LOCUS       sequence-257282-        7421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7421)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 7421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 51_pAAV-ProC12-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..7421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1532..1728
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1579..1714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      1735..1744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1741..2667
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(2671..2673,2674..2676,2677..3390)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2671..2673,2674..2676,2677..3387)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2716..2737)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2977..2996)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3324..3345
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3418..4006
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3471..3491)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3889..3900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4038..4514
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4237..4256)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4554..4694
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4554..4683
                     /label=AAV2 ITR
     rep_origin      4769..5224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4856..4875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5066..5087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5241..5260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5360..5382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5420..5438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5506..5610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5611..5679,5680..6471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5829..5848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6642..7230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7131..7150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   7292..7421
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gcggccgcac gcgttcaagc ctcctaccgc ctttgttatg caaacatatc aaacgccctc
       61 ctttgttatg caaaagggct ggaacggggc ctttgttatg caaatcgccc tccccgatcc
      121 ctttgttatg caaatttgac gaattcccac ctttgttatg caaacaaatc tcctaccctc
      181 ctttgttatg caaagtgaga ggggctgcac ctttgttatg caaaatgcgg cccctgagac
      241 ctttgttatg caaaaaccat gtacgcttgc ctttgttatg caaaccgcct gttgcttggc
      301 ctttgttatg caaagccacg cgattggcgc ctttgttatg caaagctcgg ttatgtacac
      361 ctttgttatg caaagctact ttaaacttgc ctttgttatg caaatcacga cctgaccgtc
      421 ctttgttatg caaaaacggt tgaaatagtc ctttgttatg caaaatgata ttgaatagtc
      481 ctttgttatg caaaaattta gatgccgacc ctttgttatg caaaggaatg ggcgtgctgc
      541 ctttgttatg caaattttcg ctgcgacagc ctttgttatg caaacatgct cgccactcac
      601 ctttgttatg caaaggtcta acaatgaccc ctttgttatg caaactacgt ggaatagatc
      661 ctttgttatg caaaccccga gtttttgaac ctttgttatg caaaatcagt aacttcattc
      721 ctttgttatg caaaatgtga cttaacctcc ctttgttatg caaatacacg ctagtcgcat
      781 ggacgtggat gctagctaaa acccagcagg ctcggttgtt gtagttctta cataatgact
      841 atcacctcgg caagggagtt ttaagcggca gcgggagaat tgccgcatga acacaaactt
      901 tctctgtcga tggttcgcgg aactcttcgg cgattgtccg cccctttcaa ccacctaaat
      961 agatgtgctt tttgggttga ggtgctgttc caaagaactg gcacgcatcg aagcacgtca
     1021 aaatcggctc gtagaaattc gcacgtattc cccgtgcctg gtgggtcgtc gagattctgt
     1081 gaaggagcac tgggaacgaa tgcgtgggtc accatacccg tagaatgctt gtaacgccac
     1141 tagcggcttg gtcatctttc cctaaaggct gcgtggtccc gaatgacgta acgtcgatgg
     1201 ccggttagcc caaaaatatg cccgtattta ctaaacctgt cgtgtctctc ttcctgttgt
     1261 ctctcgctag gtccatccct aagtcttatc gaaggtgcta ttttactctg ctcttggaac
     1321 acacccatct ccacgcacgt gataggtaca gcaagcgtta caggccatcg tttcgtacga
     1381 attttcgaaa agtaatcctc agaaatcgaa tattaccttt agttggaacg cgcttccaaa
     1441 gcgccgggcc ctgaagtgct atgggatggg cttctttcgc gtgtcctttt ggccgatgtc
     1501 tgactgacag attagctcga gatctgcgat ctgcatctca attagtcagc aaccatagtc
     1561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1621 catcgctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaagg atccgccacc
     1741 atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
     1801 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc gggctggatt
     1861 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg tgctgcaatg gcttgctgct
     1921 ggcttctcca tcctactgct tatgttttac gcctaccaaa catggaagtc aacctgcggc
     1981 tgggaggaga tctatgtgtg cgctatcgag atggtcaagg tgattctcga gttcttcttc
     2041 gagtttaaga acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt
     2101 tacgccgagt ggcttctcac ctgcccggtc atttgcattc acctgtcaaa cctgacgggc
     2161 ttgtccaacg actacagcag gcgcaccatg ggtctgcttg tgtctgatat tggcacaatt
     2221 gtgtggggcg ccacttccgc catggccacc ggatacgtca aggtcatctt cttctgcctg
     2281 ggtctgtgtt atggtgctaa cacgttcttt cacgctgcca aggcctacat cgagggttac
     2341 cacaccgtgc cgaagggccg gtgtcgccag gtggtgactg gcatggcttg gctcttcttc
     2401 gtatcatggg gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg
     2461 agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
     2521 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat ccacggcgac
     2581 attcgcaaga ccaccaaatt gaacattggt ggcactgaga ttgaggtcga gacgctggtg
     2641 gaggacgagg ccgaggctgg cgcggtaccc atggtgagca agggcgagga gctgttcacc
     2701 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2761 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2821 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     2881 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2941 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     3001 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     3061 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     3121 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     3181 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     3241 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     3301 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     3361 actctcggca tggacgagct gtacaagtga taagaattcg atatcaagct tatcgataat
     3421 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3481 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3541 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3601 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3661 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3721 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3781 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3841 tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3901 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3961 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgagcg
     4021 ctgctcgaga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     4081 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
     4141 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     4201 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     4261 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     4321 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     4381 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     4441 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     4501 ccttccctgt ccttctgatt ttgtaggtaa ccacgtgcgg accgagcggc cgcaggaacc
     4561 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4621 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     4681 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4741 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     4801 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     4861 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4921 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4981 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5041 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5101 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     5161 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     5221 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5281 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5341 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5401 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5461 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5521 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     5581 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5641 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5701 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5761 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5821 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     5881 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5941 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6001 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6061 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6121 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6181 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6241 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6301 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6361 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6421 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6481 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     6541 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6601 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6661 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6721 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6781 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6841 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6901 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6961 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7021 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7081 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7141 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7201 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7261 ctggcctttt gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc
     7321 actgaggccg cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
     7381 gcgcagagag ggagtggcca actccatcac taggggttcc t
//
