LOCUS       sequence-257191-        9720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into 
            delta-sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Zhao EM, Suek N, Wilson MZ, Dine E, Pannucci NL, Gitai Z, Avalos JL,
            Toettcher JE
  TITLE     Light-based control of metabolic flux through assembly of synthetic 
            organelles.
  JOURNAL   Nat Chem Biol. 2019 Jun;15(6):589-597. doi: 
            10.1038/s41589-019-0284-8. Epub 2019 May 13.
  PUBMED    31086330
REFERENCE   2  (bases 1 to 9720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0284-8"; journalName: "Nat Chem Biol"; date: 
            "2019-06"; volume: "15"; issue: "6"; pages: "589-597"
COMMENT     Sequence Label: pNS3
FEATURES             Location/Qualifiers
     source          1..9720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..705
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1112..1123
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1383..2078
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     CDS             2091..3584
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     primer_bind     complement(3638..3654)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3639..3655)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4198..4217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4199..4217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4224..4241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4224..4240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4233..4255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4381..4836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4468..4487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4678..4699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5192..5211)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5311..5333
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5371..5389)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5473..5577
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5578..5646,5647..6438)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5796..5815)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6609..7197
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7098..7117
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7351..7368
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7485..7506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7521..7526,7527..7544,7545..7551)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7559..7575
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7564..7586
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7583..7599
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7583..7599
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7620..7638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(7846..7879)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            complement(7931..8851)
                     /label=bleMX6
                     /note="yeast selectable marker conferring phleomycin 
                     resistance"
     terminator      complement(7931..8127)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(8133..8507)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(8508..8851)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    complement(8906..8939)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca
       61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga
      121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt
      181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc
      241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata
      301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca
      361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca
      421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt
      481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc
      541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac
      601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc
      661 tttttctgca caatatttca agctatacca agcatacaat caactccatt gcggccgcta
      721 aaatcatggc tagcgcctca aacgattata cccaacaagc aacccaaagc tatggggcct
      781 accccaccca gcccgggcag ggctattccc agcagagcag tcagccctac ggacagcaga
      841 gttacagtgg ttatagccag tccacggaca cttcaggcta tggccagagc agctattctt
      901 cttatggcca gagccagaac acaggctatg gaactcagtc aactccccag ggatatggct
      961 cgactggcgg ctatggcagt agccagagct cccaatcgtc ttacgggcag cagtcctcct
     1021 atcctggcta tggccagcag ccagctccca gcagcacctc gggaagttac ggtagcagtt
     1081 ctcagagcag cagctatggg cagccccaga gtgggagcta cagccagcag cctagctatg
     1141 gtggacagca gcaaagctat ggacagcagc aaagctataa tccccctcag ggctatggac
     1201 agcagaacca gtacaacagc agcagtggtg gtggaggtgg aggtggaggt ggaggtaact
     1261 atggccaaga tcaatcctcc atgagtagtg gtggtggcag tggtggcggt tatggcaatc
     1321 aagaccagag tggtggaggt ggcagcggtg gctatggaca gcaggaccgt ggaggtggag
     1381 gaatggtgag cgagctgatt aaggagaaca tgcccatgaa gctgtacatg gagggcaccg
     1441 tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     1501 agaccatgag aatcaaggtc gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     1561 ctaccagctt catgtacggc agcagaacct tcatcaagca ccctccgggc atccccgact
     1621 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     1681 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     1741 tcaaggttag aggggtgaac ttcccagcca acggccctgt gatgcagaag aaaacactcg
     1801 gctgggaggc ctccaccgag acgatgtacc ccgctgacgg cggcctggaa ggcgcatgtg
     1861 acatggccct gaagctcgtg ggcgggggcc acctgatctg caaccttgag accacataca
     1921 gatccaagaa acccgctacg aacctcaaga tgcccggcgt ctacaacgtg gaccacagac
     1981 tggaaagaat caaggaggcc gacgatgaga cctacgtcga gcagcacgag gtggctgtgg
     2041 ccagatactc tactggtggc gctggtgatg gaggtaaagc ggccacgcgt atgaagatgg
     2101 acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag
     2161 cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag
     2221 gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat
     2281 ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt
     2341 cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct
     2401 atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga
     2461 tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa
     2521 agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat atgtcgattg
     2581 aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga
     2641 tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg
     2701 cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt
     2761 tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta
     2821 cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt
     2881 gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg
     2941 cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact
     3001 tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg
     3061 atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg
     3121 gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa
     3181 gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata
     3241 cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta
     3301 tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg
     3361 acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat
     3421 ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg
     3481 gaacaaacta tgcgaaaccc attgtggaca tcgacacagc tcgtgagcta ctagctaaag
     3541 ctatttcaag aacccgtgga gcacagatca tgatcggagc agcagcccgg gatccaccgg
     3601 tcgccaccgg gccctaagaa ttcgatatca agcttatcga taccgtcgac ctcgagtaat
     3661 gatcatctct gcttttgtgc gcgtatgttt atgtatgtac ctctctctct atttctattt
     3721 ttaaaccacc ctctcaataa aataaaaata ataaagtatt tttaaggaaa agacgtgttt
     3781 aagcactgac tttatctact ttttgtacgt tttcattgat ataatgtgtt ttgtctctcc
     3841 cttttctacg aaaatttcaa aaattgacca aaaaaaggaa tatatatacg aaaaactatt
     3901 atatttatat atcatagtgt tgataaaaaa tgtttatcca ttggaccgtg tatcatattt
     3961 atcgccggcg aataaattaa ggcgcgccaa catatccgga ggtaccataa aacggaatga
     4021 ggaataatcg taatattagt atgtagaaat atagattcca ttttgaggat tcctatatcc
     4081 tcgaggagaa cttctagtat attctgtata cctaatatta tagcctttat caacaatgga
     4141 atcccaacaa ttatctcaac attcacccat ttctcagttt aaacggccac gctggccccc
     4201 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4261 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4321 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4381 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4441 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4501 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4561 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4621 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4681 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4741 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4801 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     4861 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
     4921 taatttgtga gtttagtatt aggatctcta cccttggcga aaagtcctct gccaacaatg
     4981 atgatatctg atccaccact tacaacttcg tcgagggttc tgtactgctg acccaatatg
     5041 cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt
     5101 accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat
     5161 gaattgaaaa ggtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5221 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5281 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5341 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5401 atgtcatgat aataatggtt tcttagacgt cacgcgccga ggacgtcagg tggcactttt
     5461 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5521 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5581 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5641 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5701 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5761 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5821 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5881 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5941 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6001 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6061 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6121 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6181 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6241 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6301 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6361 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6421 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6481 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6541 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6661 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6721 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6781 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6841 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6901 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6961 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7021 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7081 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7141 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7201 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7261 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7321 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7381 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7441 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7501 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7561 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagctcgga
     7621 attaaccctc actaaaggga acaaaagctg ggccggccat ttaaatgttt aaacaacctg
     7681 ttggaataga aatcaactat catctactaa ctagtattta cattactagt atattatcat
     7741 atacggtgtt agaagatgac gcaaatgatg agaaatagtc atctaaatta gtggaagctg
     7801 aaacgcaagg attgataatg taataggatc aatgaatata aacattaccg ttcgtatagc
     7861 atacattata cgaagttata ttaagggttc tcgagagctc gggatggcgg cgttagtatc
     7921 gaatcgacag cagtatagcg accagcattc acatacgatt gacgcatgat attactttct
     7981 gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg aaaaaaatat aaatcacgct
     8041 aacatttgat taaaatagaa caactacaat ataaaaaaac tatacaaatg tcaagttctt
     8101 gaaaacaaga atctttttat tgtcagtact gatcagtcct gctcctcggc cacgaagtgc
     8161 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
     8221 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
     8281 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
     8341 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
     8401 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
     8461 acgtcgcgcg cggtgagcac cggaacggcg ctggtcaact tggccatggt tgtttatgtt
     8521 cggatgtgat gtgagaactg tatcctagca agattttaaa aggaagtata tgaaagaaga
     8581 acctcagtgg caaatcctaa ccttttatat ttctctacag gggcgcggcg tggggacaat
     8641 tcaacgcgtc tgtgagggga gcgtttccct gctcgcaggt ctgcagcgag gagccgtaat
     8701 ttttgcttcg cgccgtgcgg ccatcaaaat gtatggatgc aaatgattat acatggggat
     8761 gtatgggcta aatgtacggg cgacagtcac atcatgcccc tgagctgcgc acgtcaagac
     8821 tgtcaaggag ggtattctgg gcctccatgt cgctggccgg gtgacccggc ggggacgagg
     8881 caagctaaac agatctctag acctaataac ttcgtatagc atacattata cgaacggtac
     8941 ccgggaaaca gctgcgccgg gaaacagctg gccgggatcg aagaaatgat ggtaaatgaa
     9001 ataggaaatc aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag
     9061 tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat
     9121 tgcacaatca tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg
     9181 ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg
     9241 ctaaggggcg agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc
     9301 acgacaactg gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag
     9361 tatactagaa gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata
     9421 gagcgaccat gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa
     9481 cagcaatagg gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt
     9541 ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag
     9601 ggaactttac acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg
     9661 ggggtaacac ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat
//
