LOCUS       sequence-257192-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPGK1_FUSN_Citrine _PixE_TCYC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Zhao EM, Suek N, Wilson MZ, Dine E, Pannucci NL, Gitai Z, Avalos JL,
            Toettcher JE
  TITLE     Light-based control of metabolic flux through assembly of synthetic 
            organelles.
  JOURNAL   Nat Chem Biol. 2019 Jun;15(6):589-597. doi: 
            10.1038/s41589-019-0284-8. Epub 2019 May 13.
  PUBMED    31086330
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-019-0284-8"; journalName: "Nat Chem Biol"; date: 
            "2019-06"; volume: "15"; issue: "6"; pages: "589-597"
COMMENT     Sequence Label: EZ-L498
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             381..392
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             join(652..654,655..657,658..1368)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(697..718)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(958..977)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1305..1326
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2542..2789
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2558..2576)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(4256..4443)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        complement(4769..4787)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4808..4824)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4808..4824)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4821..4843)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4832..4848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4856..4862,4863..4880,4881..4886))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4901..4922
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5039..5056)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5210..5798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5290..5309)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5969..6760,6761..6829))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6592..6611
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6830..6934)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7018..7036
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7074..7096)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7196..7215
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7571..8026)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7708..7729)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7920..7939
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8152..8174
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8166..8183
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8167..8183
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8190..8209
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8190..8208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atggcctcaa acgattatac ccaacaagca acccaaagct atggggccta ccccacccag
       61 cccgggcagg gctattccca gcagagcagt cagccctacg gacagcagag ttacagtggt
      121 tatagccagt ccacggacac ttcaggctat ggccagagca gctattcttc ttatggccag
      181 agccagaaca caggctatgg aactcagtca actccccagg gatatggctc gactggcggc
      241 tatggcagta gccagagctc ccaatcgtct tacgggcagc agtcctccta tcctggctat
      301 ggccagcagc cagctcccag cagcacctcg ggaagttacg gtagcagttc tcagagcagc
      361 agctatgggc agccccagag tgggagctac agccagcagc ctagctatgg tggacagcag
      421 caaagctatg gacagcagca aagctataat ccccctcagg gctatggaca gcagaaccag
      481 tacaacagca gcagtggtgg tggaggtgga ggtggaggtg gaggtaacta tggccaagat
      541 caatcctcca tgagtagtgg tggtggcagt ggtggcggtt atggcaatca agaccagagt
      601 ggtggaggtg gcagcggtgg ctatggacag caggaccgtg gaggtggagg aatggtgagc
      661 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
      721 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
      781 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
      841 accttcggct acggcctgat gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
      901 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
      961 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     1021 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     1081 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     1141 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     1201 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     1261 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     1321 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggc ggccacgcgt
     1381 atgagcaatt cagttttgtc cacttcttcc ccaggtaata tccaaactcg gcaggcgatt
     1441 ccggcccaac tcctctattc cctcgtcaac caaaaagcca gcggcaaact tgtggtacaa
     1501 aacccctttg acgaatttgt ttattggcaa gtttatttgg gggatggtcg tattcacttg
     1561 gccaacagcg ccaatggttg ttccgaaaga cttaattatc tgattggtag cacccttcgt
     1621 cagaagggca ttgaactgcc atcaagaatt gctgacgatt atatttttct ttccgggctc
     1681 tggaaaaggg atgttttttc ctttcagcaa acccgctcga ttctgaccca agctagccaa
     1741 gaagccttag ttcaggtact ttccttacct aaaacatcct atcgttttaa tcaagaaaaa
     1801 gaactgagcg aactattttt gaatttagag ttagaaaaaa ctattacccc catcaaacat
     1861 aaaattcgct attggtggga attgaggtcc tatattaact cgcctttcca gcgaccccta
     1921 gtggaaaact ggcttagtct gcgccaaaat ttggtgaaaa ataacctcca tggccaacat
     1981 tggctaaaac gcttccacac ttgcttggaa aatctaaatt gcttatatga aattgccggg
     2041 catttggaaa tcagtacttt ggagttggca ttaatgctac agcccatggt caagactggc
     2101 gaaatcaaga tgctgcctta tcaagaaatt caaacggatg accgcccttt ggtggcttat
     2161 gttgaccata gggaagtcca gcagcgcatg atgacctatt ctttggaaaa agaaggctat
     2221 cgagccttag tactggatga tccctttcgg gctttaacgg tgttgttgaa ttctcccccc
     2281 aatctgatta tcataaatac tgacttgccg gacatgaacg gttatcaact ctgttcgttt
     2341 tgccgtaagt ccaatcaact taaagatgtg ccgatcctac tattggggaa aggtgacaat
     2401 ttgatcagcg gtattcgtat caaactttgt caagcttctg ggtacttaag tgagcctttt
     2461 gttccagaag aattactcga tcgggtggca aaactccttc ccaccaataa aaccaactcc
     2521 tgagtcgacc tcgagtaatg atcatgtaat tagttatgtc acgcttacat tcacgccctc
     2581 cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc taggtcccta
     2641 tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa atttttcttt
     2701 tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg cttgagaagg
     2761 ttttgggacg ctcgaaggct ttaatttgcg gccttatcgc cggcgaataa attaaggcgc
     2821 gccaacatat ccggaggtac ccaaagccga atccaccacg gtcaccgttg tttctgaaga
     2881 aaccaccacg gccgccaccg ttgttgaaac caccacgagg agcagaatcg cctcttctgc
     2941 caccaccgtt acggatgtga ggtggaacgt aaccaccacg tctttgttgc ccttcaggca
     3001 tggaaacttg ctctgctaac tcagtcatat tggatatatg tattactttc tttatttttt
     3061 gtagtttagt cttctatttc ttttatagtt tggtatcttt ttgcaaaaaa tttacctcta
     3121 tatcgtgttc gtgcgtattt cttcctctat aatatgaaaa gaaaggcagt agtcttggtt
     3181 ttctattatt ccaaatatta caagaatacc cagaaccaca agaagcagaa gaaactaact
     3241 gctttatata cttaatttcc cgccacaagc tcatcgccca gaagtttttt tcagtttttc
     3301 acctgacaat ctggcagctc gctgaaaaat tttcacctgg ggtcgcctgc tacgtagagt
     3361 gatcacgttt atgaatagca cattatatag tggcccagac ggtctgatgg tagttcatgt
     3421 agttaagaat aaatactctg tttgtgtgat ttctatgtgt acgttatcta tataaatatt
     3481 gttgatggtg gtagctgtgg gctgtgttct atgctaggac acccttagtg gatggaacgt
     3541 catcgcgacc cgtaggtgtt cttgcctctc tgatggcgat ggcgagcgcc ttgaaagctg
     3601 attcggagcg gtggtggtcg ttggtgcccc gcagacagtc cacatgcatg gtgatacgcg
     3661 cctcagtggc gaaactctcc aagaagtgcg ggatcatctc tgtggatagc tggcctatgc
     3721 gttctcgctt caggcccagc tccaccacgg cgaaaggcct attggagaag tcaaccacag
     3781 cgcggctcag cgcctcgtcc agtggtgcaa acccatgccc gaacctcttg ataccacgca
     3841 cggagccaag cgcctccttg aacgcttggc ccagcgcaat cccacagtcc tcaacagtgt
     3901 ggtggtcatc gatgtgcaag tcccctatac actccaggat cagagaccac ccgccatgct
     3961 tcgccagcgc agtcaacatg tggtccaaaa acccaactcc agtgtgcaca tggatggtct
     4021 gcccatcacc actagcatat tccttgccca gtatgctctc ccgtacagaa acagacccac
     4081 cgtcaagatc cagcgccagc tgtatattcg tctcctgcgt aaccctctta acaaacgcca
     4141 tcctttgctc gatgcttctc tttgcttcaa ttatctcaac tttaagtaaa cctgaagtaa
     4201 actagaacta gattaaatca gaaggcatcc gcagctttat atacatatag agcctctttg
     4261 ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc acattacttt
     4321 atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa agagtcatcc
     4381 gctaggtgga aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata tagagtgtac
     4441 tagaggaggc caagagtaat agaaaaagaa aattgcggga aaggactgtg ttatgacttc
     4501 cctgactaat gccgtgttca aacgatacct ggcagtgact cctagcgctc accaagctct
     4561 taaaacgaga attaagaaaa agtcgtcatc tttcgataag tttttcccac agcaaagcaa
     4621 tagtagaaaa aaacaatggg aaacgttgaa tgaagacaaa gcgtcgtggt ttaaaaggaa
     4681 atacgctcac gtacatgcta gggaacagga ccgtgcagcg gccagcgtgg ccgtttaaac
     4741 atttaaatgg ccggcccagc ttttgttccc tttagtgagg gttaattccg agcttggcgt
     4801 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4861 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4921 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4981 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5041 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5101 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5401 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5641 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5881 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5941 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6001 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6061 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6121 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6181 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6241 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6301 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6361 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6421 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6481 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6541 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6601 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6661 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6721 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6781 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6841 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6901 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6961 acgtcctcgg cgcgtgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     7021 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     7081 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     7141 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     7201 tcagagcaga ttgtactgag agtgcaccat accacctttt caattcatca tttttttttt
     7261 attctttttt ttgatttcgg tttccttgaa atttttttga ttcggtaatc tccgaacaga
     7321 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata ttgggtcagc
     7381 agtacagaac cctcgacgaa gttgtaagtg gtggatcaga tatcatcatt gttggcagag
     7441 gacttttcgc caagggtaga gatcctaata ctaaactcac aaattagagc ttcaatttaa
     7501 ttatatcagt tattacccta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7561 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7621 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7681 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7741 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7801 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7861 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7921 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7981 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     8041 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8101 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8161 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggg gccagcgtgg
     8221 ccgtttaaac atttaaatgg ccggccgctg ccaccgttgt ttccaccgaa gaaaccaccg
     8281 ttgccgtaac caccacgacg gttgttgcta aagaagctgc caccgccacg gccaccgttg
     8341 tagccgccgt tgttgttatt gtagttgcta ctgttatttc tggcacttct tggttttcct
     8401 cttaagtgag gaggaacata accattctcg ttgttgtcgt tgatgcttaa attttgcact
     8461 tgttcgctca gttcagccat aatatgaaat gcttttcttg ttgttcttac ggaataccac
     8521 ttgccaccta tcaccacaac taactttttc ccgttcctcc atctctttta tatttttttt
     8581 ctggagttca agagaaaaaa aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt
     8641 cagaatgaca cgtatagaat gatgcattac cttgtcatct tcagtatcat actgttcgta
     8701 tacatactta ctgacattca taggtataca tatatacaca tgtatatata tcgtatgctg
     8761 cagctttaaa taatcggtgt caagatctga gctccaccgc ggtggcggcc gcttatttac
     8821 tcaggatccc gcatgttgca ggtgggagtt ccagctcccg ggaaacagct gcaagaatta
     8881 ctcgtgagta aggaaagagt gaggaactat cgcatacctg catttaaaga tgccgatttg
     8941 ggcgcgaatc ctttattttg gcttcaccct catactatta tcagggccag aaaaaggaag
     9001 tgtttccctc cttcttgaat tgatgttacc ctcataaagc acgtggcctc ttatcgagaa
     9061 agaaattacc gtcgctcgtg atttgtttgc aaaaagaaca aaactgaaaa aacccagaca
     9121 cgctcgactt cctgtcttcc tattgattgc agcttccaat ttcgtcacac aacaaggtcc
     9181 tagcgacggc tcacaggttt tgtaacaagc aatcgaaggt tctggaatgg cgggaaaggg
     9241 tttagtacca catgctatga tgcccactgt gatctccaga gcaaagttcg ttcgatcgta
     9301 ctgttactct ctctctttca aacagaattg tccgaatcgt gtgacaacaa cagcctgttc
     9361 tcacacactc ttttcttcta accaaggggg tggtttagtt tagtagaacc tcgtgaaact
     9421 tacatttaca tatatataaa cttgcataaa ttggtcaatg caagaaatac atatttggtc
     9481 ttttctaatt cgtagttttt caagttctta gatgctttct ttttctcttt tttacagatc
     9541 atcaaggaag taattatcta ctttttacaa caaatataaa acaccgcggt ggcggccgct
     9601 aaaatc
//
