LOCUS       sequence-257375-        6473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6473)
  AUTHORS   Fujita N, Jaye DL, Kajita M, Geigerman C, Moreno CS, Wade PA
  TITLE     MTA3, a Mi-2/NuRD complex subunit, regulates an invasive growth 
            pathway in breast cancer.
  JOURNAL   Cell. 2003 Apr 18. 113(2):207-19.
  PUBMED    12705869
REFERENCE   2  (bases 1 to 6473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2003 
            Apr 18. 113(2):207-19."
COMMENT     Sequence Label: Slug_pGL2
FEATURES             Location/Qualifiers
     source          1..6473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    21..142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             1118..2770
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1184..1202)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3011..3076
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3206..3226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3651..3785
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3927..3944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4098..4686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4178..4197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4857..5648,5649..5717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5480..5499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5718..5822)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5849..6304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5936..5955)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6146..6167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
       61 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
      121 ccaaactcat caatgtatct tatggtactg taactgagct aacataaccc gggaggtacc
      181 ttgtctctaa agacccatac aacccttttt cccataaaaa aaaaagatgc actgtaatac
      241 atgaaaagat aagatctctt gtcaaaagtg tgagagaatg tccggtggtt ccaaatgaca
      301 gttacctctt gccccccttc tctgccagag ttccttttta tctttgcaat cttccagttc
      361 ttccgatcag cctgccttta gagggctaca aagcatttct ttcaagccac catagctaac
      421 acggtgacat gagtacttaa tttgcacgcg gccgcgctgc ccctggcttc gcggaagccc
      481 tgagtagcgc agcgccctcg ccgcacgcaa ggctgcagtc ccgctccagg ccagagtccc
      541 aggagagcgt cctccgcgct cacaggcgcc tttgtcttcc cgcttccccc ttcctttttc
      601 aaaagccaag aggtaattat ttggtctttg tgcaaggcaa acctctccag atgccacttc
      661 caaatatagg ctctcattaa caccagaggc tggcctggtg tggtgcaggg cggcccttcc
      721 ttctcctggc ggacactgtg tccccgcgcg ctggcgctgc accacatctg gaagccaggc
      781 gggcagggca gagaccccgg ctcctgcgcc cctcctagct cccagagagc gtggatcgcg
      841 ggcggggctc accgagcgag gttacctctc ttgaaaatac ttaaacactt tttttcctct
      901 ccactgaaat ctcaaaaaac agcccatttt gaaccagaat aatttagtct gacaacagat
      961 tcttcctctg ttcacagctg tcccagaggg aggagctgaa atctgaacct ctcagctgtg
     1021 attggatctt tcttgcaaaa gagaggaaaa aaaaaccctc ccagccaaaa cgggctcagt
     1081 tcgtaaagaa gcttggcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa
     1141 gaaaggcccg gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa
     1201 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     1261 ggtgaacatc acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa
     1321 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1381 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     1441 ttataatgaa cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt
     1501 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa
     1561 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     1621 cacatctcat ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg
     1681 tgacaaaaca attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt
     1741 ggcccttccg catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg
     1801 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     1861 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     1921 tgaagaagag ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt
     1981 accaacccta ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     2041 tttacacgaa attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc
     2101 aaaacgcttc catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc
     2161 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     2221 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg
     2281 cgaattatgt gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac
     2341 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     2401 agacgaacac ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca
     2461 ggtggccccc gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg
     2521 cgtggcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     2581 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     2641 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     2701 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc
     2761 caaattgtaa aatgtaactg tattcagcga tgacgaaatt cttagctatt gtaatactgc
     2821 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
     2881 ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gccggatctt
     2941 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     3001 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     3061 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     3121 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     3181 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     3241 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     3301 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     3361 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     3421 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     3481 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     3541 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     3601 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     3661 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3721 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3781 ggatccgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     3841 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     3901 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4501 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5161 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5461 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5821 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     5881 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5941 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     6001 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     6061 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     6121 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     6181 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     6241 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     6301 atttgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6361 tcgctattac gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt
     6421 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaa
//
