LOCUS       sequence-257316-        4864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing CocE enzyme (cocaine to benzoate) gene under control of 
            the constitutive promoter J23101, and RBS B0032.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pSB4C5_J23101-CocE
FEATURES             Location/Qualifiers
     source          1..4864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      531..588
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      655..749
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1241..1463
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1511..2461
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELTTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             2777..3436
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2843..2862)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        3576..3610
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
ORIGIN
        1 cacaacggtg acaacggtcc ggctgaccag cgtccgatcc acgaccgtga cgacgttctg
       61 tgctactcta ccgaagttct gaccgacccg gttgaagtta ccggtaccgt ttctgctcgt
      121 ctgttcgttt cttcttctgc tgttgacacc gacttcaccg ctaaactggt tgacgttttc
      181 ccggacggtc gtgctatcgc tctgtgcgac ggtatcgttc gtatgcgtta ccgtgaaacc
      241 ctggttaacc cgaccctgat cgaagctggt gaaatctacg aagttgctat cgacatgctg
      301 gctacctcta acgttttcct gccgggtcac cgtatcatgg ttcaggtttc ttcttctaac
      361 ttcccgaaat acgaccgtaa ctctaacacc ggtggtgtta tcgctcgtga acagctggaa
      421 gaaatgtgca ccgctgttaa ccgtatccac cgtggtccgg aacacccgag ccacatcgtt
      481 ctgccgatca tcaaacgtta agcttcggtg cttaatgcgt cttcctgcag tccggcaaaa
      541 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
      601 atgcaggtac gactttctgc tagagctgtc agaccaagtt tacgagctcg cttggactcc
      661 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      721 gccgccgggc gttttttatt ggtgagaatc caagcactag ggacagtaag acgggtaagc
      781 ctgttgatga taccgctgcc ttactgggtg cattagccag tctgaatgac ctgtcacggg
      841 ataatccgaa gtggtcagac tggaaaatca gagggcagga actgctgaac agcaaaaagt
      901 cagatagcac cacatagcag acccgccata aaacgccctg agaagcccgt gacgggcttt
      961 tcttgtatta tgggtagttt ccttgcatga atccataaaa ggcgcctgta gtgccattta
     1021 cccccattca ctgccagagc cgtgagcgca gcgaactgaa tgtcacgaaa aagacagcga
     1081 ctcaggtgcc tgatggtcgg agacaaaagg aatattcagc gatttgcccg agcttgcgag
     1141 ggtgctactt aagcctttag ggttttaagg tctgttttgt agaggagcaa acagcgtttg
     1201 cgacatcctt ttgtaatact gcggaactga ctaaagtagt gagttataca cagggctggg
     1261 atctattctt tttatctttt tttattcttt ctttattcta taaattataa ccacttgaat
     1321 ataaacaaaa aaaacacaca aaggtctagc ggaatttaca gagggtctag cagaatttac
     1381 aagttttcca gcaaaggtct agcagaattt acagataccc acaactcaaa ggaaaaggac
     1441 atgtaattat cattgactag cccatctcaa ttggtatagt gattaaaatc acctagacca
     1501 attgagatgt atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta
     1561 tgacttaacg gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac
     1621 gattgaaaac cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca
     1681 gatgatgaac atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct
     1741 gacgacgaga actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg
     1801 gacaaactat gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt
     1861 gccttatctt ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt
     1921 tgaaaacaaa tactccatga ggatttatga gtggttatta aaagaactaa cacaaaagaa
     1981 aactcacaag gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga
     2041 aaataactac catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga
     2101 tttaaacact tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac
     2161 gttgattttc caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa
     2221 ccagataaaa atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca
     2281 taacggacta agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt
     2341 tgaggcaaaa tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg
     2401 gctcacgcaa aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg
     2461 aggttcttat ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa
     2521 caactgttca ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg
     2581 taaaaaagat agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc
     2641 atgaacagat cgacaatgta actactagag gttgatcggg cacgtaagag gttccaactt
     2701 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     2761 agctaaggaa gctaaaatgg agaaaaaaat cacgggatat accaccgttg atatatccca
     2821 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2881 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2941 ttatccggcc tttattcaca ttcttgcccg cctgatgaac gctcacccgg agtttcgtat
     3001 ggccatgaaa gacggtgagc tggtgatctg ggatagtgtt cacccttgtt acaccgtttt
     3061 ccatgagcaa actgaaacgt tttcgtccct ctggagtgaa taccacgacg atttccggca
     3121 gtttctccac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     3181 taaagggttt attgagaata tgttttttgt ctcagccaat ccctgggtga gtttcaccag
     3241 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca cgatgggcaa
     3301 atattatacg caaggcgaca aggtgctgat gccgctggcg atccaggttc atcatgccgt
     3361 ttgtgatggc ttccatgtcg gccgcatgct taatgaatta caacagtact gtgatgagtg
     3421 gcagggcggg gcgtaataat actagctccg gcaaaaaaac gggcaaggtg tcaccaccct
     3481 gccctttttc tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg tctaagacga
     3541 tactgttcga ggatactaga ggatgacccc atctgtttac agctagctca gtcctaggta
     3601 ttatgctagc tagtagagtc acacaggaaa gtagtagatg atggttgacg gtaactactc
     3661 tgttgcttct aacgttatgg ttccgatgcg tgacggtgtt cgtctggctg ttgacctgta
     3721 ccgtccggac gctgacggtc cggttccggt tctgctggtt cgtaacccgt acgacaaatt
     3781 cgacgttttc gcttggtcta cccagtctac caactggctg gaatttgttc gtgacggtta
     3841 cgctgttgtt atccaggaca cccgtggtct gttcgcttct gaaggtgaat ttgttccgca
     3901 cgttgacgac gaagctgacg ctgaagacac cctgtcttgg attttggaac aggcttggtg
     3961 cgacggtaac gttggtatgt tcggtgtttc ttacctgggt gttacccagt ggcaggctgc
     4021 tgtttctggt gttggtggtc tgaaagctat cgctccgtct atggcttctg ctgacctgta
     4081 ccgtgctccg tggtacggtc cgggtggtgc tctgtctgtt gaagcgctgc tgggttggtc
     4141 tgctctgatc ggtaccggtc tgatcacctc tcgttctgac gctcgtccgg aagacgctgc
     4201 tgacttcgtt cagctggctg ctatcctgaa cgacgttgct ggtgctgctt ctgttacccc
     4261 gctggctgaa cagccgctgc tgggtcgtct gatcccgtgg gttatcgacc aggttgttga
     4321 ccacccggac aacgacgaat cttggcagtc tatctctctg ttcgaacgtc tgggtggtct
     4381 ggctaccccg gctctgatca ccgctggttg gtacgacggt ttcgttggtg aaagcctgcg
     4441 taccttcgtt gctgttaaag acaacgctga cgctcgtctg gttgttggtc cgtggtccca
     4501 ctctaacctg accggtcgta acgctgaccg taaattcggt atcgctgcta cctacccgat
     4561 ccaggaagct accaccatgc acaaagcttt cttcgaccgt cacctgcgtg gtgaaaccga
     4621 cgcacttgct ggtgttccga aagttcgtct gttcgttatg ggtatcgacg aatggcgtga
     4681 cgaaaccgac tggccgctgc cggacaccgc ttacaccccg ttctacctgg gtggttctgg
     4741 tgctgctaac acctctaccg gtggtggtac cctgtctacc tctatcagcg gtaccgaatc
     4801 tgctgacacc tacctgtacg acccggctga cccggttccg agcctgggtg gtaccctgct
     4861 gttc
//
