LOCUS       sequence-257371-        4158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing BenR transcription factor gene under control of benzoate 
            responsive promoter (pBen) in a feedback loop..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Pandi A, Koch M, Voyvodic PL, Soudier P, Bonnet J, Kushwaha M, 
            Faulon JL
  TITLE     Metabolic perceptrons for neural computing in biological systems.
  JOURNAL   Nat Commun. 2019 Aug 28;10(1):3880. doi: 10.1038/s41467-019-11889-0.
  PUBMED    31462649
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-28"; volume: "10(1):3880. doi"; pages: " 
            10.1038/s41467-019-11889-0"
COMMENT     Sequence Label: pSB4C5_pBen-BenR
FEATURES             Location/Qualifiers
     source          1..4158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             677..1336
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(743..762)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      2563..2634
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2713..2807
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      3299..3521
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             join(3569..4158,1..361)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELTTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 tttaaacact tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac
       61 gttgattttc caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa
      121 ccagataaaa atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctaca
      181 taacggacta agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt
      241 tgaggcaaaa tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg
      301 gctcacgcaa aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg
      361 aggttcttat ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa
      421 caactgttca ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg
      481 taaaaaagat agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc
      541 atgaacagat cgacaatgta actactagag gttgatcggg cacgtaagag gttccaactt
      601 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
      661 agctaaggaa gctaaaatgg agaaaaaaat cacgggatat accaccgttg atatatccca
      721 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      781 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      841 ttatccggcc tttattcaca ttcttgcccg cctgatgaac gctcacccgg agtttcgtat
      901 ggccatgaaa gacggtgagc tggtgatctg ggatagtgtt cacccttgtt acaccgtttt
      961 ccatgagcaa actgaaacgt tttcgtccct ctggagtgaa taccacgacg atttccggca
     1021 gtttctccac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1081 taaagggttt attgagaata tgttttttgt ctcagccaat ccctgggtga gtttcaccag
     1141 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca cgatgggcaa
     1201 atattatacg caaggcgaca aggtgctgat gccgctggcg atccaggttc atcatgccgt
     1261 ttgtgatggc ttccatgtcg gccgcatgct taatgaatta caacagtact gtgatgagtg
     1321 gcagggcggg gcgtaataat actagctccg gcaaaaaaac gggcaaggtg tcaccaccct
     1381 gccctttttc tttaaaaccg aaaagattac ttcgcgtttg ccacctgacg tctaagacga
     1441 tactgttcga agcattgcca ttttctgaag ttaccgaaaa agtaccgaac atccgtaaat
     1501 ctggataacg ttctgcacaa tccggatagc ccccccgcca gccgtctccc taacctgacc
     1561 aggtctaaac aataacaagg gagagtctgg ccatggaatc tcgtctgctg tctgaacgtt
     1621 cttctgtttt ccaccacgct gacccgtacg ctgtttctga ctacgttaac cagcacgttg
     1681 gtcagcactg catcggtctg tctcgtacca cccacccgca ggcttctctg tctcaccgta
     1741 aattcgctga actggacctg tgccgtatct cttacggtgg ttctgttcgt gttacctctc
     1801 cggctctgga aaccatctac cacctgcagg ttctgctgaa cggtaactgc ctgtggcgtg
     1861 gtcacaaacg tgaacagcac ctggttccgg gtgaactgct gctgatcaac ccggacgacc
     1921 cggttgacct gacctactct gaagactgcg aaaaattcat cctgaaagtt ccgacccgtc
     1981 tgctggactc tatctgcgac gaacagcgtt ggcagcgtcc ggacggtggt gttcgtttcc
     2041 tgcgtaacca ctaccgtctg gacgaactgg acggtttcgt taacctgctg gctatggttt
     2101 gccacgaagc tgaagtttct gactctctgc cgcgtgttca gggtcactac tctcagatcg
     2161 ttgcttctaa actgctgacc ctgatgtcta ccaacatccg tcgtgaatct ctgtctgctc
     2221 cgcaggctgg tctggaacgt atcctggact acatcgaacg taacctgaaa ctggaactgt
     2281 ctgctgaagt tctggctgaa caggcttgca tgtctctgcg ttctctgtac gctctgttcg
     2341 accagcacct gggtatcacc ccgaaacact acgttcgtca gcgtaaactg gaacgtgttc
     2401 acgcttgcct gtctgacccg acctgcggtg ttcgttctgt taccgaactg gctctggact
     2461 acggtttcct gcacctgggt cgtttctctg aaatctaccg tcagcagttc ggtgaactgc
     2521 cgtctcagac cttcaaacgt cgtgcttaat agacccaggc atcaaataaa acgaaaggct
     2581 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2641 agtcacactg gctcaccttc gggtgggccg acttctgcta gagctgtcag accaagttta
     2701 cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggatt
     2761 tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca agcactaggg
     2821 acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca ttagccagtc
     2881 tgaatgacct gtcacgggat aatccgaagt ggtcagactg gaaaatcaga gggcaggaac
     2941 tgctgaacag caaaaagtca gatagcacca catagcagac ccgccataaa acgccctgag
     3001 aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat ccataaaagg
     3061 cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc gaactgaatg
     3121 tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa tattcagcga
     3181 tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc tgttttgtag
     3241 aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact aaagtagtga
     3301 gttatacaca gggctgggat ctattctttt tatctttttt tattctttct ttattctata
     3361 aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg aatttacaga
     3421 gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac agatacccac
     3481 aactcaaagg aaaaggacat gtaattatca ttgactagcc catctcaatt ggtatagtga
     3541 ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca aagcaaatga
     3601 actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt tatgctgtgt
     3661 ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg tatcgttcac
     3721 ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg gtgtattagc
     3781 taaagcaacc agagagctga cgacgagaac tgtggaaatc aggaatcctt tggttaaagg
     3841 ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat tagaattagt
     3901 ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa aatataatct
     3961 ggaacatgtt aagtcttttg aaaacaaata ctccatgagg atttatgagt ggttattaaa
     4021 agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg atgaatttaa
     4081 gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc aatgggtttt
     4141 gaaaccaata agtaaaga
//
