LOCUS       sequence-257333-        3209 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AtzB expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7035
FEATURES             Location/Qualifiers
     source          1..3209
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             683..705
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(2210..2228)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2224..2271
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(2289..3104)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3012..3031
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3096..3115
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgactac
      721 taccctgtat acgggattcc atcaattagt gacaggtgac gtagcgggga ccgttctgaa
      781 tggtgtcgat attctggtac gtgatgggga aattatcggc ttgggcccag atttgcctcg
      841 cacactggca cccatcggcg ttgggcaaga gcaaggcgtc gaagtggtta attgtcgcgg
      901 acttaccgcg tatccgggtt taatcaatac gcaccatcac ttcttccagg cgtttgtccg
      961 caatcttgca ccccttgatt ggacccagtt ggacgtctta gcatggcttc gtaagatcta
     1021 tcccgttttt gccttagttg atgaggattg tatttatcat tcgaccgtag tctcgatggc
     1081 tgagctgatt aaacacggct gtacgactgc gtttgatcac caatataact actcgcgtcg
     1141 cgggggcccc ttcctggttg accgccagtt cgatgcagcc aatttgttgg gccttcgttt
     1201 tcatgcaggt cgcggatgca tcacgcttcc gatggccgag gggagcacta ttcccgatgc
     1261 gatgcgtgag agcacagaca cattccttgc agattgtgaa cgcctggttt ctcgtttcca
     1321 tgacccgcgc cctttcgcta tgcaacgtgt agttgttgct ccatcctctc cagtgattgc
     1381 ttatcccgaa acctttgtgg agtcagcacg tcttgcccgt catttaggtg ttagtttaca
     1441 cactcatctg ggggaaggag agacacctgc gatggtcgcc cgctttgggg agcgctcatt
     1501 agattggtgt gagaaccgtg gtttcgttgg tccagatgta tggttagcac acggatggga
     1561 atttaccgcg gccgatattg cgcgtcttgc cgctaccggc accggggtag cccattgtcc
     1621 tgccccggtc ttcttggttg gagctgaggt tacggatatt ccggcgatgg cagcagctgg
     1681 ggtacgtgta gggttcggtg tggacgggca cgcctcaaat gactcctcga acctggcaga
     1741 gtgtattcgc ttggcgtacc ttctgcagtg tctgaaggcc agcgagcgtc agcaccctgt
     1801 tcctgcgccg tacgatttct tacgtatggc tactcaaggg ggggcggact gcttaaatcg
     1861 tcctgactta ggcgccttag ctgtcgggcg cgcggctgac ttttttgccg tggatttaaa
     1921 ccgcattgag tatattggag cgaatcacga tcctcgttcc cttcccgcca aggtgggatt
     1981 ctccggaccc gtagacatga cagttattaa tggtaaggta gtctggcgca atggcgagtt
     2041 ccctggtctt gacgagatgg agcttgcacg cgctgcggac ggagtgttcc gccgcgttat
     2101 ttacggagat ccgttggttg ctgccttgcg tcgcggtacc ggggttactc cctgttaagt
     2161 cgaccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     2221 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagcc
     2281 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     2341 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     2401 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     2461 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     2521 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     2581 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     2641 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     2701 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     2761 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     2821 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     2881 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     2941 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     3001 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     3061 aatcgcggct tcgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     3121 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     3181 taacatcaga gattttgaga cacaacgtg
//
