LOCUS       sequence-25727-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Urano J, Sato T, Matsuo T, Otsubo Y, Yamamoto M, Tamanoi F
  TITLE     Point mutations in TOR confer Rheb-independent growth in fission 
            yeast and nutrient-independent mammalian TOR signaling in mammalian 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27. 104(9):3514-9.
  PUBMED    17360675
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2007 Feb 27. 104(9):3514-9."
COMMENT     Sequence Label: pcDNA3-FLAG-Rheb-N153T
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             135..158
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
      121 ctgaattcac catggattac aaggatgacg atgacaagcc gcagtccaag tcccggaaga
      181 tcgcgatcct gggctaccgg tctgtgggga aatcctcatt gacgattcaa tttgttgaag
      241 gccaatttgt ggactcctac gatccaacca tagaaaacac ttttacaaag ttgatcacag
      301 taaatggaca agaatatcat cttcaacttg tagacacagc cgggcaagat gaatattcta
      361 tctttcctca gacatactcc atagatatta atggctatat tcttgtgtat tctgttacat
      421 caatcaaaag ttttgaagtg attaaagtta tccatggcaa attgttggat atggtgggga
      481 aagtacaaat acctattatg ttggttggga ataagaaaga cctgcatatg gaaagggtga
      541 tcagttatga agaagggaaa gctttggcag aatcttggaa tgcagctttt ttggaatctt
      601 ctgctaaaga aactcagann g
//
