LOCUS       sequence-257336-        2732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtzR expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Silverman AD, Akova U, Alam KK, Jewett MC, Lucks JB
  TITLE     Design and Optimization of a Cell-Free Atrazine Biosensor.
  JOURNAL   ACS Synth Biol. 2020 Mar 20;9(3):671-677. doi: 
            10.1021/acssynbio.9b00388. Epub 2020 Mar 3.
  PUBMED    32078765
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00388"; journalName: "ACS Synth Biol"; date: 
            "2020-03-20- 20"; volume: "9"; issue: "3"; pages: "671-677"
COMMENT     Sequence Label: pJBL7032
FEATURES             Location/Qualifiers
     source          1..2732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             683..705
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1733..1751)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1747..1794
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1812..2627)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2535..2554
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2619..2638
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac atatgcgcgc
      721 tcaacgtcct agtgcgattc aggcaattag cattccttcc tttctgttac gtcctccgat
      781 gcaacacctg cgctttttgc actacatcga cgctgtcgct cgctgcggat ccatccgtgc
      841 tgccgccgag cagcttcatg ttgcagcatc cgcggtcaac cgtcgcgttc aagatttaga
      901 atatgaactg ggtaccccaa tcttcgaacg tttaccccgc ggcgttcgcc tgaccgccgc
      961 gggtgaactg ttcgtcgcgt atgcgcgtcg tcgtaatgcc gatttagagc aggtgcaatc
     1021 gcaaatccaa gatttgagcg gcatgaaacg cggccgtgtc acgctggccg cctcacaagc
     1081 tttagccccg gaattcttac ctcgcgtaat tcacgcgttt caggcacaac gcccgggcat
     1141 cgcgttcgac gtaaaggtgc tggatcgcga gcgtgcggtt cttgcggtga ctgattttgc
     1201 ggcagattta gctctggtgt ttaatccccc tgatttacgc ggtcttacgg ttattgcaca
     1261 agcccgccaa cgtatctgtg cagttgtggc ctcggaccac ccccttgcta aacgtacttc
     1321 acttcgcctg aaggactgct tggactaccc attggccctt ccagactcgt cattgagcgg
     1381 ccgcaatgtc ttagatgaat tgtttgataa atcctcagca cgcccacgtc cgcaactggt
     1441 cagcaattca tatgagatga tgcgcggctt cgcccgtgag acgggggggg tatctttcca
     1501 aatcgaaatc ggggctggtt ctacggaggg ggaagtcgcc attccgatcg acgaacgttc
     1561 gctggcctcg gggcgtgtag ttctggttgc actgcgcgaa cgcgtacttc ctgtcgcatc
     1621 ggccgcattt gccgagttcg tcgcaggtaa attagcagac acgactcata acgcaaccta
     1681 agtcgaccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     1741 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
     1801 gccaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1861 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1921 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1981 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     2041 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     2101 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     2161 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     2221 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     2281 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     2341 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     2401 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     2461 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     2521 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2581 tttaatcgcg gcttcgagca agacgtttcc cgttgaatat ggctcataac accccttgta
     2641 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
     2701 atgtaacatc agagattttg agacacaacg tg
//
