LOCUS       sequence-257450-         627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone
            for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Acharya S, Mishra A, Paul D, Ansari AH, Azhar M, Kumar M, Rauthan R,
            Sharma N, Aich M, Sinha D, Sharma S, Jain S, Ray A, Jain S, 
            Ramalingam S, Maiti S, Chakraborty D
  TITLE     Francisella novicida Cas9 interrogates genomic DNA with very high 
            specificity and can be used for mammalian genome editing.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Oct 15;116(42):20959-20968. doi: 
            10.1073/pnas.1818461116. Epub 2019 Sep 30.
  PUBMED    31570623
REFERENCE   2  (bases 1 to 627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1818461116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-10-15- 15"; volume: "116"; issue: "42"; pages: 
            "20959-20968"
COMMENT     Sequence Label: PX458-3xHA-FnCas9
FEATURES             Location/Qualifiers
     source          1..627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             22..102
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             109..129
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 accggtgcca ccatgggatc ctacccatac gatgttccag attacgctta cccatacgat
       61 gttccagatt acgcttaccc atacgatgtt ccagattacg ctatggcccc aaagaagaag
      121 cggaaggtcg gtatccacgg agtcccagca gccaatttta agatcctgcc tatcgcaatc
      181 gacctgggcg tcaagaatac tggcgtgttt agtgcttttt atcagaaggg gacctcactg
      241 gagagactgg acaataagaa cggaaaagtg tatgaactgt ccaaggattc ttacactctg
      301 ctgatgaaca ataggaccgc acggagacac cagaggcgag gaattgacag gaaacagctg
      361 gtgaagcgcc tgttcaaact gatctggaca gagcagctga acctggaatg ggataaggac
      421 actcagcagg ccatcagctt cctgtttaat cgacggggat tctcttttat tactgacggc
      481 tatagtcctg agtacctgaa catcgtgcca gaacaggtca aggcaatcct gatggacatt
      541 ttcgacgatt ataatggcga ggacgatctg gattcctacc tgaaactggc cacagagcaa
      601 gagagtaaga tcagcgaaat ctacaac
//
