LOCUS       sequence-257372-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  activating MTOR mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Grabiner BC, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, Jordan
            A, Beck AH, Sabatini DM
  TITLE     A diverse array of cancer-associated MTOR mutations are 
            hyperactivating and can predict rapamycin sensitivity.
  JOURNAL   Cancer Discov. 2014 May;4(5):554-63. doi: 
            10.1158/2159-8290.CD-13-0929. Epub 2014 Mar 14.
  PUBMED    24631838
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0929"; journalName: "Cancer Discov"; date: 
            "2014-05"; volume: "4"; issue: "5"; pages: "554-63"
COMMENT     Sequence Label: pcDNA3-FLAG-MTOR-S2215Y
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..36)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        208..587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        588..791
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     742..762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     836..855
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        836..854
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      940..949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3821..3830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7030..7308
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
     CDS             7042..7314
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     primer_bind     complement(8729..8745)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8729..8745)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(8780..8798)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8781..8798)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8818..8835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8824..9048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9094..9522
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9181..9200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9391..9412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9531..9551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9536..9865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9716..9851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9778..9797
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9932..10726
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9986..10005)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10596..10615
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10900..11021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10937..10956)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10991..11010
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11070..11086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11070..11086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11083..11105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11094..11110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11118..11124,11125..11142,11143..11148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11163..11184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11301..11318)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11472..12060)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11552..11571)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12231..13022,13023..13091))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12854..12873
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13092..13196)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
       61 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      181 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      241 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      301 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      361 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      421 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      481 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      541 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      601 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      661 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      721 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      781 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
      841 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
      901 cagtgtgctg gaattctgca gatatccatc acactggcgg ccgccatgga ttataaagat
      961 gatgatgata tgcttggaac cggacctgcc gccgccacca ccgctgccac cacatctagc
     1021 aatgtgagcg tcctgcagca gtttgccagt ggcctaaaga gccggaatga ggaaaccagg
     1081 gccaaagccg ccaaggagct ccagcactat gtcaccatgg aactccgaga gatgagtcaa
     1141 gaggagtcta ctcgcttcta tgaccaactg aaccatcaca tttttgaatt ggtttccagc
     1201 tcagatgcca atgagaggaa aggtggcatc ttggccatag ctagcctcat aggagtggaa
     1261 ggtgggaatg ccacccgaat tggcagattt gccaactatc ttcggaacct cctcccctcc
     1321 aatgacccag ttgtcatgga aatggcatcc aaggccattg gccgtcttgc catggcaggg
     1381 gacactttta ccgctgagta cgtggaattt gaggtgaagc gagccctgga atggctgggt
     1441 gctgaccgca atgagggccg gagacatgca gctgtcctgg ttctccgtga gctggccatc
     1501 agcgtcccta ccttcttctt ccagcaagtg caacccttct ttgacaacat ttttgtggcc
     1561 gtgtgggacc ccaaacaggc catccgtgag ggagctgtag ccgcccttcg tgcctgtctg
     1621 attctcacaa cccagcgtga gccgaaggag atgcagaagc ctcagtggta caggcacaca
     1681 tttgaagaag cagagaaggg atttgatgag accttggcca aagagaaggg catgaatcgg
     1741 gatgatcgga tccatggagc cttgttgatc cttaacgagc tggtccgaat cagcagcatg
     1801 gagggagagc gtctgagaga agaaatggaa gaaatcacac agcagcagct ggtacacgac
     1861 aagtactgca aagatctcat gggcttcgga acaaaacctc gtcacattac ccccttcacc
     1921 agtttccagg ctgtacagcc ccagcagtca aatgccttgg tggggctgct ggggtacagc
     1981 tctcaccaag gcctcatggg atttgggacc tcccccagtc cagctaagtc caccctggtg
     2041 gagagccggt gttgcagaga cttgatggag gagaaatttg atcaggtgtg ccagtgggtg
     2101 ctgaaatgca ggaatagcaa gaactcgctg atccaaatga caatccttaa tttgttgccc
     2161 cgcttggctg cattccgacc ttctgccttc acagataccc agtatctcca agataccatg
     2221 aaccatgtcc taagctgtgt caagaaggag aaggaacgta cagcggcctt ccaagccctg
     2281 gggctacttt ctgtggctgt gaggtctgag tttaaggtct atttgcctcg cgtgctggac
     2341 atcatccgag cggccctgcc cccaaaggac ttcgcccata agaggcagaa ggcaatgcag
     2401 gtggacgcca cagtcttcac ttgcatcagc atgctggctc gagcaatggg gccaggcatc
     2461 cagcaggata tcaaggagct gctggagccc atgctggcag tgggactaag ccctgccctc
     2521 actgcagtgc tctacgacct gagccgtcag attccacagc taaagaagga cattcaagat
     2581 gggctactga aaatgctgtc cctggtcctt atgcacaaac cccttcgcca cccaggcatg
     2641 cccaagggcc tggcccatca gctggcctct cctggcctca cgaccctccc tgaggccagc
     2701 gatgtgggca gcatcactct tgccctccga acgcttggca gctttgaatt tgaaggccac
     2761 tctctgaccc aatttgttcg ccactgtgcg gatcatttcc tgaacagtga gcacaaggag
     2821 atccgcatgg aggctgcccg cacctgctcc cgcctgctca caccctccat ccacctcatc
     2881 agtggccatg ctcatgtggt tagccagacc gcagtgcaag tggtggcaga tgtgcttagc
     2941 aaactgctcg tagttgggat aacagatcct gaccctgaca ttcgctactg tgtcttggcg
     3001 tccctggacg agcgctttga tgcacacctg gcccaggcgg agaacttgca ggccttgttt
     3061 gtggctctga atgaccaggt gtttgagatc cgggagctgg ccatctgcac tgtgggccga
     3121 ctcagtagca tgaaccctgc ctttgtcatg cctttcctgc gcaagatgct catccagatt
     3181 ttgacagagt tggagcacag tgggattgga agaatcaaag agcagagtgc ccgcatgctg
     3241 gggcacctgg tctccaatgc cccccgactc atccgcccct acatggagcc tattctgaag
     3301 gcattaattt tgaaactgaa agatccagac cctgatccaa acccaggtgt gatcaataat
     3361 gtcctggcaa caataggaga attggcacag gttagtggcc tggaaatgag gaaatgggtt
     3421 gatgaacttt ttattatcat catggacatg ctccaggatt cctctttgtt ggccaaaagg
     3481 caggtggctc tgtggaccct gggacagttg gtggccagca ctggctatgt agtagagccc
     3541 tacaggaagt accctacttt gcttgaggtg ctactgaatt ttctgaagac tgagcagaac
     3601 cagggtacac gcagagaggc catccgtgtg ttagggcttt taggggcttt ggatccttac
     3661 aagcacaaag tgaacattgg catgatagac cagtcccggg atgcctctgc tgtcagcctg
     3721 tcagaatcca agtcaagtca ggattcctct gactatagca ctagtgaaat gctggtcaac
     3781 atgggaaact tgcctctgga tgagttctac ccagctgtgt ccatggtggc cctgatgcgg
     3841 atcttccgag accagtcact ctctcatcat cacaccatgg ttgtccaggc catcaccttc
     3901 atcttcaagt ccctgggact caaatgtgtg cagttcctgc cccaggtcat gcccacgttc
     3961 cttaatgtca ttcgagtctg tgatggggcc atccgggaat ttttgttcca gcagctggga
     4021 atgttggtgt cctttgtgaa gagccacatc agaccttata tggatgaaat agtcaccctc
     4081 atgagagaat tctgggtcat gaacacctca attcagagca cgatcattct tctcattgag
     4141 caaattgtgg tagctcttgg gggtgaattt aagctctacc tgccccagct gatcccacac
     4201 atgctgcgtg tcttcatgca tgacaacagc ccaggccgca ttgtctctat caagttactg
     4261 gctgcaatcc agctgtttgg cgccaacctg gatgactacc tgcatttact gctgcctcct
     4321 attgttaagt tgtttgatgc ccctgaagct ccactgccat ctcgaaaggc agcgctagag
     4381 actgtggacc gcctgacgga gtccctggat ttcactgact atgcctcccg gatcattcac
     4441 cctattgttc gaacactgga ccagagccca gaactgcgct ccacagccat ggacacgctg
     4501 tcttcacttg tttttcagct ggggaagaag taccaaattt tcattccaat ggtgaataaa
     4561 gttctggtgc gacaccgaat caatcatcag cgctatgatg tgctcatctg cagaattgtc
     4621 aagggataca cacttgctga tgaagaggag gatcctttga tttaccagca tcggatgctt
     4681 aggagtggcc aaggggatgc attggctagt ggaccagtgg aaacaggacc catgaagaaa
     4741 ctgcacgtca gcaccatcaa cctccaaaag gcctggggcg ctgccaggag ggtctccaaa
     4801 gatgactggc tggaatggct gagacggctg agcctggagc tgctgaagga ctcatcatcg
     4861 ccctccctgc gctcctgctg ggccctggca caggcctaca acccgatggc cagggatctc
     4921 ttcaatgctg catttgtgtc ctgctggtct gaactgaatg aagatcaaca ggatgagctc
     4981 atcagaagca tcgagttggc cctcacctca caagacatcg ctgaagtcac acagaccctc
     5041 ttaaacttgg ctgaattcat ggaacacagt gacaagggcc ccctgccact gagagatgac
     5101 aatggcattg ttctgctggg tgagagagct gccaagtgcc gagcatatgc caaagcacta
     5161 cactacaaag aactggagtt ccagaaaggc cccacccctg ccattctaga atctctcatc
     5221 agcattaata ataagctaca gcagccggag gcagcggccg gagtgttaga atatgccatg
     5281 aaacactttg gagagctgga gatccaggct acctggtatg agaaactgca cgagtgggag
     5341 gatgcccttg tggcctatga caagaaaatg gacaccaaca aggacgaccc agagctgatg
     5401 ctgggccgca tgcgctgcct cgaggccttg ggggaatggg gtcaactcca ccagcagtgc
     5461 tgtgaaaagt ggaccctggt taatgatgag acccaagcca agatggcccg gatggctgct
     5521 gcagctgcat ggggtttagg tcagtgggac agcatggaag aatacacctg tatgatccct
     5581 cgggacaccc atgatggggc attttataga gctgtgctgg cactgcatca ggacctcttc
     5641 tccttggcac aacagtgcat tgacaaggcc agggacctgc tggatgctga attaactgca
     5701 atggcaggag agagttacag tcgggcatat ggggccatgg tttcttgcca catgctgtcc
     5761 gagctggagg aggttatcca gtacaaactt gtccccgagc gacgagagat catccgccag
     5821 atctggtggg agagactgca gggctgccag cgtatcgtag aggactggca gaaaatcctt
     5881 atggtgcggt cccttgtggt cagccctcat gaagacatga gaacctggct caagtatgca
     5941 agcctgtgcg gcaagagtgg caggctggct cttgctcata aaactttagt gttgctcctg
     6001 ggagttgatc cgtctcggca acttgaccat cctctgccaa cagttcaccc tcaggtgacc
     6061 tatgcctaca tgaaaaacat gtggaagagt gcccgcaaga tcgatgcctt ccagcacatg
     6121 cagcattttg tccagaccat gcagcaacag gcccagcatg ccatcgctac tgaggaccag
     6181 cagcataagc aggaactgca caagctcatg gcccgatgct tcctgaaact tggagagtgg
     6241 cagctgaatc tacagggcat caatgagagc acaatcccca aagtgctgca gtactacagc
     6301 gccgccacag agcacgaccg cagctggtac aaggcctggc atgcgtgggc agtgatgaac
     6361 ttcgaagctg tgctacacta caaacatcag aaccaagccc gcgatgagaa gaagaaactg
     6421 cgtcatgcca gcggggccaa catcaccaac gccaccactg ccgccaccac ggccgccact
     6481 gccaccacca ctgccagcac cgagggcagc aacagtgaga gcgaggccga gagcaccgag
     6541 aacagcccca ccccatcgcc gctgcagaag aaggtcactg aggatctgtc caaaaccctc
     6601 ctgatgtaca cggtgcctgc cgtccagggc ttcttccgtt ccatctcctt gtcacgaggc
     6661 aacaacctcc aggatacact cagagttctc accttatggt ttgattatgg tcactggcca
     6721 gatgtcaatg aggccttagt ggagggggtg aaagccatcc agattgatac ctggctacag
     6781 gttatacctc agctcattgc aagaattgat acgcccagac ccttggtggg acgtctcatt
     6841 caccagcttc tcacagacat tggtcggtac cacccccagg ccctcatcta cccactgaca
     6901 gtggcttcta agtctaccac gacagcccgg cacaatgcag ccaacaagat tctgaagaac
     6961 atgtgtgagc acagcaacac cctggtccag caggccatga tggtgagcga ggagctgatc
     7021 cgagtggcca tcctctggca tgagatgtgg catgaaggcc tggaagaggc atctcgtttg
     7081 tactttgggg aaaggaacgt gaaaggcatg tttgaggtgc tggagccctt gcatgctatg
     7141 atggaacggg gcccccagac tctgaaggaa acatccttta atcaggccta tggtcgagat
     7201 ttaatggagg cccaagagtg gtgcaggaag tacatgaaat cagggaatgt caaggacctc
     7261 acccaagcct gggacctcta ttatcatgtg ttccgacgaa tctcaaagca gctgcctcag
     7321 ctcacatcct tagagctgca atatgtttcc ccaaaacttc tgatgtgccg ggaccttgaa
     7381 ttggctgtgc caggaacata tgaccccaac cagccaatca ttcgcattca gtccatagca
     7441 ccgtctttgc aagtcatcac atccaagcag aggccccgga aattgacact tatgggcagc
     7501 aacggacatg agtttgtttt ccttctaaaa ggccatgaag atctgcgcca ggatgagcgt
     7561 gtgatgcagc tcttcggcct ggttaacacc cttctggcca atgacccaac atatcttcgg
     7621 aaaaacctca gcatccagag atacgctgtc atccctttat cgaccaactc gggcctcatt
     7681 ggctgggttc cccactgtga cacactgcac gccctcatcc gggactacag ggagaagaag
     7741 aagatccttc tcaacatcga gcatcgcatc atgttgcgga tggctccgga ctatgaccac
     7801 ttgactctga tgcagaaggt ggaggtgttt gagcatgccg tcaataatac agctggggac
     7861 gacctggcca agctgctgtg gctgaaaagc cccagctccg aggtgtggtt tgaccgaaga
     7921 accaattata cccgttcttt agcggtcatg tcaatggttg ggtatatttt aggcctggga
     7981 gatagacacc catccaacct gatgctggac cgtctgagtg ggaagatcct gcacattgac
     8041 tttggggact gctttgaggt tgctatgacc cgagagaagt ttccagagaa gattccattt
     8101 agactaacaa gaatgttgac caatgctatg gaggttacag gcctggatgg caactacaga
     8161 atcacatgcc acacagtgat ggaggtgctg cgagagcaca aggacagtgt catggccgtg
     8221 ctggaagcct ttgtctatga ccccttgctg aactggaggc tgatggacac aaataccaaa
     8281 ggcaacaagc gatcccgaac gaggacggat tcctactctg ctggccagtc agtcgaaatt
     8341 ttggacggtg tggaacttgg agagccagcc cataagaaaa cggggaccac agtgccagaa
     8401 tctattcatt ctttcattgg agacggtttg gtgaaaccag aggccctaaa taagaaagct
     8461 atccagatta ttaacagggt tcgagataag ctcactggtc gggacttctc tcatgatgac
     8521 actttggatg ttccaacgca agttgagctg ctcatcaaac aagcgacatc ccatgaaaac
     8581 ctctgccagt gctatattgg ctggtgccct ttctggtaac tggaggccca gatgtgccca
     8641 tcacgttttt tctgaggctt ttgtacttta gtaaatgctt ccactaaact gaaaccatgg
     8701 tgagaaagtt tgactttgtt aaatggggga tccactagtt ctagagcggc cgctcgagca
     8761 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
     8821 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     8881 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     8941 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9001 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9061 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9121 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9181 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9241 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9301 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9361 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     9421 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     9481 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     9541 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     9721 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9781 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     9841 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     9901 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     9961 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    10021 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    10081 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    10141 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    10201 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10261 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10321 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10381 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10441 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    10501 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10561 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10621 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10681 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    10741 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    10801 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10861 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    10921 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10981 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    11041 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11101 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    11161 taatgagtga gctaactcac attaattgcg ttgcgctcac tgccagcttt ccagtcggga
    11221 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11281 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11341 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11401 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11461 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11521 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11581 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11641 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11701 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11761 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    11821 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    11881 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    11941 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12001 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12061 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12121 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12181 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12241 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12301 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12361 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12421 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12481 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12541 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12601 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12661 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12721 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12781 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    12841 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    12901 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    12961 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13021 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13081 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13141 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13201 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccc
//
