LOCUS       sequence-257456-        8752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Target-AID expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto
            S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, 
            Nureki O, Tomita M, Aburatani H, Yachie N
  TITLE     Base editors for simultaneous introduction of C-to-T and A-to-G 
            mutations.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 
            10.1038/s41587-020-0509-0.
  PUBMED    32483365
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 
            10.1038/s41587-020-0509-0."
COMMENT     Sequence Label: pRS0035
FEATURES             Location/Qualifiers
     source          1..8752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4114..4134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4342..4407
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5041..5061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5068..5322
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     primer_bind     complement(5345..5362)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5351..5575
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5646..5662)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5646..5662)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5659..5681)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5670..5686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5694..5700,5701..5718,5719..5724))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5739..5760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5877..5894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6048..6636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6128..6147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6807..7598,7599..7667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7430..7449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7668..7772)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7851..7870)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8042..8421
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8422..8625
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8576..8596
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8667..8686
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8667..8685
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      8693..8702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8705..8725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(8750..8752,1..24,25..27,28..4101)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(8750..8752,1..4101)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gacaagaagt actccattgg gctcgctatc ggcacaaaca gcgtcggttg ggccgtcatt
       61 acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac
      121 agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggaaac ggccgaagcc
      181 acgcggctca aaagaacagc acggcgcaga tatacccgca gaaagaatcg gatctgctac
      241 ctgcaggaga tctttagtaa tgagatggct aaggtggatg actctttctt ccataggctg
      301 gaggagtcct ttttggtgga ggaggataaa aagcacgagc gccacccaat ctttggcaat
      361 atcgtggacg aggtggcgta ccatgaaaag tacccaacca tatatcatct gaggaagaag
      421 cttgtagaca gtactgataa ggctgacttg cggttgatct atctcgcgct ggcgcatatg
      481 atcaaatttc ggggacactt cctcatcgag ggggacctga acccagacaa cagcgatgtc
      541 gacaaactct ttatccaact ggttcagact tacaatcagc ttttcgaaga gaacccgatc
      601 aacgcatccg gagttgacgc caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg
      661 ctcgaaaacc tcatcgcaca gctccctggg gagaagaaga acggcctgtt tggtaatctt
      721 atcgccctgt cactcgggct gacccccaac tttaaatcta acttcgacct ggccgaagat
      781 gccaagcttc aactgagcaa agacacctac gatgatgatc tcgacaatct gctggcccag
      841 atcggcgacc agtacgcaga cctttttttg gcggcaaaga acctgtcaga cgccattctg
      901 ctgagtgata ttctgcgagt gaacacggag atcaccaaag ctccgctgag cgctagtatg
      961 atcaagcgct atgatgagca ccaccaagac ttgactttgc tgaaggccct tgtcagacag
     1021 caactgcctg agaagtacaa ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga
     1081 tacattgacg gcggagcaag ccaggaggaa ttttacaaat ttattaagcc catcttggaa
     1141 aaaatggacg gcaccgagga gctgctggta aagcttaaca gagaagatct gttgcgcaaa
     1201 cagcgcactt tcgacaatgg aagcatcccc caccagattc acctgggcga actgcacgct
     1261 atcctcaggc ggcaagagga tttctacccc tttttgaaag ataacaggga aaagattgag
     1321 aaaatcctca catttcggat accctactat gtaggccccc tcgcccgggg aaattccaga
     1381 ttcgcgtgga tgactcgcaa atcagaagag accatcactc cctggaactt cgaggaagtc
     1441 gtggataagg gggcctctgc ccagtccttc atcgaaagga tgactaactt tgataaaaat
     1501 ctgcctaacg aaaaggtgct tcctaaacac tctctgctgt acgagtactt cacagtttat
     1561 aacgagctca ccaaggtcaa atacgtcaca gaagggatga gaaagccagc attcctgtct
     1621 ggagagcaga agaaagctat cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg
     1681 aaacagctca aagaagacta tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc
     1741 ggagtggagg atcgcttcaa cgcatccctg ggaacgtatc acgatctcct gaaaatcatt
     1801 aaagacaagg acttcctgga caatgaggag aacgaggaca ttcttgagga cattgtcctc
     1861 acccttacgt tgtttgaaga tagggagatg attgaagaac gcttgaaaac ttacgctcat
     1921 ctcttcgacg acaaagtcat gaaacagctc aagaggcgcc gatatacagg atgggggcgg
     1981 ctgtcaagaa aactgatcaa tgggatccga gacaagcaga gtggaaagac aatcctggat
     2041 tttcttaagt ccgatggatt tgccaaccgg aacttcatgc agttgatcca tgatgactct
     2101 ctcaccttta aggaggacat ccagaaagca caagtttctg gccaggggga cagtcttcac
     2161 gagcacatcg ctaatcttgc aggtagccca gctatcaaaa agggaatact gcagaccgtt
     2221 aaggtcgtgg atgaactcgt caaagtaatg ggaaggcata agcccgagaa tatcgttatc
     2281 gagatggccc gagagaacca aactacccag aagggacaga agaacagtag ggaaaggatg
     2341 aagaggattg aagagggtat aaaagaactg gggtcccaaa tccttaagga acacccagtt
     2401 gaaaacaccc agcttcagaa tgagaagctc tacctgtact acctgcagaa cggcagggac
     2461 atgtacgtgg atcaggaact ggacatcaat cggctctccg actacgacgt ggatcatatc
     2521 gtgccccagt cttttctcaa agatgattct attgataata aagtgttgac aagatccgat
     2581 aaaaatagag ggaagagtga taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat
     2641 tattggcggc agctgctgaa cgccaaactg atcacacaac ggaagttcga taatctgact
     2701 aaggctgaac gaggtggcct gtctgagttg gataaagccg gcttcatcaa aaggcagctt
     2761 gttgagacac gccagatcac caagcacgtg gcccaaattc tcgattcacg catgaacacc
     2821 aagtacgatg aaaatgacaa actgattcga gaggtgaaag ttattactct gaagtctaag
     2881 ctggtctcag atttcagaaa ggactttcag ttttataagg tgagagagat caacaattac
     2941 caccatgcgc atgatgccta cctgaatgca gtggtaggca ctgcacttat caaaaaatat
     3001 cccaagcttg aatctgaatt tgtttacgga gactataaag tgtacgatgt taggaaaatg
     3061 atcgcaaagt ctgagcagga aataggcaag gccaccgcta agtacttctt ttacagcaat
     3121 attatgaatt ttttcaagac cgagattaca ctggccaatg gagagattcg gaagcgacca
     3181 cttatcgaaa caaacggaga aacaggagaa atcgtgtggg acaagggtag ggatttcgcg
     3241 acagtccgga aggtcctgtc catgccgcag gtgaacatcg ttaaaaagac cgaagtacag
     3301 accggaggct tctccaagga aagtatcctc ccgaaaagga acagcgacaa gctgatcgca
     3361 cgcaaaaaag attgggaccc caagaaatac ggcggattcg attctcctac agtcgcttac
     3421 agtgtactgg ttgtggccaa agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag
     3481 gaactgctgg gcatcacaat catggagcga tcaagcttcg aaaaaaaccc catcgacttt
     3541 ctcgaggcga aaggatataa agaggtcaaa aaagacctca tcattaagct tcccaagtac
     3601 tctctctttg agcttgaaaa cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag
     3661 aaaggtaacg agctggcact gccctctaaa tacgttaatt tcttgtatct ggccagccac
     3721 tatgaaaagc tcaaagggtc tcccgaagat aatgagcaga agcagctgtt cgtggaacaa
     3781 cacaaacact accttgatga gatcatcgag caaataagcg aattctccaa aagagtgatc
     3841 ctcgccgacg ctaacctcga taaggtgctt tctgcttaca ataagcacag ggataagccc
     3901 atcagggagc aggcagaaaa cattatccac ttgtttactc tgaccaactt gggcgcgcct
     3961 gcagccttca agtacttcga caccaccata gacagaaagc ggtacacctc tacaaaggag
     4021 gtcctggacg ccacactgat tcatcagtca attacggggc tctatgaaac aagaatcgac
     4081 ctctctcagc tcggtggaga cagcagggct gaccccaaga agaagaggaa ggtgggtgga
     4141 ggaggtaccg gcggtggagg ctcagcagaa tacgtacgag ctctgtttga cttcaatggg
     4201 aatgacgagg aggatctccc ctttaagaag ggcgatattc tccgcatcag agataagccc
     4261 gaagaacaat ggtggaatgc cgaggatagc gaagggaaaa ggggcatgat tctggtgcca
     4321 tatgtggaga aatattccgg tgactacaaa gaccatgatg gggattacaa agaccacgac
     4381 atcgactaca aagacgacga cgataaatca gggatgacag acgccgagta cgtgcgcatt
     4441 catgagaaac tggatattta caccttcaag aagcagttct tcaacaacaa gaaatctgtg
     4501 tcacaccgct gctacgtgct gtttgagttg aagcgaaggg gcgaaagaag ggcttgcttt
     4561 tggggctatg ccgtcaacaa gccccaaagt ggcaccgaga gaggaataca cgctgagata
     4621 ttcagtatcc gaaaggtgga agagtatctt cgggataatc ctgggcagtt tacgatcaac
     4681 tggtattcca gctggagtcc ttgcgctgat tgtgccgaga aaattctgga atggtataat
     4741 caggaacttc ggggaaacgg gcacacattg aaaatctggg cctgcaagct gtactacgag
     4801 aagaatgccc ggaaccagat aggactctgg aatctgaggg acaatggtgt aggcctgaac
     4861 gtgatggttt ccgagcacta tcagtgttgt cggaagattt tcatccaaag ctctcataac
     4921 cagctcaatg aaaaccgctg gttggagaaa acactgaaac gtgcggagaa gtggagatcc
     4981 gagctgagca tcatgatcca ggtcaagatt ctgcatacca ctaagtctcc agccgttggt
     5041 cccaagaaga aaagaaaagt cggtaccatg accaaccttt ccgacatcat agagaaggaa
     5101 acaggcaaac agttggtcat ccaagagtcg atactcatgc ttcctgaaga agttgaggag
     5161 gtcattggga ataagccgga aagtgacatt ctcgtacaca ctgcgtatga tgagagcacc
     5221 gatgagaacg tgatgctgct cacgtcagat gccccagagt acaaaccctg ggctctggtg
     5281 attcaggact ctaatggaga gaacaagatc aagatgctat aatgagttta aacccgctga
     5341 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5401 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5461 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5521 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5581 gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa
     5641 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5701 cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta
     5761 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5821 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5881 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5941 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6001 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6061 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6121 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6181 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6241 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6301 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6361 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6421 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6481 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6541 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6601 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6661 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6781 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6841 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6901 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6961 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7021 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7081 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7141 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7201 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7261 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7321 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7381 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7441 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7501 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7561 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7621 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7681 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7741 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7801 gtcgacggat cgggagatcg atctcccgat cccctagggt cgactctcag tacaatctgc
     7861 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     7921 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     7981 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     8041 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     8101 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     8161 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     8221 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     8281 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     8341 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     8401 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     8461 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8521 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8581 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     8641 cagatccgct agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat
     8701 ggcaccgaag aagaagcgta aagtcggaat ccacggagtt cctgcggcaa tg
//
