LOCUS       sequence-257332-        3185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtzA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7034
FEATURES             Location/Qualifiers
     source          1..3185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2186..2204)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2200..2247
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2265..3080)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2988..3007
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3072..3091
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttac tagagtcaag aggaaaatgc aaacgctttc
      721 cattcagcat ggtaccttgg tcacaatgga ccagtatcgc cgtgtcttag gcgattcgtg
      781 ggtgcacgtg caagatggcc gtatcgttgc tttgggggtc catgcagaat cggttcctcc
      841 acccgccgat cgcgtgatcg acgcgcgcgg gaaagtcgtg ctgccaggat ttattaatgc
      901 gcatacccat gtcaaccaga ttcttcttcg cggcggaccg agtcacgggc gccaatttta
      961 cgactggtta tttaatgtag tctacccagg gcagaaggcc atgcgtcccg aggacgtagc
     1021 tgtggcagtg cgcttgtact gtgcagaagc tgttcgttct ggcatcacta ctatcaatga
     1081 aaatgctgat agcgctattt atccaggcaa tatcgaggct gcaatggctg tgtatggaga
     1141 agttggggtt cgtgtcgtct atgcgcgtat gttttttgac cgcatggatg gtcgcattca
     1201 aggttacgtt gatgcgttga aggcgcgtag tccacaagtg gagctgtgtt caattatgga
     1261 agaaacagct gttgcaaagg accgcatcac ggcactttcc gatcaatatc atgggactgc
     1321 ggggggccgt atttcggtct ggcctgcccc ggctaccact acagccgtta ccgtggaggg
     1381 tatgcgctgg gcgcaggcat tcgctcgtga ccgcgccgtc atgtggaccc tgcacatggc
     1441 ggagtcagac cacgatgagc gcattcacgg tatgtcgcct gccgaataca tggagtgcta
     1501 cggccttctt gacgagcgct tacaggtggc ccattgcgtg tactttgatc gcaaggacgt
     1561 tcgcttactg catcgccata acgttaaagt ggcctctcag gtcgtgagca atgcgtatct
     1621 gggctccggc gttgcgccgg ttccagagat ggtggaacgt gggatggcag tcggaattgg
     1681 taccgacaac ggcaacagta atgattcagt taatatgatt ggagatatga aattcatggc
     1741 ccacatccat cgtgctgttc accgtgatgc cgatgtgctt acacccgaaa agatcttaga
     1801 gatggctacg atcgacggcg cacgcagtct tggtatggac catgaaatcg gatcaattga
     1861 gactggcaaa cgtgctgact taatccttct tgacttacgc catccgcaga cgactcccca
     1921 ccaccacctg gcagcgacta tcgtcttcca ggcatatgga aatgaggtag acacggtact
     1981 tatcgatggc aatgtggtga tggaaaaccg ccgccttagt ttccttcccc cggagcgtga
     2041 attagctttc cttgaagaag cacaaagtcg cgccacagct atcctgcaac gcgcaaacat
     2101 ggttgccaac cctgcatggc gtagccttta ataagtcgac cggctgctaa caaagcccga
     2161 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     2221 tctaaacggg tcttgagggg ttttttgctg aaagccaatt ctgattagaa aaactcatcg
     2281 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     2341 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     2401 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     2461 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     2521 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     2581 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     2641 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     2701 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     2761 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2821 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2881 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     2941 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     3001 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcttcga gcaagacgtt
     3061 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     3121 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     3181 acgtg
//
