LOCUS       sequence-257357-        9048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of FPR1-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Gripentrog JM, Kantele KP, Jesaitis AJ, Miettinen HM
  TITLE     Experimental evidence for lack of homodimerization of the G 
            protein-coupled human N-formyl peptide receptor.
  JOURNAL   J Immunol. 2003 Sep 15;171(6):3187-93.
  PUBMED    12960347
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Immunol."; date: "2003-09-15"; volume: "171(6)"; pages: "3187-93"
COMMENT     Sequence Label: pBGSA FPR1-EGFP
FEATURES             Location/Qualifiers
     source          1..9048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             171..439
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     intron          479..575
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      1803..1812
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1809..1811,1812..1814,1815..2528)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1809..1811,1812..1814,1815..2525)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1854..1875)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2115..2134)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2462..2483
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2711..2730)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     promoter        complement(3059..3077)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3098..3114)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3098..3114)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3111..3133)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3122..3138
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3146..3152,3153..3170,3171..3176))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3191..3212
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3329..3346)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3500..4088)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3580..3599)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4259..5050,5051..5119))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4882..4901
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5120..5224)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5250..5705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5387..5408)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5599..5618
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5832..5854
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5846..5863
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5847..5863
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5870..5889
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5870..5888
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5925..6059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5949..5968)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6003..6022
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6484..6504)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6634..6699
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(7119..7913)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7230..7249)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7840..7859
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(8270..8599)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8284..8419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8642..8664)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8805..8824
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(8874..9048,1..155,156..170,171..439)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
ORIGIN
        1 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
       61 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      121 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cctcgatcga ggggctcgca
      181 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      241 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      301 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      361 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      421 ttctgcgccg ttacagatcc aagctcctcg aggaactgaa aaaccagaaa gttaactggt
      481 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag
      541 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      601 tctaaaagct gcggaattcc ggttccttgg tgtatgtttt gctgcaatca ttagagcctg
      661 agtcactctc cccaggagac ccagacctag aactacccag agcaagacca cagctggtga
      721 acagtccagg agcagacaag atggagacaa attcctctct ccccacgaac atctctggag
      781 ggacacctgc tgtatctgct ggctatctct tcctggatat catcacttat ctggtatttg
      841 cagtcacctt tgtcctcggg gtcctgggca acgggcttgt gatctgggtg gctggattcc
      901 ggatgacaca cacagtcacc accatcagtt acctgaacct ggccgtggct gacttctgtt
      961 tcacctccac tttgccattc ttcatggtca ggaaggccat gggaggacat tggcctttcg
     1021 gctggttcct gtgcaaattc gtctttacca tagtggacat caacttgttc ggaagtgtct
     1081 tcctgatcgc cctcattgct ctggaccgct gtgtttgcgt cctgcatcca gtctggaccc
     1141 agaaccaccg caccgtgagc ctggccaaga aggtgatcat tgggccctgg gtgatggctc
     1201 tgctcctcac attgccagtt atcattcgtg tgactacagt acctggtaaa acggggacag
     1261 tagcctgcac ttttaacttt tcgccctgga ccaacgaccc taaagagagg ataaatgtgg
     1321 ccgttgccat gttgacggtg agaggcatca tccggttcat cattggcttc agcgcaccca
     1381 tgtccatcgt tgctgtcagt tatgggctta ttgccaccaa gatccacaag caaggcttga
     1441 ttaagtccag tcgtccctta cgggtcctct cctttgtcgc agcagccttt tttctctgct
     1501 ggtccccata tcaggtggtg gcccttatag ccacagtcag aatccgtgag ttattgcaag
     1561 gcatgtacaa agaaattggt attgcagtgg atgtgacaag tgccctggcc ttcttcaaca
     1621 gctgcctcaa ccccatgctc tatgtcttca tgggccagga cttccgggag aggctgatcc
     1681 acgcccttcc cgccagtctg gagagggccc tgaccgagga ctcaacccaa accagtgaca
     1741 cagctaccaa ttctacttta ccttctgcag aggtggagtt acaggcaaag gatccaccgg
     1801 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1861 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1921 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1981 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     2041 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2101 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2161 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2221 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2281 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2341 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2401 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2461 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2521 acaagtaaag cggccgcgac tctagacctg gccttggaag ttgccactcc agtgcccacc
     2581 agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc cttctataat
     2641 attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac aacctgtagg
     2701 gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg gctcactgca
     2761 atctccgcct cctgggttca agcgattccc ctgcctcagc ctcccgagtt gttgggattc
     2821 caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg gggtttcacc
     2881 atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc ttggcctccc
     2941 aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct gattttaaaa
     3001 taactatacc agcaggagga cgtccagaca cagcataggc gagctccagc ttttgttccc
     3061 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     3121 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     3181 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     3241 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     3301 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3361 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3421 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3481 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3541 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3601 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3661 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3721 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3781 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3841 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3901 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3961 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4021 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4081 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4141 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4201 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4261 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4321 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4381 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4441 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4501 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4561 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4621 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4681 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     4741 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     4801 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     4861 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4921 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4981 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5041 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5101 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5161 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5221 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     5281 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     5341 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     5401 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     5461 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     5521 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     5581 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     5641 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     5701 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     5761 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     5821 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     5881 actatagggc gaattgggta ccgggccccc cctcgaggtc gaccgatcca gacatgataa
     5941 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6001 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     6061 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     6121 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag tcaaggcact
     6181 atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg tacacaattt
     6241 ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa aaaacagtat
     6301 gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt ccataatttt
     6361 cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag caagagttct
     6421 attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt ccttggggtc
     6481 ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag tagcctcatc
     6541 atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg cattccacca
     6601 ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa ttagaatcag
     6661 tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt aaatctctgt
     6721 aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa agatccggac
     6781 caaagcggcc atcgtgcctc cccactcctg cagttcgggg gcatggatgc gcggatagcc
     6841 gctgctggtt tcctggatgc cgacggattt gcactgccgg tagaactccg cgaggtcgtc
     6901 cagcctcagg cagcagctga accaactcgc gaggggatcg agcccggggt gggcgaagaa
     6961 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     7021 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     7081 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     7141 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     7201 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     7261 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     7321 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     7381 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     7441 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     7501 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     7561 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     7621 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     7681 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     7741 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     7801 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     7861 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     7921 gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc
     7981 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     8041 ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc
     8101 ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag
     8161 tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta cgtgttccgc
     8221 ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagcttt ttgcaaaagc
     8281 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     8341 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     8401 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     8461 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
     8521 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     8581 cacaccctaa ctgacacaca ttccacagct gcctcgcgcg tttcggtgat gacggtgaaa
     8641 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8701 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     8761 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     8821 tgtactgaga gtgcaccata ggtcgacggt atcgataagc ttggctgtgg aatgtgtgtc
     8881 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8941 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     9001 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactc
//
