LOCUS       sequence-257297-        6472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: 8_pAAV-ProC4-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        713..909
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      760..895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      916..925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             922..1848
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1852..1854,1855..1857,1858..2571)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1852..1854,1855..1857,1858..2568)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1897..1918)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2158..2177)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2505..2526
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2599..3187
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2652..2672)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3070..3081)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3219..3695
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3418..3437)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3735..3875
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3735..3864
                     /label=AAV2 ITR
     rep_origin      3950..4405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4037..4056)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4247..4268
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4422..4441)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4541..4563
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4601..4619)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4687..4791
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4792..4860,4861..5652)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5010..5029)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5823..6411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6312..6331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgccgctgg tggaaaatat ttaattaaag gtccgcatta
      181 gattaattat aaccacctgt aaagttaatt atagagtcct ccgcctttaa ttaactggga
      241 acgagggttt aattacccct gcgttttacg ttaattatat tgacggctat gcttaattat
      301 ggcgcactgt gtgtttaatt aaccacctgc aatttcttaa ttagcgattt tgggattttt
      361 aattaaagat gggatttgcg ttaattaaaa ttcaagtatg gattaattat gaaagtctaa
      421 gggtttaatt atcgtacttt tctatattaa ttaagatgtg taaggtcgtt aattaatcgt
      481 tcgaattcac ttaattatca agggcaattt tattaattac cagtgaggtg ttacttaatt
      541 acgcgttaca ggacgtttaa ttactaaatc gacatcggtt aattattcat aagcaagatg
      601 ttaattatgc taaagcgcac ttttaattaa aaccctgcaa tagcttaatt atgctttact
      661 tcaggattaa ttagtggacc aatccttttt aattagctcg agatctgcga tctgcatctc
      721 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
      781 agttccgccc attctccgcc ccatcgctga ctaatttttt ttatttatgc agaggccgag
      841 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      901 ttttgcaaag gatccgccac catggattat ggaggcgccc tgagtgccgt tgggcgcgag
      961 ctgctatttg taacgaaccc agtagtcgtc aatggctctg tacttgtgcc tgaggaccag
     1021 tgttactgcg cgggctggat tgagtcgcgt ggcacaaacg gtgcccaaac ggcgtcgaac
     1081 gtgctgcaat ggcttgctgc tggcttctcc atcctactgc ttatgtttta cgcctaccaa
     1141 acatggaagt caacctgcgg ctgggaggag atctatgtgt gcgctatcga gatggtcaag
     1201 gtgattctcg agttcttctt cgagtttaag aacccgtcca tgctgtatct agccacaggc
     1261 caccgcgtcc agtggttgcg ttacgccgag tggcttctca cctgcccggt catttgcatt
     1321 cacctgtcaa acctgacggg cttgtccaac gactacagca ggcgcaccat gggtctgctt
     1381 gtgtctgata ttggcacaat tgtgtggggc gccacttccg ccatggccac cggatacgtc
     1441 aaggtcatct tcttctgcct gggtctgtgt tatggtgcta acacgttctt tcacgctgcc
     1501 aaggcctaca tcgagggtta ccacaccgtg ccgaagggcc ggtgtcgcca ggtggtgact
     1561 ggcatggctt ggctcttctt cgtatcatgg ggtatgttcc ccatcctgtt catcctcggc
     1621 cccgagggct tcggcgtcct gagcgtgtac ggctccaccg tcggccacac catcattgac
     1681 ctgatgtcga agaactgctg gggtctgctc ggccactacc tgcgcgtgct gatccacgag
     1741 catatcctca tccacggcga cattcgcaag accaccaaat tgaacattgg tggcactgag
     1801 attgaggtcg agacgctggt ggaggacgag gccgaggctg gcgcggtacc catggtgagc
     1861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     1921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     1981 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2041 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2101 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2281 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2341 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2401 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2461 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2521 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtg ataagaattc
     2581 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2641 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2701 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2761 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2821 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2881 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2941 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     3001 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     3061 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     3121 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     3181 tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct
     3241 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     3301 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     3361 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     3421 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     3481 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     3541 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     3601 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     3661 tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg
     3721 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     3781 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     3841 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
     3901 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
     3961 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4021 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4081 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4141 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     4201 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4261 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     4321 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4381 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     4441 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     4501 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     4561 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     4621 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4681 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4741 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4801 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4861 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4921 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4981 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     5041 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5101 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5161 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5221 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5281 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     5341 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5401 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5461 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5521 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5581 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5641 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5701 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
