LOCUS       sequence-257303-       10330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing ADH2 and ALD6 from Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 10330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pKdH_P-GAP-ScADH2+P-GAP*-ScALD6
FEATURES             Location/Qualifiers
     source          1..10330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..1034
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     promoter        1043..1519
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             3020..3037
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3134..3380
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3381..3857
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             4902..4919
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5016..5262
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(6087..6902)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6087..6902)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6810..6829
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6894..6913
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(7067..7086)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    7281..8029
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(8064..8086)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8172..8312
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8227..8246
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8327..8344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8498..9086)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8578..8597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9257..10048,10049..10117))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9880..9899
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10118..10222)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10290..10308
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctcatgttt gacagcttat catcgataag ctgactcatg ttggtattgt gaaatagacg
       61 cagatcggga acactgaaaa ataacagtta ttattcgaga tctaacatcc aaagacgaaa
      121 ggttgaatga aacctttttg ccatccgaca tccacaggtc cattctcaca cataagtgcc
      181 aaacgcaaca ggaggggata cactagcagc agaccgttgc aaacgcagga cctccactcc
      241 tcttctcctc aacacccact tttgccatcg aaaaaccagc ccagttattg ggcttgattg
      301 gagctcgctc attccaattc cttctattag gctactaaca ccatgacttt attagcctgt
      361 ctatcctggc ccccctggcg aggttcatgt ttgtttattt ccgaatgcaa caagctccgc
      421 attacacccg aacatcactc cagatgaggg ctttctgagt gtggggtcaa atagtttcat
      481 gttccccaaa tggcccaaaa ctgacagttt aaacgctgtc ttggaaccta atatgacaaa
      541 agcgtgatct catccaagat gaactaagtt tggttcgttg aaatgctaac ggccagttgg
      601 tcaaaaagaa acttccaaaa gtcggcatac cgtttgtctt gtttggtatt gattgacgaa
      661 tgctcaaaaa taatctcatt aatgcttagc gcagtctctc tatcgcttct gaaccccggt
      721 gcacctgtgc cgaaacgcaa atggggaaac acccgctttt tggatgatta tgcattgtct
      781 ccacattgta tgcttccaag attctggtgg gaatactgct gatagcctaa cgttcatgat
      841 caaaatttaa ctgttctaac ccctacttga cagcaatata taaacagaag gaagctgccc
      901 tgtcttaaac cttttttttt atcatcatta ttagcttact ttcataattg cgactggttc
      961 caattgacaa gcttttgatt ttaacgactt ttaacgacaa cttgagaaga tcaaaaaaca
     1021 actaattatt cgaagactag tctttttgta gaaatgtctt ggtgtcctcg tccaatcagg
     1081 tagccatctc tgaaatatct ggctccgttg caactccgaa cgacctgctg gcaacgtaaa
     1141 attctccggg gtaaaactta aatgtggagt aatggaacca gaaacgtctc ttcccttctc
     1201 tctccttcca ccgcccgtta ccgtccctag gaaattttac tctgctggag agcttcttct
     1261 acggccccct tgcagcaatg ctcttcccag cattacgttg cgggtaaaac ggaggtcgtg
     1321 tacccgacct agcagcccag ggatggaaaa gtcccggccg tcgctggcaa taatagcggg
     1381 cggacgcatg tcatgagatt attggaaacc accagaatcg aatataaaag gcgaacacct
     1441 ttcccaattt tggtttctcc tgacccaaag actttaaatt taatttattt gtccctattt
     1501 caatcaattg aacaactata tgactaagct acactttgac actgctgaac cagtcaagat
     1561 cacacttcca aatggtttga catacgagca accaaccggt ctattcatta acaacaagtt
     1621 tatgaaagct caagacggta agacctatcc cgtcgaagat ccttccactg aaaacaccgt
     1681 ttgtgaggtc tcttctgcca ccactgaaga tgttgaatat gctatcgaat gtgccgaccg
     1741 tgctttccac gacactgaat gggctaccca agacccaaga gaaagaggcc gtctactaag
     1801 taagttggct gacgaattgg aaagccaaat tgacttggtt tcttccattg aagctttgga
     1861 caatggtaaa actttggcct tagcccgtgg ggatgttacc attgcaatca actgtctaag
     1921 agatgctgct gcctatgccg acaaagtcaa cggtagaaca atcaacaccg gtgacggcta
     1981 catgaacttc accaccttag agccaatcgg tgtctgtggt caaattattc catggaactt
     2041 tccaataatg atgttggctt ggaagatcgc cccagcattg gccatgggta acgtctgtat
     2101 cttgaaaccc gctgctgtca cacctttaaa tgccctatac tttgcttctt tatgtaagaa
     2161 ggttggtatt ccagctggtg tcgtcaacat cgttccaggt cctggtagaa ctgttggtgc
     2221 tgctttgacc aacgacccaa gaatcagaaa gctggctttt accggttcta cagaagtcgg
     2281 taagagtgtt gctgtcgact cttctgaatc taacttgaag aaaatcactt tggaactagg
     2341 tggtaagtcc gcccatttgg tctttgacga tgctaacatt aagaagactt taccaaatct
     2401 agtaaacggt attttcaaga acgctggtca aatttgttcc tctggttcta gaatttacgt
     2461 tcaagaaggt atttacgacg aactattggc tgctttcaag gcttacttgg aaaccgaaat
     2521 caaagttggt aatccatttg acaaggctaa cttccaaggt gctatcacta accgtcaaca
     2581 attcgacaca attatgaact acatcgatat cggtaagaaa gaaggcgcca agatcttaac
     2641 tggtggcgaa aaagttggtg acaagggtta cttcatcaga ccaaccgttt tctacgatgt
     2701 taatgaagac atgagaattg ttaaggaaga aatttttgga ccagttgtca ctgtcgcaaa
     2761 gttcaagact ttagaagaag gtgtcgaaat ggctaacagc tctgaattcg gtctaggttc
     2821 tggtatcgaa acagaatctt tgagcacagg tttgaaggtg gccaagatgt tgaaggccgg
     2881 taccgtctgg atcaacacat acaacgattt tgactccaga gttccattcg gtggtgttaa
     2941 gcaatctggt tacggtagag aaatgggtga agaagtctac catgcataca ctgaagtaaa
     3001 agctgtcaga attaagttgc atcatcatca tcatcattaa cctagggcgg ccgcgaatta
     3061 attcgcctta gacatgactg ttcctcagtt caagttgggc acttacgaga agaccggtct
     3121 tgctagattc taatcaagag gatgtcagaa tgccatttgc ctgagagatg caggcttcat
     3181 ttttgatact tttttatttg taacctatat agtataggat tttttttgtc attttgtttc
     3241 ttctcgtacg agcttgctcc tgatcagcct atctcgcagc tgatgaatat cttgtggtag
     3301 gggtttggga aaatcattcg agtttgatgt ttttcttggt atttcccact cctcttcaga
     3361 gtacagaaga ttaagtgaga tttttgtaga aatgtcttgg tgtcctcgtc caatcaggta
     3421 gccatctctg aaatatctgg ctccgttgca actccgaacg acctgctggc aacgtaaaat
     3481 tctccggggt aaaacttaaa tgtggagtaa tggaaccaga aacgtctctt cccttctctc
     3541 tccttccacc gcccgttacc gtcgctagga aattttactc tgctggagag cttcttctac
     3601 ggcccccttg cagcaatgct cttcccagca ttacgttgcg ggtaaaacgg aggtcgtgta
     3661 cccgacctag cagcccaggg atggaaaagt cccggccgtc gctggcaata atagcgggcg
     3721 gacgcatgtc atgagattat tggaaaccac cagaatcgaa tataaaaggc gaacaccttt
     3781 cccaattttg gtttctcctg acccaaagac tttaaattta atttatttgt ccctatttca
     3841 atcaattgaa caactatatg tctattccag aaactcaaaa agccattatc ttctacgaat
     3901 ccaacggcaa gttggagcat aaggatatcc cagttccaaa gccaaagccc aacgaattgt
     3961 taatcaacgt caagtactct ggtgtctgcc acaccgattt gcacgcttgg catggtgact
     4021 ggccattgcc aactaagtta ccattagttg gtggtcacga aggtgccggt gtcgttgtcg
     4081 gcatgggtga aaacgttaag ggctggaaga tcggtgacta cgccggtatc aaatggttga
     4141 acggttcttg tatggcctgt gaatactgtg aattgggtaa cgaatccaac tgtcctcacg
     4201 ctgacttgtc tggttacacc cacgacggtt ctttccaaga atacgctacc gctgacgctg
     4261 ttcaagccgc tcacattcct caaggtactg acttggctga agtcgcgcca atcttgtgtg
     4321 ctggtatcac cgtatacaag gctttgaagt ctgccaactt gagagcaggc cactgggcgg
     4381 ccatttctgg tgctgctggt ggtctaggtt ctttggctgt tcaatatgct aaggcgatgg
     4441 gttacagagt cttaggtatt gatggtggtc caggaaagga agaattgttt acctcgctcg
     4501 gtggtgaagt attcatcgac ttcaccaaag agaaggacat tgttagcgca gtcgttaagg
     4561 ctaccaacgg cggtgcccac ggtatcatca atgtttccgt ttccgaagcc gctatcgaag
     4621 cttctaccag atactgtagg gcgaacggta ctgttgtctt ggttggtttg ccagccggtg
     4681 caaagtgctc ctctgatgtc ttcaaccacg ttgtcaagtc tatctccatt gtcggctctt
     4741 acgtggggaa cagagctgat accagagaag ccttagattt ctttgccaga ggtctagtca
     4801 agtctccaat aaaggtagtt ggcttatcca gtttaccaga aatttacgaa aagatggaga
     4861 agggccaaat tgctggtaga tacgttgttg acacttctaa acatcatcat catcatcatt
     4921 aacctagggc ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg
     4981 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt
     5041 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg
     5101 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca
     5161 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg
     5221 gtatttccca ctcctcttca gagtacagaa gattaagtga gacgttcgtt tgtgcaagct
     5281 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
     5341 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
     5401 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
     5461 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
     5521 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
     5581 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta
     5641 tcgaatctaa atgtaagtta aaatctctaa ataaacttga agtcggacag tgagtgtagt
     5701 cttgagaaat tctgaagccg tatttttatt atcagtgagt cagtcatcag gagatcctct
     5761 acgccggacg catcgtggcc gacctgcagg gggggggggg cgctgaggtc tgcctcgtga
     5821 agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc agaaagtgag
     5881 ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt tgaacttttg
     5941 ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct tcaactcagc
     6001 aaaagttcga tttattcaac aaagccgccg tcccgtcaag tcagcgtaat gctctgccag
     6061 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc
     6121 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     6181 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     6241 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     6301 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt
     6361 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     6421 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
     6481 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     6541 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     6601 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     6661 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     6721 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     6781 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     6841 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc
     6901 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
     6961 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc
     7021 cccctgcagg tcggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     7081 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     7141 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     7201 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     7261 gagtcgcata agggagagcg tcgagtatct atgattggaa gtatgggaat ggtgataccc
     7321 gcattcttca gtgtcttgag gtctcctatc agattatgcc caactaaagc aaccggagga
     7381 ggagatttca tggtaaattt ctctgacttt tggtcatcag tagactcgaa ctgtgagact
     7441 atctcggtta tgacagcaga aatgtccttc ttggagacag taaatgaagt cccaccaata
     7501 aagaaatcct tgttatcagg aacaaacttc ttgtttcgaa ctttttcggt gccttgaact
     7561 ataaaatgta gagtggatat gtcgggtagg aatggagcgg gcaaatgctt accttctgga
     7621 ccttcaagag gtatgtaggg tttgtagata ctgatgccaa cttcagtgac aacgttgcta
     7681 tttcgttcaa accattccga atccagagaa atcaaagttg tttgtctact attgatccaa
     7741 gccagtgcgg tcttgaaact gacaatagtg tgctcgtgtt ttgaggtcat ctttgtatga
     7801 ataaatctag tctttgatct aaataatctt gacgagccag acgataatac caatctaaac
     7861 tctttaaacg ttaaaggaca agtatgtctg cctgtattaa accccaaatc agctcgtagt
     7921 ctgatcctca tcaacttgag gggcactatc ttgttttaga gaaatttgcg gagatgcgat
     7981 atcgagaaaa aggtacgctg attttaaacg tgaaatttat ctcatgatct ctgcctcgcg
     8041 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8101 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8161 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     8221 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8281 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     8341 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8401 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8461 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8521 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8581 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8641 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8701 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8761 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8821 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8881 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     8941 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9001 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9061 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9121 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9181 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9241 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9301 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9361 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9421 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9481 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9541 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     9601 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9661 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9721 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9781 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9841 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     9901 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9961 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10021 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10081 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10141 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10201 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10261 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    10321 aagaattaat
//
