LOCUS       sequence-25726-e         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F
  TITLE     Point mutations in TOR confer Rheb-independent growth in fission 
            yeast and nutrient-independent mammalian TOR signaling in mammalian 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3514-9.
  PUBMED    17360675
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 27. 104(9):3514-9."
COMMENT     Sequence Label: pcDNA3-FLAG-Rheb
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     gap             635..644
                     /estimated_length=10
     gap             692..706
                     /estimated_length=15
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctggaattca ccatggatta caaggatgac gatgacaagc cgcagtccaa gtcccggaag
      181 atcgcgatcc tgggctaccg gtctgtgggg aaatcctcat tgacgattca atttgttgaa
      241 ggccaatttg tggactccta cgatccaacc atagaaaaca cttttacaaa gttgatcaca
      301 gtaaatggac aagaatatca tcttcaactt gtagacacag ccgggcaaga tgaatattct
      361 atctttcctc agacatactc catagatatt aatggctata ttcttgtgta ttctgttaca
      421 tcaatcaaaa gttttgaagt gattaaagtt atccatggca aattgttgga tatggtgggg
      481 aaagtacaaa tacctattat gttggttggg aataagaaag acctgcatat ggaaagggtg
      541 atcagttatg aagaagggaa agctttggca gaatcttgga atgcagcttt tttggaatct
      601 tctgctaaag aaaatcagac tgctgtggat gnntnnnnnn nnnntnnttt nngnnnnnna
      661 aaaaaannnn gacggggnnn nnnnaggnng gnnnnnnnnn nnnnnnctcg gtgatgtgag
      721 cggcccgctc gagcatgc
//
