LOCUS       sequence-257364-       11044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11044)
  AUTHORS   Liu Y, Bai C, Liu Q, Xu Q, Qian Z, Peng Q, Yu J, Xu M, Zhou X, Zhang
            Y, Cai M
  TITLE     Engineered ethanol-driven biosynthetic system for improving 
            production of acetyl-CoA derived drugs in Crabtree-negative yeast.
  JOURNAL   Metab Eng. 2019 Jul;54:275-284. doi: 10.1016/j.ymben.2019.05.001. 
            Epub 2019 May 9.
  PUBMED    31077813
REFERENCE   2  (bases 1 to 11044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-07"; volume: "54:275-284. doi"; pages: " 
            10.1016/j.ymben.2019.05.001. Epub 2019 May 9"
COMMENT     Sequence Label: pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*
FEATURES             Location/Qualifiers
     source          1..11044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        367..843
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             3013..3042
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3058..3075
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3172..3418
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3419..3895
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             4940..4957
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5054..5300
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(6125..6940)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6125..6940)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6848..6867
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6932..6951
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(7105..7124)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    7319..8067
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(8102..8124)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8210..8350
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8265..8284
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8365..8382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8536..9124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8616..8635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9295..10086,10087..10155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9918..9937
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10156..10260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10328..10346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(10467..11044,1..358)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
ORIGIN
        1 cattaatgct tagcgcagtc tctctatcgc ttctgaaccc cggtgcacct gtgccgaaac
       61 gcaaatgggg aaacacccgc tttttggatg attatgcatt gtctccacat tgtatgcttc
      121 caagattctg gtgggaatac tgctgatagc ctaacgttca tgatcaaaat ttaactgttc
      181 taacccctac ttgacagcaa tatataaaca gaaggaagct gccctgtctt aaaccttttt
      241 ttttatcatc attattagct tactttcata attgcgactg gttccaattg acaagctttt
      301 gattttaacg acttttaacg acaacttgag aagatcaaaa aacaactaat tattcgaaga
      361 ctagtctttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
      421 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
      481 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
      541 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
      601 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
      661 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
      721 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
      781 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
      841 tatatgtcgc cctctgccgt acaatcatca aaactagaag aacagtcaag tgaaattgac
      901 aagttgaaag caaaaatgtc ccagtctgcc tccactgcgc agcagaagaa ggaacatgag
      961 tatgaacatt tgacctcggt caagatcgtg ccacaacggc ccatctcaga tagactgcag
     1021 cccgcaattg ctacccacta ttctccacac ttggacgggt tgcaggacta tcagcgcttg
     1081 cacaaggagt ctattgaaga ccctgctaag ttcttcggtt ctaaagctac ccaattttta
     1141 aactggtcta agccattcga taaggtgttc atcccagact ctaaaacggg taggccctcc
     1201 ttccagaaca atgcatggtt cctcaacggc caattaaacg cctgttacaa ctgtgttgac
     1261 agacatgcct tgaagacccc taacaagaaa gccattattt tcgaaggtga cgagcctggc
     1321 caaggctatt ccattaccta caaggaacta cttgaagaag tttgtcaagt ggcacaagtg
     1381 ctgacttact ctatgggcgt tcgcaagggc gatactgttg ccgtgtacat gcctatggtc
     1441 ccagaagcaa tcataacctt gttggccatt tcccgtatcg gcgccattca ctccgtagtc
     1501 tttgccgggt tttcttccaa ctccttgaga gatcgtatca acgatgggga ctctaaagtt
     1561 gtcatcacta cagatgaatc caacagaggt ggtaaagtca ttgagactaa aagaattgtt
     1621 gatgacgcgc taagagagac cccaggcgtg agacacgtct tggtttatag aaagaccaac
     1681 aatccatctg ttgctttcca tgcccccaga gatttagatt gggcaacaga aaagaagaaa
     1741 tacaagacct actatccatg cacacccgtt gattctgagg atccattatt cttgttgtat
     1801 acgtctggtt ctactggtgc ccccaagggt gttcaacatt ctaccgcagg ttacttgctg
     1861 ggagctttgt tgaccatgcg ctacactttt gacactcacc aagaagacgt tttcttcaca
     1921 gctggagaca ttggctggat tacaggccac acttatgtgg tttatggtcc cttactatat
     1981 ggttgtgcca ctttggtctt tgaagggact cctgcgtacc caaattactc ccgttattgg
     2041 gatattattg atgaacacaa agtcacccaa ttttatgttg ccccaactgc tttgcgtttg
     2101 ttgaaaagag ctggtgattc ctacatcgaa aatcattcct taaaatcttt gcgttgcttg
     2161 ggttcggtcg gtgaaccaat tgctgctgaa gtttgggagt ggtactctga aaaaataggt
     2221 aaaaatgaaa tccccattgt agacacctac tggcaaacag aatctggttc gcatctggtc
     2281 accccgctgg ctggtggtgt cacaccaatg aaaccgggtt ctgcctcatt ccccttcttc
     2341 ggtattgatg cagttgttct tgaccctaac actggtgaag aacttaatac cagccacgca
     2401 gagggtgtcc ttgccgtcaa agctgcatgg ccatcatttg caagaactat ttggaaaaat
     2461 catgataggt atctagacac ttatttgaac ccttaccctg gctactattt cactggtgat
     2521 ggtgctgcaa aggataagga tggttatatc tggattttgg gtcgtgtaga cgatgtggtg
     2581 aacgtctctg gtcaccgtct gtctaccgct gaaattgagg ctgctattat cgaagatcca
     2641 attgtggccg agtgtgctgt tgtcggattc aacgatgact tgactggtca agcagttgct
     2701 gcatttgtgg tgttgaaaaa caaatctaat tggtccaccg caacagatga tgaattacaa
     2761 gatatcaaga agcatttggt ctttactgtt agaaaagaca tcgggccatt tgccgcacca
     2821 aaattgatca ttttagtgga tgacttgccc aagacaagat ctggcaaaat tatgagacgt
     2881 attttaagaa aaatcctagc aggagaaagt gaccaactag gcgacgtttc tacattgtca
     2941 aaccctggca ttgttagaca tccaattgat tcggtcaagt tgcctcgagc cgcggcggcc
     3001 gccagctttc tagaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat
     3061 catcatcatc atcattgacc tagggcggcc gcgaattaat tcgccttaga catgactgtt
     3121 cctcagttca agttgggcac ttacgagaag accggtcttg ctagattcta atcaagagga
     3181 tgtcagaatg ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta
     3241 acctatatag tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg
     3301 atcagcctat ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag
     3361 tttgatgttt ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagatt
     3421 tttgtagaaa tgtcttggtg tcctcgtcca atcaggtagc catctctgaa atatctggct
     3481 ccgttgcaac tccgaacgac ctgctggcaa cgtaaaattc tccggggtaa aacttaaatg
     3541 tggagtaatg gaaccagaaa cgtctcttcc cttctctctc cttccaccgc ccgttaccgt
     3601 cgctaggaaa ttttactctg ctggagagct tcttctacgg cccccttgca gcaatgctct
     3661 tcccagcatt acgttgcggg taaaacggag gtcgtgtacc cgacctagca gcccagggat
     3721 ggaaaagtcc cggccgtcgc tggcaataat agcgggcgga cgcatgtcat gagattattg
     3781 gaaaccacca gaatcgaata taaaaggcga acacctttcc caattttggt ttctcctgac
     3841 ccaaagactt taaatttaat ttatttgtcc ctatttcaat caattgaaca actatatgtc
     3901 tattccagaa actcaaaaag ccattatctt ctacgaatcc aacggcaagt tggagcataa
     3961 ggatatccca gttccaaagc caaagcccaa cgaattgtta atcaacgtca agtactctgg
     4021 tgtctgccac accgatttgc acgcttggca tggtgactgg ccattgccaa ctaagttacc
     4081 attagttggt ggtcacgaag gtgccggtgt cgttgtcggc atgggtgaaa acgttaaggg
     4141 ctggaagatc ggtgactacg ccggtatcaa atggttgaac ggttcttgta tggcctgtga
     4201 atactgtgaa ttgggtaacg aatccaactg tcctcacgct gacttgtctg gttacaccca
     4261 cgacggttct ttccaagaat acgctaccgc tgacgctgtt caagccgctc acattcctca
     4321 aggtactgac ttggctgaag tcgcgccaat cttgtgtgct ggtatcaccg tatacaaggc
     4381 tttgaagtct gccaacttga gagcaggcca ctgggcggcc atttctggtg ctgctggtgg
     4441 tctaggttct ttggctgttc aatatgctaa ggcgatgggt tacagagtct taggtattga
     4501 tggtggtcca ggaaaggaag aattgtttac ctcgctcggt ggtgaagtat tcatcgactt
     4561 caccaaagag aaggacattg ttagcgcagt cgttaaggct accaacggcg gtgcccacgg
     4621 tatcatcaat gtttccgttt ccgaagccgc tatcgaagct tctaccagat actgtagggc
     4681 gaacggtact gttgtcttgg ttggtttgcc agccggtgca aagtgctcct ctgatgtctt
     4741 caaccacgtt gtcaagtcta tctccattgt cggctcttac gtggggaaca gagctgatac
     4801 cagagaagcc ttagatttct ttgccagagg tctagtcaag tctccaataa aggtagttgg
     4861 cttatccagt ttaccagaaa tttacgaaaa gatggagaag ggccaaattg ctggtagata
     4921 cgttgttgac acttctaaac atcatcatca tcatcattaa cctagggcgg ccgcgaatta
     4981 attcgcctta gacatgactg ttcctcagtt caagttgggc acttacgaga agaccggtct
     5041 tgctagattc taatcaagag gatgtcagaa tgccatttgc ctgagagatg caggcttcat
     5101 ttttgatact tttttatttg taacctatat agtataggat tttttttgtc attttgtttc
     5161 ttctcgtacg agcttgctcc tgatcagcct atctcgcagc tgatgaatat cttgtggtag
     5221 gggtttggga aaatcattcg agtttgatgt ttttcttggt atttcccact cctcttcaga
     5281 gtacagaaga ttaagtgaga cgttcgtttg tgcaagctta tcgataagct ttaatgcggt
     5341 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     5401 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     5461 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     5521 gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     5581 tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     5641 tacgcgatca tggcgaccac acccgtcctg tggatctatc gaatctaaat gtaagttaaa
     5701 atctctaaat aaacttgaag tcggacagtg agtgtagtct tgagaaattc tgaagccgta
     5761 tttttattat cagtgagtca gtcatcagga gatcctctac gccggacgca tcgtggccga
     5821 cctgcagggg ggggggggcg ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac
     5881 caggcctgaa tcgccccatc atccagccag aaagtgaggg agccacggtt gatgagagct
     5941 ttgttgtagg tggaccagtt ggtgattttg aacttttgct ttgccacgga acggtctgcg
     6001 ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa aagttcgatt tattcaacaa
     6061 agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt
     6121 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     6181 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     6241 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     6301 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     6361 cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact tgttcaacag
     6421 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     6481 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     6541 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     6601 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     6661 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     6721 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     6781 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     6841 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     6901 gcggcctcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     6961 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac
     7021 atcagagatt ttgagacaca acgtggcttt cccccccccc cctgcaggtc ggcatcaccg
     7081 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     7141 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     7201 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     7261 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     7321 gagtatctat gattggaagt atgggaatgg tgatacccgc attcttcagt gtcttgaggt
     7381 ctcctatcag attatgccca actaaagcaa ccggaggagg agatttcatg gtaaatttct
     7441 ctgacttttg gtcatcagta gactcgaact gtgagactat ctcggttatg acagcagaaa
     7501 tgtccttctt ggagacagta aatgaagtcc caccaataaa gaaatccttg ttatcaggaa
     7561 caaacttctt gtttcgaact ttttcggtgc cttgaactat aaaatgtaga gtggatatgt
     7621 cgggtaggaa tggagcgggc aaatgcttac cttctggacc ttcaagaggt atgtagggtt
     7681 tgtagatact gatgccaact tcagtgacaa cgttgctatt tcgttcaaac cattccgaat
     7741 ccagagaaat caaagttgtt tgtctactat tgatccaagc cagtgcggtc ttgaaactga
     7801 caatagtgtg ctcgtgtttt gaggtcatct ttgtatgaat aaatctagtc tttgatctaa
     7861 ataatcttga cgagccagac gataatacca atctaaactc tttaaacgtt aaaggacaag
     7921 tatgtctgcc tgtattaaac cccaaatcag ctcgtagtct gatcctcatc aacttgaggg
     7981 gcactatctt gttttagaga aatttgcgga gatgcgatat cgagaaaaag gtacgctgat
     8041 tttaaacgtg aaatttatct catgatctct gcctcgcgcg tttcggtgat gacggtgaaa
     8101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     8221 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     8281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     8341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     9001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10321 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattaattc tcatgtttga
    10381 cagcttatca tcgataagct gactcatgtt ggtattgtga aatagacgca gatcgggaac
    10441 actgaaaaat aacagttatt attcgagatc taacatccaa agacgaaagg ttgaatgaaa
    10501 cctttttgcc atccgacatc cacaggtcca ttctcacaca taagtgccaa acgcaacagg
    10561 aggggataca ctagcagcag accgttgcaa acgcaggacc tccactcctc ttctcctcaa
    10621 cacccacttt tgccatcgaa aaaccagccc agttattggg cttgattgga gctcgctcat
    10681 tccaattcct tctattaggc tactaacacc atgactttat tagcctgtct atcctggccc
    10741 ccctggcgag gttcatgttt gtttatttcc gaatgcaaca agctccgcat tacacccgaa
    10801 catcactcca gatgagggct ttctgagtgt ggggtcaaat agtttcatgt tccccaaatg
    10861 gcccaaaact gacagtttaa acgctgtctt ggaacctaat atgacaaaag cgtgatctca
    10921 tccaagatga actaagtttg gttcgttgaa atgctaacgg ccagttggtc aaaaagaaac
    10981 ttccaaaagt cggcataccg tttgtcttgt ttggtattga ttgacgaatg ctcaaaaata
    11041 atct
//
