LOCUS       sequence-257322-        7425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 587, 
            Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7425)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..7425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        116..419
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        420..623
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     574..594
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      662..671
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             668..1375
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(813..831)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1081..1100
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    3194..3205
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4268..4389
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4305..4324)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4359..4378
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4396..4851)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4533..4554)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4745..4764
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4878..4982
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4984..5341
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4984..5175
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5007..5027)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5192..5327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5376..6170
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5376..6170
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5430..5449)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6040..6059
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6358..6377)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6402..6449
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6778..7366
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7267..7286
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt
       61 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      421 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      481 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      541 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      601 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt
      661 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
      721 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      781 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
      841 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
      901 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
      961 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     1021 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     1081 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     1141 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     1201 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     1261 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     1321 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg
     1381 actcagatct ggcagcggtg gaggcttgga ggagccggag gccgcgacgc ggacagccgc
     1441 ggccgtggat tgcaaagaca gacccgggtt cccagtgaag aggttaatac aagcccgctt
     1501 gcccttcaaa cgcctgaacc ttgttcccaa ggagaaagtt gaggaagaca catcaccaaa
     1561 agcagctgtg gaaagcaaag tcccggatct acaattgtct ttgggcactt ttgaaagcca
     1621 gtgtcacaca ggctctcacg taggcttgag tacaaagttg gtcggtggcc agggccccat
     1681 tgatagcttc ttgagggcaa caatcaagcc agtaccgagt gtggtcatta tcgacctgac
     1741 ggagaactgc agtgacattc cagacagccc tgagggccac agtgaactaa gtcctgacac
     1801 tgctggtgtg gtcaccacag tggaaggagc tgccaagcag caggagcact ctgcagcaga
     1861 gctgtgtctc ctagagaccc cttcagacat cacttgtcac atggaagagg agcctgggag
     1921 tccaggagac ccaaagagga cgggtgactg tcaggctggt tcactacaga gctgtccgga
     1981 gctgacccca ggttctagga catgccccac caaggagtta tcaagctgga gcaaggcagg
     2041 ggatctccta tttatagaga aagtgcctgt ggtggtgcta gaggacatcc tggccactaa
     2101 gccctccatt gcctctcttc ccatgatgtc cctggatagg agtgtcactt cagagagtga
     2161 aatactggag tcctgccccg aggatgactc catactgagc cactcctcca ccaactcctc
     2221 ttctccaacc agctctcctg agggaccatc cacaccccca gaacatcggg gtgggagaag
     2281 ctccccctcc accccagcct gcagagttgc caagaacttt gtcaaaggct ccacagagaa
     2341 gggcaggagc aaactgcaca gagatagaga acagcagagg gaagagaagg aaaagctgag
     2401 ggaagagata cgacgagcca aggaggaggc acggaagaag aaggaggagg agaaggagct
     2461 gaaggaaaag gagcggaggg aaaagcggga gaaagatgag aaggagaagg cggagaagca
     2521 gcggctaaag gaggaaaaac gcaaggagcg gcaggaagct ctggaggcta aactggagga
     2581 gaagaggaag aaggaggaag agaagcggtt acgggaggaa gagaagcggt tacgggaaga
     2641 agagaagcgc attaaggcag aaaaggcaga gatcactcga ttcttccaga aaccgaagac
     2701 tccgcaggct cccaagaccc tggccggttc ctgtggtaag tttgccccct ttgaaatcaa
     2761 agaacacatg gtgctggctc cacggtgccg agctgccttg gatcaagacc tgtgcgatca
     2821 gctggaccag ctcctgcagc agcagagtgt ggcaagcacc ttcctcagtg acctaaaaag
     2881 ccggctgccc ttgagatcgg ggcccacccg ggtctgtggc catgacacag acatcatgaa
     2941 cagggatgtg gtcattgtgg agagcagcaa ggtggacggt gtgtctgaaa ggaagaagtt
     3001 tggccgcatg aagcttctgc agttctcaga gaaccaccgg ccagcatact gggggacgtg
     3061 gaacaagaag acagccatca tccgcccccg gaacccctgg gcccaggaca aagaccttct
     3121 tgactatgag gtagacagtg acgatgagtg ggaagaggag gaacctgggg agtctttgtc
     3181 ccacagtgag ggggatgagg atgacgatgt gggggaagat gaggatgagg acgatggctt
     3241 ctttgtgccc catgggtact tatctgaaga tgaaggcgtg actgaggagt gtgcagaccc
     3301 tgagaaccac aaagtacacc agaagctgaa ggccaaggag tgggatgagc tcctagccaa
     3361 gggcaagcgc tttcgtgtgc tgcagcctgt gcacgtggga tgtgtatggg catcagaggc
     3421 agccaactgc acgagttccg acttgaagct gctgcagcag ttcactgcat gcctgctgga
     3481 tgtggcatcc cctgatgagc cagaacccgg ggcctccagg agggaaaaga gggaccagca
     3541 tatcctggcc cagttgcttc cgctgctaca tggcaatgtg aatgggagca aggtgatcat
     3601 ccatgaattc caggagcaat gccgcagggg cctgctgacc cttccaagtc ccaccccaca
     3661 cctgcagatg cccaacttgg aggatgctgt tgctgtgcca tccaaggcca ggctgaagcg
     3721 ccttatctct gagaactctg cctatgagaa gcggcccaac ttccgtatgt gctggtatgt
     3781 gcacccagag gtgctcaaga gctttggcca ggagtgcctg ccagtgccct gccagtggac
     3841 ctacatcacc accatgccct cagcacccag ggaagacagt ggcagtgctt ctactgaggg
     3901 gcctggccag agcacaccta tgctactcaa gcggaagcca gcggccacca tgtgcatcac
     3961 gcagtttatg aagaagcggc gctatgatgg acaggttggg tctggggaca tggatggctt
     4021 ccaggcagac acagaagagg atgaagagga tgacacagac tgtatgatca tagatgtgcc
     4081 agatgttggg agtgacgtgt cagaagctcc catacctgct cccacacttt gcaagtgagg
     4141 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     4201 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     4261 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4321 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4381 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     4441 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     4501 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     4561 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     4621 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     4681 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     4741 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     4801 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     4861 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4921 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4981 gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5041 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5101 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5161 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5221 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5281 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     5341 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5401 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5461 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5521 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     5581 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5641 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5701 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5761 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5821 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5881 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     5941 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6001 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6061 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6121 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6181 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6241 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6301 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     6361 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     6421 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     6481 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     6541 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     6601 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     6661 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6721 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6781 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6841 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6901 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6961 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7021 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7081 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7141 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7201 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7261 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7321 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7381 ggccttttta cggttcctgg ccttttgctg gccttttgct cacat
//
