LOCUS       sequence-257313-        7220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree GR
  TITLE     Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF 
            complex to chromatin after T lymphocyte receptor signaling.
  JOURNAL   Cell. 1998 Nov 25. 95(5):625-36.
  PUBMED    9845365
REFERENCE   2  (bases 1 to 7220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1998 
            Nov 25. 95(5):625-36."
COMMENT     Sequence Label: pBS hBAF53b
FEATURES             Location/Qualifiers
     source          1..7220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1947..2402
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2034..2053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2244..2265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2529..2551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2543..2560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2544..2560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2567..2586
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2567..2585
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2610..2626
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2611..2627
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6922..6938)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6922..6938)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(6975..6993)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7014..7030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7014..7030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7027..7049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7038..7054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7062..7068,7069..7086,7087..7092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7107..7128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     1981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     2041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     2101 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     2161 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     2221 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     2281 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     2341 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     2401 ttgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2461 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2521 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     2581 atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata agcttgatat
     2641 cgaattcctt tcgttacttt tttgggtggc ggtaggaggt tatgacaaga gactgacagc
     2701 ttaaaaacta ctcaaatatt aggggtatgc cacaatcact cgggatctgg tcccggtggc
     2761 cacacgcatg cgggcagtgt gggtgctgag ctgctggagg agtagctctc cactccctga
     2821 cctgtgtccc cctttaccac ctcgtaggtt catggcctct ttcttcagag gcatggggtg
     2881 gggaggtatg gtgagcaggt tagggagggt cgatgttgaa tttttattgt tattactatg
     2941 ttcacagatt cttggagctg ataattattt caaatctaaa gaatgtgata ttaccacccc
     3001 ctttgtcaaa cttaagaaaa aaaaaacact atcactgaca aatagggacc atttctgaaa
     3061 atacacacac ctttggcgct ggccacaaac ccacagactg ttatcacagg gtctgcacta
     3121 actctggaaa tctagataat attctgtttt cttacagaat gacaatgtcc tcaatagcat
     3181 tatgtgtatc tgtgtgtttc ttgactgtat ggactccctg tacttgcaga taaagaagta
     3241 aatgtagtgc gcagcagggt ttgtgagttc cctattttct taacgaccat gaggtctgaa
     3301 gactgacaat tcattgtata cacacagacc agaatatttt catccgcatc atcacgcggc
     3361 tttagttgtc cactcacagg aggaaaaaca caattaaaga acaaggcttt tttttgtttt
     3421 gtttttgttt ttttttttgc agtaacacta agaagtatag ttaatgcata cacatgaagt
     3481 gtgacaaaac ttggcaaaaa cactgcagag gaaagtttga ttttttttaa ccagcgaaaa
     3541 atttatctca cttctaaaga tcttatagat acttaaaaac aaaaatctca aatctaaaac
     3601 aataaccgat aaagctttaa atgttgaatt ttaactctga ggatggggaa aaaatggatc
     3661 ttttctacat aaagactcaa ttgttcccta atcttagaga tggctattag agccaagtct
     3721 gctgaaaaca tcctgtttac tctgcagaga aaaacaggct tggcgtaagc attttgctca
     3781 gcatgcagac attttcattc tgttttagag aagagtcaag atggcagaat ataaaaaatg
     3841 aaattaaaaa gtacacagca tctgaggtca aaatggaatc tggctgttaa ccttacaaaa
     3901 atcaccccct cacccaaaaa aaaaagtggt aaaaatgagt gaatagttgc aaaaagttaa
     3961 cagcctatga gcttctagtt tctgcgagat atatgtgtta ataaaggtgc ttccaaattc
     4021 tcaggcagga gttttatctt ttcctttctt tttcctcttg cataccttct ataccatatt
     4081 caacccacag gagtttggaa gaaccgattg ctaacttatg ctacattcat gtcagaagag
     4141 gtgggaaaat tcttacaaca ggagccatac tcataggaaa tcaagttgct ataaataaaa
     4201 ctaactagca ataaatggat aagtgattta ttggggggac actgattaaa taatttggtt
     4261 tggaacctta acttgcaaca ctgctaagtt aactcctatg aatgttgtaa atgaaacacg
     4321 tccagggttg gggactgagg gatcagagat gttacatgag ccatccattt ctttacagag
     4381 tctcagagat ctgaactctt ttccacactt aaagttaggt catgatactg gatctaattg
     4441 acagcagagg tcctctgtct ggtgtgagac acccctttct ctattagtgc ttcttttctt
     4501 ttggctactc gtgtggtagg tagaaaccaa gtttgtgatg ttcaggagat gtacacacct
     4561 ctaagccact agtaatcaag tgttttaaaa agtagttaaa aaaaaaaaaa gggaaagctc
     4621 aaattaacat aatcaaaatg tttttaaaat aaatctatgt aatgagaggt gaccagcaag
     4681 cctgtatcta tgccagagtg tggtagaaga gaagaaggca ctctcactga attttataag
     4741 tatactgaag tcacctaacc tcttagtaga catggctctt gccaaagaat accacggaaa
     4801 gagctcttct cttccatgac atcctcacca tatcctgttc ttaggtcact gccaaccaat
     4861 aaagcaaagc tcccagcaag ttgacaaaaa gaagggggag gtctggggga aaaggggagg
     4921 agacgaagga caagagcaga acaacaggaa ccccttctat ttgcccaaga caaagagact
     4981 ttccaaatca ctgcagttgt cacaattaca cacctggagg aatcaatgca cagtaatgga
     5041 gggcacacga cacaggaagg aacttggcat tctacattac tgagacataa gcatattaac
     5101 ttgcttgacg tttgtttatt tattttaaat tcagcccact gcctttgggg cctgtccaga
     5161 accactccaa tttcctttat gggtgagtat cttacatctg tggagtgact ggaccacaca
     5221 cacacacatg tgcgtccccc ttgctcagga tgtctgggtg tatgtacgaa ggcactaaag
     5281 cggttttaga gaaattgcag gcagcaaccc cacccactcc ctttacctgt cgagctggag
     5341 tttccttccc actgggaagc ttaatgttta aaagaagctg tggtcatgct tgggaattct
     5401 gacccctggc cccaggaggg aatacaaaag aattcaacat caccaaaaac tgaaaggaaa
     5461 cactccacat ctccggctaa gggatgggga taattaagca gcagatcttc aaaacagcaa
     5521 ttaaaacgag cacaacagta gctgggagct aaaaaagtaa aatcacttgt tccttattag
     5581 attcttctat attttctcct cctcccaaaa caaacggagg taagacagac attcttcatt
     5641 aataggacag tccatagata attttcctct agtaatgcct atatttaaaa tttgttccaa
     5701 aaattattaa cctatatttc ctaactcttt acatagattc tcagcaatcc aactgtacta
     5761 ttttacagat tacttttttt tttaagctgt tgtttcttcc ttccttcctt tcttttgttg
     5821 ctggggttgt ggggacaagg agaagggttc ctttaaaaaa ataaaaatta ctaggggaga
     5881 tttcagttca gtctcaaatg gaaaggaaca aagtgatggc attctcatct atatacacca
     5941 aacactctcc cattaaacta cacacaattt caaaagaatc catgatcgag ctcttacaaa
     6001 gcttaatccg gaattccaac acgtcaagtc tgagccaaac ctgcacccag tgctcatgtc
     6061 cgaggctccg tggaacacac gggccaagcg ggagaagctg acagagctga tgttcgagca
     6121 gtacaacatt cctgccttct tcttatgcaa gacggctgtg ctcaccgcct ttgcaaacgg
     6181 gcggtccact ggcctcgtgc tggacagtgg agccacccac accacggcca ttccagtaca
     6241 tgacggctac gttctgcagc aaggcatcgt caagtcccct ctggcagggg acttcatctc
     6301 catgcagtgc cgggagctgt tccaggagat ggccattgac atcatcccac cttacatgat
     6361 cgcagccaag gagcctgtcc gggagggtgc ccccccaaac tggaagaaga aggagaagct
     6421 accccaggtc tccaagtcct ggcataacta catgtgtaat gaggtgatcc agaacttcca
     6481 ggcctccgtg ctgcaggtct cagactcccc ctacgatgaa caggtggctg cacaaatgcc
     6541 cacagtgcac tacgagatgc ccaatggcta caatacagac tacggcgccg agcgactccg
     6601 catccctgag ggcctgtttg atccctcgaa cgtcaagggc ctgtcgggga acaccatgtt
     6661 gggtgtgggc cacgtggtga ccaccagcat cggcatgtgt gacattgata ttcgcccggg
     6721 cctgtacggg agtgtcattg tcaccggcgg gaacacactg ctgcagggct tcactgacag
     6781 gctcaatcga gagctttccc agaagacccc accgagcatg cgactgaaac tcattgccag
     6841 caacagcacc atggagcgca agttcagccc ctggatcggg ggttccatcc tggcctcact
     6901 gggaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc
     6961 tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     7021 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7081 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7141 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7201 cgcgcgggga gaggcggttt
//
