LOCUS       sequence-257254-        6016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein mKate(KPS12) variant for optimized expression in
            Bacillus mycoides; under control of mannose inducible promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Yi Y, Frenzel E, Spoelder J, Elzenga JTM, van Elsas JD, Kuipers OP
  TITLE     Optimized fluorescent proteins for the rhizosphere-associated 
            bacterium Bacillus mycoides with endophytic and biocontrol agent 
            potential.
  JOURNAL   Environ Microbiol Rep. 2018 Feb;10(1):57-74. doi: 
            10.1111/1758-2229.12607. Epub 2018 Jan 5.
  PUBMED    29195004
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/1758-2229.12607"; journalName: "Environ Microbiol Rep"; 
            date: "2018-02"; volume: "10"; issue: "1"; pages: "57-74"
COMMENT     Sequence Label: pAD-KPS12-Pman
FEATURES             Location/Qualifiers
     source          1..6016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(980..999)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    3866..4006
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4021..4038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4192..4780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4272..4291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4951..5742,5743..5811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5574..5593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5812..5916)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaattcgcag ggattattcc ttgctttttt tgttataggg aaaaatgcct ttattaccgg
       61 aacctatggt aaaaaaagcg attttaatga gctgatttcg gtatacagtt gagacatcta
      121 gaggaaaatt aatgtcagaa cttatcaagg aaaatatgca catgaaattg tacatggaag
      181 gaacagtaaa taatcaccac tttaaatgta cctcagaagg agaaggaaaa ccatatgaag
      241 gtactcaaac catgcgtatt aaggccgttg aaggtggacc attgcctttt gcctttgata
      301 ttcttgctac atcttttatg tacggatcaa agacttttat caatcatacc caaggtatcc
      361 cagatttctt taaacagtca tttcctgaag gatttacatg ggaacgtgtc acaacttatg
      421 aagatggtgg agtattgaca gcaactcaag atacatcttt gcaagatggt tgtcttatct
      481 acaatgtaaa gatccgtgga gttaattttc caagtaatgg tcctgttatg cagaaaaaga
      541 cccttggatg ggaagcatct accgaaacat tatatcctgc cgatggtgga ttggaaggtc
      601 gtgctgatat ggcattgaaa cttgtcggtg gaggtcacct tatctgtaat ttgaagacca
      661 cataccgttc taaaaagcca gctaagaatc ttaagatgcc tggtgtttac tacgtcgatc
      721 gtcgtttaga acgtatcaaa gaagcagata aggaaactta tgttgaacag cacgaagtag
      781 ccgtcgcacg ttattgtgat ttgcctagta aattgggaca ccgttaataa taaaagcttg
      841 agtaggcatg caagcttgag taggacaaat ccgccgagct tcgacgagat tttcaggagc
      901 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
      961 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     1021 cgttcagaac aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa
     1081 acatgcagga attgacgatt taaacaatat tagctttgaa caattcttat ctcttttcaa
     1141 tagctataaa ttatttaata agtaagttaa gggatgcata aactgcatcc cttaacttgt
     1201 ttttcgtgtg cctatttttt gtgaatcgct aagaaaccat tattatcatg acattaacct
     1261 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     1321 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     1381 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     1441 atgcggcatc agagcagatt gtactgagag tgcaccatac aaaacatatt tcaacacaat
     1501 acaaatgggt tagttaaaaa agcaggcctt ctaaaggtct gcttttttta tttgattatg
     1561 taatttttaa tgccaggatg ccaataagcc ataacctcaa atgcaccatt tgcaacctcg
     1621 tcatcttctt cctcaatctt gaccagatcg ccgtcctccg catcaccaag attcagctct
     1681 ttatgtatct ccttcaaaat gccaccgtat ccaattaacc ttcgagctgc caacgcatca
     1741 tccaagtaaa gcaccgtgtt cagattgtct tcagtcacct tattaccgcg cacacaatcc
     1801 gtatccttaa ccggatattt agagatttcg cgaacagctt tttgctccat cattgcgttc
     1861 cgcacatcgt tttcaatctg ttcagcgtca atcttagctt tacctttcac tcgacgaata
     1921 tcgacaattg gagtgtaatc caatttcatc gcctttttcc aaaggctcgt ccactccgcc
     1981 tgcttaatat agtttttccc aaaataattt ttccttactg gtatcaacac atgaaaatga
     2041 ggatgatatg tatcttcttc atgatttttg gtaatctcta aagctctgaa aaatccaaga
     2101 accgaagttt ttactttttt gtactggaac agtttcctaa agccttccat catcgcagaa
     2161 atttgtggct tcagccgttc tccctttaca tttcgaatcg tcagcgtgag aaaaatccat
     2221 ccgcagccgt actgtctatt ggcttcctct acgatcaact tattgtgata agcaattttt
     2281 aacgacctgc gccacgcaca catcggacat aacctcactt tacaaaaatg ggcttgatac
     2341 agttttaact tgcccgtctc cgggtctctc ttaaacgaaa gatactctgc acaactaatt
     2401 agtttttcag cctttttgcc atagtaaggt gccccaatct tactctctaa cgcttcgtaa
     2461 tgctccgcca tgaggttcgt ccgtctcttt ttccccttcc aatcccgctt tttacctgtt
     2521 gcggttttat cttcgaggat gctataatca ttttcagatg aataaatcaa caaaaaaact
     2581 ccttctgagc tagttctcta gcattctatt attttgattc gacaccttaa taatagcaga
     2641 aggagttttt acctgtcaaa gaaccatcaa acccttgata cacaaggctt tgacctaatt
     2701 ttgaaaaatg atgttgtttc tatatagtat caagataaga aagaaaagga tttttcgcta
     2761 cgctcaaatc ctttaaaaaa acacaaaaga ccacattttt taatgtggtc tttattcttc
     2821 aactaaagca cccattagtt caacaaacga aaattggata aagtgggata tttttaaaat
     2881 atatatttat gttacagtaa tattgacttt taaaaaagga ttgattctaa tgaagaaagc
     2941 agacaagtaa gcctcctaaa ttcactttag ataaaaattt aggaggcata tcaaatgaac
     3001 tttaataaaa ttgatttaga caattggaag agaaaagaga tatttaatca ttatttgaac
     3061 caacaaacga cttttagtat aaccacagaa attgatatta gtgttttata ccgaaacata
     3121 aaacaagaag gatataaatt ttaccctgca tttattttct tagtgacaag ggtgataaac
     3181 tcaaatacag cttttagaac tggttacaat agcgacggag agttaggtta ttgggataag
     3241 ttagagccac tttatacaat ttttgatggt gtatctaaaa cattctctgg tatttggact
     3301 cctgtaaaga atgacttcaa agagttttat gatttatacc tttctgatgt agagaaatat
     3361 aatggttcgg ggaaattgtt tcccaaaaca cctatacctg aaaatgcttt ttctctttct
     3421 attattccat ggacttcatt tactgggttt aacttaaata tcaataataa tagtaattac
     3481 cttctaccca ttattacagc aggaaaattc attaataaag gtaattcaat atatttaccg
     3541 ctatctttac aggtacatca ttctgtttgt gatggttatc atgcaggatt gtttatgaac
     3601 tctattcagg aattgtcaga taggcctaat gactggcttt tataatatga gataatgccg
     3661 actgtacttt ttacagtcgg ttttctaatg tcactaacct gccccgttag tcgccattcg
     3721 ccagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3781 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3841 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     3901 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     3961 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     4021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4381 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4621 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4921 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4981 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5041 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5101 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5161 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5221 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     5281 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5341 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5401 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5461 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5521 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     5581 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5641 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5701 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5761 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5821 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5881 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5941 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     6001 gccctttcgt cttcaa
//
