LOCUS       sequence-25719-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Saunders C, Ferrer JV, Shi L, Chen J, Merrill G, Lamb ME, 
            Leeb-Lundberg LM, Carvelli L, Javitch JA, Galli A
  TITLE     Amphetamine-induced loss of human dopamine transporter activity: an 
            internalization-dependent and cocaine-sensitive mechanism.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Jun 6. 97(12):6850-5.
  PUBMED    10823899
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Jun 6. 97(12):6850-5."
COMMENT     Sequence Label: pcihygroflagsynhDAT
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             118..141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct actagagaan ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accccgggtg tacaaggtta acggtaccgc caccatggac
      121 tacaaggacg atgacgataa ggccagcaag tccaaatgca gcgtgggact gatgtccagc
      181 gtggttgccc ccgctaagga gcccaacgcc gtgggcccca aggaggtgga actgattctc
      241 gtgaaggagc agaacggcgt gcagctcacc tccagcacac tgaccaaccc taggcagagc
      301 cccgtggagg cccaggaccg ggagacctgg ggcaagaaaa tcgacttcct gctcagcgtg
      361 atcggcttcg ccgtcgacct ggctaacgtt tggcggttcc cctacctgtg ctacaagaac
      421 ggcggangcg ctttcctggt gccctacctc ctctttatgg tgatcgctgg catgcccctg
      481 ttctacatgg agctggnccn nnnnncnnnt taancnggan ggagccgcan gtgtgtggaa
      541 ganctgcccc atcctcaaag gcgtgggatt caccgtgatc ctgatcagcc tgtacgtang
      601 cttcttttac aacgtcatca ttgcctgggc tctccactac ctgttctcga gcttcaccac
      661 agagctgccc tggatccact gcaacaatag ctggaacagc cccaactgca ncgatgcgca
      721 ccccggcgac agctnccgan acagctcngc ntgaacnaca ccttngnaac cacacccgcc
      781 gctgaatact tcgaacgggg cgtgctgca
//
