LOCUS       sequence-257384-        7885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7885)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 7885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..7885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    3787..3839
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      3849..3858
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3855..3857,3858..3860,3861..4574)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3900..3921)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4161..4180)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4508..4529
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4696..4817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4733..4752)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4787..4806
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4824..5279)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4961..4982)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5173..5192
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5306..5410
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5412..5769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5412..5603
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5435..5455)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5620..5755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5804..6598
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5804..6598
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5858..5877)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6468..6487
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6786..6805)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6830..6877
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7206..7794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7695..7714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
      601 aaccgtcaga tccgctagtc gacggtacct cggcgatggc ttttccgccg cggcgacggc
      661 tgcgcctcgg tccccgcggc ctcccgcttc ttctctcggg actcctgcta cctctgtgcc
      721 gcgccttcaa cctagacgtg gacagtcctg ccgagtactc tggccccgag ggaagttact
      781 tcggcttcgc cgtggatttc ttcgtgccca gcgcgtcttc ccggatgttt cttctcgtgg
      841 gagctcccaa agcaaacacc acccagcctg ggattgtgga aggagggcag gtcctcaaat
      901 gtgactggtc ttctacccgc cggtgccagc caattgaatt tgatgcaaca ggcaatagag
      961 attatgccaa ggatgatcca ttggaattta agtcccatca gtggtttgga gcatctgtga
     1021 ggtcgaaaca ggataaaatt ttggcctgtg ccccattgta ccattggaga actgagatga
     1081 aacaggagcg agagcctgtt ggaacatgct ttcttcaaga tggaacaaag actgttgagt
     1141 atgctccatg tagatcacaa gatattgatg ctgatggaca gggattttgt caaggaggat
     1201 tcagcattga ttttactaaa gctgacagag tacttcttgg tggtcctggt agcttttatt
     1261 ggcaaggtca gcttatttcg gatcaagtgg cagaaatcgt atctaaatac gaccccaatg
     1321 tttacagcat caagtataat aaccaattag caactcggac tgcacaagct atttttgatg
     1381 acagctattt gggttattct gtggctgtcg gagatttcaa tggtgatggc atagatgact
     1441 ttgtttcagg agttccaaga gcagcaagga ctttgggaat ggtttatatt tatgatggga
     1501 agaacatgtc ctccttatac aattttactg gcgagcagat ggctgcatat ttcggatttt
     1561 ctgtagctgc cactgacatt aatggagatg attatgcaga tgtgtttatt ggagcacctc
     1621 tcttcatgga tcgtggctct gatggcaaac tccaagaggt ggggcaggtc tcagtgtctc
     1681 tacagagagc ttcaggagac ttccagacga caaagctgaa tggatttgag gtctttgcac
     1741 ggtttggcag tgccatagct cctttgggag atctggacca ggatggtttc aatgatattg
     1801 caattgctgc tccatatggg ggtgaagata aaaaaggaat tgtttatatc ttcaatggaa
     1861 gatcaacagg cttgaacgca gtcccatctc aaatccttga agggcagtgg gctgctcgaa
     1921 gcatgccacc aagctttggc tattcaatga aaggagccac agatatagac aaaaatggat
     1981 atccagactt aattgtagga gcttttggtg tagatcgagc tatcttatac agggccagac
     2041 cagttatcac tgtaaatgct ggtcttgaag tgtaccctag cattttaaat caagacaata
     2101 aaacctgctc actgcctgga acagctctca aagtttcctg ttttaatgtt aggttctgct
     2161 taaaggcaga tggcaaagga gtacttccca ggaaacttaa tttccaggtg gaacttcttt
     2221 tggataaact caagcaaaag ggagcaattc gacgagcact gtttctctac agcaggtccc
     2281 caagtcactc caagaacatg actatttcaa gggggggact gatgcagtgt gaggaattga
     2341 tagcgtatct gcgggatgaa tctgaattta gagacaaact cactccaatt actattttta
     2401 tggaatatcg gttggattat agaacagctg ctgatacaac aggcttgcaa cccattctta
     2461 accagttcac gcctgctaac attagtcgac aggctcacat tctacttgac tgtggtgaag
     2521 acaatgtctg taaacccaag ctggaagttt ctgtagatag tgatcaaaag aagatctata
     2581 ttggggatga caaccctctg acattgattg ttaaggctca gaatcaagga gaaggtgcct
     2641 acgaagctga gctcatcgtt tccattccac tgcaggctga tttcatcggg gttgtccgaa
     2701 acaatgaagc cttagcaaga ctttcctgtg catttaagac agaaaaccaa actcgccagg
     2761 tggtatgtga ccttggaaac ccaatgaagg ctggaactca actcttagct ggtcttcgtt
     2821 tcagtgtgca ccagcagtca gagatggata cttctgtgaa atttgactta caaatccaaa
     2881 gctcaaatct atttgacaaa gtaagcccag ttgtatctca caaagttgat cttgctgttt
     2941 tagctgcagt tgagataaga ggagtctcga gtcctgatca tatctttctt ccgattccaa
     3001 actgggagca caaggagaac cctgagactg aagaagatgt tgggccagtt gttcagcaca
     3061 tctatgagct gagaaacaat ggtccaagtt cattcagcaa ggcaatgctc catcttcagt
     3121 ggccttacaa atataataat aacactctgt tgtatatcct tcattatgat attgatggac
     3181 caatgaactg cacttcagat atggagatca accctttgag aattaagatc tcatctttgc
     3241 aaacaactga aaagaatgac acggttgccg ggcaaggtga gcgggaccat ctcatcacta
     3301 agcgggatct tgccctcagt gaaggagata ttcacacttt gggttgtgga gttgctcagt
     3361 gcttgaagat tgtctgccaa gttgggagat tagacagagg aaagagtgca atcttgtacg
     3421 taaagtcatt actgtggact gagactttta tgaataaaga aaatcagaat cattcctatt
     3481 ctctgaagtc gtctgcttca tttaatgtca tagagtttcc ttataagaat cttccaattg
     3541 aggatatcac caactccaca ttggttacca ctaatgtcac ctggggcatt cagccagcgc
     3601 ccatgcctgt gcctgtgtgg gtgatcattt tagcagttct agcaggattg ttgctactgg
     3661 ctgttttggt atttgtaatg tacaggatgg gcttttttaa acgggtccgg ccacctcaag
     3721 aagaacaaga aagggagcag cttcaacctc atgaaaatgg tgaaggaaac tcagaaactc
     3781 cgggatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatccc
     3841 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3901 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3961 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4021 tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc gcccgctacc
     4081 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4141 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4201 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4261 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     4321 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     4381 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4441 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     4501 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4561 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     4621 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4681 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4741 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4801 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4861 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4921 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4981 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5041 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     5101 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5161 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5221 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5281 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5341 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5401 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5461 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5521 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5581 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5641 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5701 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5761 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5821 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5881 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5941 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     6001 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6061 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6121 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6181 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6241 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6301 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6361 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6421 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6481 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6541 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6601 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6661 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6721 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6781 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6841 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6901 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6961 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     7021 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     7081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7141 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7201 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7261 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7321 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7381 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7441 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7501 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7561 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7621 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7681 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7741 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7801 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7861 cctgcgttat cccctgattc tgtgg
//
