LOCUS       sequence-257323-        7149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myr mCherry DrTrkA expression under CAMK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pAAV-CW3SL-Myr-mCherry-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..7149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             578..1279
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(717..735)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     985..1004
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4032..4052)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   4412..4552
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4412..4541
                     /label=AAV2 ITR
     rep_origin      4627..5082
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4714..4733)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4924..4945
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5099..5118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5218..5240
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5278..5296)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5364..5468
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5469..5537,5538..6329)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5687..5706)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6500..7088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6989..7008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcag atgctgcaca cttgtggact aagtttgttc gcatcccctt
      181 ctccaacccc ctcagtacat caccctgggg gaacagggtc cacttgctcc tgggcccaca
      241 cagtcctgca gtattgtgta tataaggcca gggcaaagag gagcaggttt taaagtgaaa
      301 ggcaggcagg tgttggggag gcagttaccg gggcaacggg aacagggcgt ttcggaggtg
      361 gttgccatgg ggacctggat gctgacgaag gctcgcgagg ctgtgagcag ccacagtgcc
      421 ctgctcagaa gccccaagct cgtcagtcaa gccggttctc cgtttgcact caggagcacg
      481 ggcaggcgag tggcccctag ttctgggggc agcgtgaaaa gacggtatcg ataagcttgg
      541 taccatggga tgtattaaat caaaaaggaa agacgctagc aagggcgagg aggataacat
      601 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
      661 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      721 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
      781 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
      841 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      901 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
      961 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1021 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1081 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1141 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1201 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1261 catggacgag ctgtacaagg aattcagtgc tggtagtgct ggtagtgctg gcaccggtat
     1321 gagccgggac ccgttgccct tttttccacc gctttacctt ggtggcccgg aaattaccac
     1381 cgagaactgc gagcgcgagc cgattcatat tcccggcagc atccagccgc acggcgccct
     1441 gctcactgcc gacgggcaca gcggcgaggt gctccagatg agcctcaacg cggccacttt
     1501 tctgggacag gaacccacag tgctgcgcgg acagaccctc gccgcactgc tgcccgagca
     1561 gtggcccgcg ctgcaagcgg ccctgccccc cggctgcccc gacgccctgc aataccgcgc
     1621 aacgctggac tggcctgccg ccgggcacct ttcgctgacg gtgcaccggg tcggcgagtt
     1681 gctgattctg gagttcgagc cgacggaggc ctgggacagc accgggccgc acgcgctgcg
     1741 caacgcgatg ttcgcgctcg aaagtgcccc caacctgcgg gcgctggccg aggtggcgac
     1801 ccagacggtc cgcgagctga cgggctttga ccgggtgatg ctctacaaat ttgcccccga
     1861 cgccaccggc gaagtgattg ccgaggcccg ccgtgagggg ctgcacgcct ttctgggcca
     1921 ccgttttccc gcgtcggaca ttccggcgca ggcccgcgcg ctctacaccc ggcacctgct
     1981 gcgcctgacc gccgacaccc gcgccgccgc cgtgccgctc gatcccgtcc tcaacccgca
     2041 gacgaatgcg cccaccccgc tgggcggcgc cgtgctgcgc gccacctcgc ccatgcacat
     2101 gcagtacctg cggaacatgg gcgtcgggtc gagcctgtcg gtgtcggtgg tggtcggcgg
     2161 ccagctctgg ggcctgatcg cctgccacca ccagacgccc tacgtgttgc cgcccgacct
     2221 gcgaaccacg ctcgaatacc tgggccgctt gctgagcctg caagttcagg tcaaggaagc
     2281 ggcggacgtg gcggcctttc gccagagcct gcgggagcac cacgcgcggg tggccctcgc
     2341 ggcggcgcac tcgctctcgc cgcacgacac cctcagtgac ccggcgcttg acctgctggg
     2401 cctgatgcgg gccgggggcc tgattctgcg tttcgagggc cgctggcaga cgttgggtga
     2461 agtgccgcct gccccggcgg tggacgcgct gctggcgtgg ctcgaaaccc agccgggcgc
     2521 cctggtccag accgacgcgc taggccaact gtggcccgcc ggcgccgatc tcgcccccag
     2581 cgcagcgggc ctgctcgcca tcagcgtggg cgagggctgg tcggagtgcc tcgtctggct
     2641 gcggcccgaa ctgcggctgg aggtcgcctg gggcggggcc actcctgacc aggcgaaaga
     2701 cgacctcggg ccgcgccact cattcgacac ctacctcgaa gaaaaacgcg gctacgccga
     2761 gccctggcat cccggcgaaa tcgaggaggc gcaggatcta cgtgacacat tgaccggggc
     2821 gctgggcgag gcagaggcag cagcaaagga ggcagcagca aaggaagcgg cggcgaaaga
     2881 agcggcggcg aaagcgaaca aatgtggaca gaggagcaaa tttgggatca accgccctgc
     2941 tgtgctggcg ccagaggatg ggctggccat gtccctacac ttcatgacac tgggtggcag
     3001 ttctctttcc cctactgagg gcaaaggctc cggactccag ggccacatca tggagaaccc
     3061 acagtacttc agtgatacct gtgtccacca tatcaagcgc caggacatca ttctcaagtg
     3121 ggagctaggg gagggagcct ttggaaaggt ctttcttgct gagtgctaca accttctgaa
     3181 tgatcaggac aagatgctgg tggctgtcaa ggcactgaag gagacatctg agaatgctcg
     3241 tcaggacttc catcgtgagg cagagctgct caccatgcta cagcaccaac acatcgtacg
     3301 cttctttgga gtctgcacgg agggtggccc attgctcatg gtcttcgagt acatgcgcca
     3361 tggggacctc aaccgtttcc tccggtccca cggacctgat gcaaaactgc tggctggcgg
     3421 cgaggatgtg gctcctggtc ctttgggcct tgggcagctt ctggctgtgg ctagccaggt
     3481 ggctgctggt atggtgtacc tagccagcct gcactttgtg caccgggacc tggccacacg
     3541 caactgtctg gtgggtcagg gactagtggt caagattgga gacttcggca tgagcaggga
     3601 catctacagc acagactact accgtgtggg aggtcggacc atgctgccca tccgctggat
     3661 gcctccagag agcatcctct accgcaagtt cagcaccgag agtgatgtgt ggagcttcgg
     3721 ggtggtgctc tgggagatct tcacctatgg aaagcaaccc tggtaccagc tctccaacac
     3781 tgaggcgatc gagtgcatca cgcagggccg ggagctggag cggccgcgcg cctgccctcc
     3841 tgatgtctac gccatcatgc gcggctgctg gcagcgtgag ccgcaacagc gcctcagcat
     3901 gaaggatgtg cacgcgcggc tgcaggcctt ggcacaggcg ccaccgagtt acctggacgt
     3961 tctgggctag ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4021 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4081 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt
     4141 cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4201 ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt
     4261 gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     4321 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     4381 ggagatgtgg gaggtttttt aaagcggccg caggaacccc tagtgatgga gttggccact
     4441 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4501 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4561 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4621 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4681 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4741 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4801 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4861 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4921 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     4981 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     5041 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5101 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5161 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5221 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5281 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5341 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5401 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5461 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5521 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5581 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5641 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5701 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5761 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5821 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5881 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5941 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6001 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6061 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6121 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6181 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6241 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6301 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6361 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6421 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6481 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6541 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6601 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6661 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6721 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6781 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6841 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6901 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6961 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7021 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7081 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7141 ctcacatgt
//
