cgcacgcaca cacatacaca
     1441 cacacacaca cacacacata ctttgtcaac taattaatga aaatatgcag ttctcagctt
     1501 ttatatgcta ttgttggtgt gctaaagttt tggaaagtcc tccaatgatg ccagatcagg
     1561 gagagtctgc ccacaagttt ttgtatattt tctcactgag gcatctattc agtttgggca
     1621 gcagacactg agcagaacgt agcacggcaa tgcttggtag caatgcctgt ccggccagca
     1681 ctcagaagac ggaggcagga gaatcatagc ttccagtcag cctcttctac aatatagtca
     1741 gttggaagtc agccagctta gacaacatgg agagcctgtg ccgaaagcca ctgggtaagc
     1801 ccgaatctca gtagcagaga gctgcccagg gtgcgtactg caaaaaaaaa acctcaaaca
     1861 acagaagtag ggaggtgtaa aataaagtgt aggggggtgg aatttaagct gatgtggact
     1921 tccaaataaa gttacctttt agatacctat ttaaatcaat agcatagacc tgaaactgtc
     1981 tatcagaaaa tgtgtctatt ctgaggaagg agtgctaacg aggttctgtg aggggggcct
     2041 ctggctttga gagggtgtac catcacataa gactcctaaa agcacatact tttataaatt
     2101 caccatgagc tttaacatct tctttgtcat ttcgcagact gagccggatc cgccaccatg
     2161 gattatggag gcgccctgag tgccgttggg cgcgagctgc tatttgtaac gaacccagta
     2221 gtcgtcaatg gctctgtact tgtgcctgag gaccagtgtt actgcgcggg ctggattgag
     2281 tcgcgtggca caaacggtgc ccaaacggcg tcgaacgtgc tgcaatggct tgctgctggc
     2341 ttctccatcc tactgcttat gttttacgcc taccaaacat ggaagtcaac ctgcggctgg
     2401 gaggagatct atgtgtgcgc tatcgagatg gtcaaggtga ttctcgagtt cttcttcgag
     2461 tttaagaacc cgtccatgct gtatctagcc acaggccacc gcgtccagtg gttgcgttac
     2521 gccgagtggc ttctcacctg cccggtcatt tgcattcacc tgtcaaacct gacgggcttg
     2581 tccaacgact acagcaggcg caccatgggt ctgcttgtgt ctgatattgg cacaattgtg
     2641 tggggcgcca cttccgccat ggccaccgga tacgtcaagg tcatcttctt ctgcctgggt
     2701 ctgtgttatg gtgctaacac gttctttcac gctgccaagg cctacatcga gggttaccac
     2761 accgtgccga agggccggtg tcgccaggtg gtgactggca tggcttggct cttcttcgta
     2821 tcatggggta tgttccccat cctgttcatc ctcggccccg agggcttcgg cgtcctgagc
     2881 gtgtacggct ccaccgtcgg ccacaccatc attgacctga tgtcgaagaa ctgctggggt
     2941 ctgctcggcc actacctgcg cgtgctgatc cacgagcata tcctcatcca cggcgacatt
     3001 cgcaagacca ccaaattgaa cattggtggc actgagattg aggtcgagac gctggtggag
     3061 gacgaggccg aggctggcgc ggtacccatg gtgagcaagg gcgaggagct gttcaccggg
     3121 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     3181 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     3241 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     3301 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     3361 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     3421 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     3481 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3541 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     3601 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     3661 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     3721 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     3781 ctcggcatgg acgagctgta caagtgataa gaattcgata tcaagcttat cgataatcaa
     3841 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3901 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3961 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4021 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4081 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4141 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4201 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctat
     4261 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4321 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4381 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgagcgctg
     4441 ctcgagagat ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg
     4501 aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt
     4561 ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc
     4621 aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag
     4681 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     4741 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     4801 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     4861 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     4921 tccctgtcct tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct
     4981 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     5041 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     5101 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     5161 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5221 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     5281 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5341 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5401 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5461 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     5521 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     5581 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     5641 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5701 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5761 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5821 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5881 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5941 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6001 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6061 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6121 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6181 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6241 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6301 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6361 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6421 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6481 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6541 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6601 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6661 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6721 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6781 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6841 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6901 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6961 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7021 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7081 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7141 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7201 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7261 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7321 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7381 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7441 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7501 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7561 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7621 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7681 gccttttgct ggccttttgc tcacatgt
//
