LOCUS       sequence-25722-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F
  TITLE     Point mutations in TOR confer Rheb-independent growth in fission 
            yeast and nutrient-independent mammalian TOR signaling in mammalian 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3514-9.
  PUBMED    17360675
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 27. 104(9):3514-9."
COMMENT     Sequence Label: pcDNA3-AU1-mTOR-E2419K
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             596..609
                     /estimated_length=14
     gap             613..622
                     /estimated_length=10
     gap             627..636
                     /estimated_length=10
     gap             639..649
                     /estimated_length=11
ORIGIN
        1 agctctctgg ctaactagag aanccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ctcgagccac catggacacg tacaggtaca tccttgggac
      121 aggccctgcc acggccaccg ccggtgccgc cacatccagc aacgtgagcg ttctgcagca
      181 gttcgccagt gggctgaaga gccggaatga ggagaccagg gccaaagcag ccaaggagct
      241 ccagcactat gtcaccatgg aacttcgaga gatgagtcag gaggagtcta ctcgcttcta
      301 tgaccagctg aaccatcaca tttttgaact ggtttccagc tcagacgcca atgagaggaa
      361 gggtggcatc ttggccattg ccagcctcat tggagtggaa ggtgggaatt ccaccaggat
      421 tggcagattt gccaactacc ttcggaacct cctcccctca agtgatccag ttgtcatgga
      481 gatggcatcc aaggccattg gccgccttgc aatggcaggg gacactttca ctgctgagta
      541 tgtggagttt gaagtgaagc gagccttgga gtggctgggt gctgaccgaa atgngnnnnn
      601 nnnnnnnnng cannnnnnnn nncnttnnnn nnnnnnggnn nnnnnnnnnc cncnccttnt
      661 tnttnnttcc agcaagttca gcccttcntt tgannannnn ttntnnnngc tnnnnngggn
      721 acccccaagc aggccatccg nngaaggagc tgnnggctgc cct
//
