LOCUS       sequence-257211-        5262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PIq driving expression LacI-T, the Lactose inducible 
            chimera with the TAN DBD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[Iq:LacI-T]
FEATURES             Location/Qualifiers
     source          1..5262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..62
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        127..204
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(224..243)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1535..1766
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      1801..1887
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2425..3375)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3423..3645
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4137..4231)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4261..5052,5053..5121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4884..4903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5122..5213)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctagcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
       61 gccctttcgt cttcacctcg acaattcgcg cgcgaaggcg aagcggcatg catttacgtt
      121 ggtaccgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
      181 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      241 gccggtgtct ctacggcgac cgtttccaac gtggtgaacc aggccagcca cgtttctgcg
      301 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      361 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      421 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      481 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      541 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      601 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      661 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      721 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      781 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      841 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      901 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      961 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     1021 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
     1081 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1141 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1201 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtgaccatgg
     1261 gcgcaacgca attaatgtaa gttagcgcga attgtcgagg gaaatcataa aaaatttatt
     1321 tgctttcagg aaaatttttc tgtataatag attcataaat ttgagagaag agtttaaata
     1381 tggctggttc tcgcagaaag aaacatatcc atgaaatccc gccccgaatt catatgtcta
     1441 gatagctgag tagcctagag ctagagtgcg cgacaggttt gatgacaaaa aattagcgca
     1501 agaagacaaa aatcaccttg cgctaatgct ctgttacagg tcactaatac catctaagta
     1561 gttgattcat agtgactgca tatgttgtgt tttacagtat tatgtagtct gttttttatg
     1621 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttttt
     1681 atactaagtt ggcattataa aaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1741 tatcagtcaa aataaaatca ttatttgatt tcaattttgt cccactcccc ctagaggcat
     1801 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1861 gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctagggtac gggttttgct
     1921 gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc
     1981 agccggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
     2041 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
     2101 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
     2161 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
     2221 tctgttacat tgtcgatctg ttcatggtga acagctttga atgcaccaaa aactcgtaaa
     2281 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
     2341 tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     2401 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     2461 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg
     2521 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     2581 gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct gatgtaatgg ttgttggtat
     2641 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     2701 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     2761 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     2821 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     2881 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     2941 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
     3001 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     3061 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     3121 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     3181 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     3241 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     3301 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     3361 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     3421 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
     3481 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     3541 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     3601 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     3661 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     3721 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
     3781 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
     3841 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
     3901 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
     3961 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
     4021 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
     4081 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
     4141 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     4201 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt ggtctgacag
     4261 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4321 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4381 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4441 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4501 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4561 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4621 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4681 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4741 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4801 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4861 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4921 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4981 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     5041 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     5101 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     5161 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggc
     5221 tcgagttccg cgcacatttc cccgaaaagt gccacctgac gt
//
