LOCUS       sequence-257439-        1160 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His6-Ubc9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW
  TITLE     Lysine acylation using conjugating enzymes for site-specific 
            modification and ubiquitination of recombinant proteins.
  JOURNAL   Nat Chem. 2020 Nov;12(11):1008-1015. doi: 10.1038/s41557-020-0528-y.
            Epub 2020 Sep 14.
  PUBMED    32929246
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-020-0528-y"; journalName: "Nat Chem"; date: 
            "2020-11"; volume: "12"; issue: "11"; pages: "1008-1015"
COMMENT     Sequence Label: pET28-His6-Ubc9
FEATURES             Location/Qualifiers
     source          1..1160
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..56
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..83
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             573..590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(643..661)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      657..704
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      741..1121
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(828..847)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1038..1059
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtttaacttt aagaaggaga tataccatgg gcagcagcca tcatcatcat catcacagca
       61 gcggcctggt gccgcgcggc agccatatga tgtccggaat tgccctctct cgcttagctc
      121 aggaacgcaa agcgtggcgc aaagatcacc cgtttgggtt tgttgccgtg cctaccaaaa
      181 atcctgacgg tacgatgaat cttatgaact gggaatgcgc gatccctggc aagaaaggca
      241 caccgtggga aggcggcttg ttcaagctgc gtatgttgtt caaagatgat tacccgtcaa
      301 gtccgcctaa atgtaaattc gagccgccgt tatttcatcc aaacgtgtat cccagcggta
      361 cggtttgcct gagcatcctt gaagaagaca aagattggcg tcccgcaatt accatcaagc
      421 agattctgct cggcattcaa gagctgttaa atgaaccaaa cattcaggac ccagcgcagg
      481 cagaagccta tactatctac tgtcagaacc gtgtagagta tgagaagcgc gtccgtgcac
      541 aagcgaaaaa gtttgccccg tcgtaactcg agcaccacca ccaccaccac tgagatccgg
      601 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
      661 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
      721 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
      781 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
      841 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
      901 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
      961 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1021 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1081 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     1141 gctgatttaa caaaatttaa
//
