LOCUS       sequence-257461-       16019 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP reporter was removed in the TLCV2 vector so it could be used 
            for inducible gene CRISPR knockout in cell lines with an eGFP 
            reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16019)
  AUTHORS   Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, 
            Yu H, Shen J
  TITLE     Inducible Exoc7/Exo70 knockout reveals a critical role of the 
            exocyst in insulin-regulated GLUT4 exocytosis.
  JOURNAL   J Biol Chem. 2019 Dec 27;294(52):19988-19996. doi: 
            10.1074/jbc.RA119.010821. Epub 2019 Nov 18.
  PUBMED    31740584
REFERENCE   2  (bases 1 to 16019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010821"; journalName: "J Biol Chem"; date: 
            "2019-12-27- 27"; volume: "294"; issue: "52"; pages: "19988-19996"
COMMENT     Sequence Label: TLCV2-DelGFP
FEATURES             Location/Qualifiers
     source          1..16019
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    49..67
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    156..174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     257..281
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      310..319
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             316..4419
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             316..4419
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4420..4467
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4468..4491
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4501..4554
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     promoter        4568..4779
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4801..5397
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4801..4820)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5297..5317
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5407..5463
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5464..6081,6082..6087,6088..6123,6124..6126,6127..6162,
                     6163..6165,6166..6201,6202..6210)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6231..6819
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6284..6304)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6702..6713)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6891..7124
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7150..7167)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7156..7380
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7426..7854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7513..7532)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7723..7744
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7863..7883)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7868..8197
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8048..8183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8110..8129
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8245..8292
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8311..8685
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8815..8936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8852..8871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8906..8925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8985..9001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8985..9001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8998..9020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9009..9025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9033..9039,9040..9057,9058..9063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9078..9099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9216..9233)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9387..9975)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9467..9486)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10146..10937,10938..11006))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10769..10788
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11007..11111)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11186..11205)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11377..11756
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11758..11956
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             11974..12154
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12201..12326
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12819..13052
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13237..13281
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13579..13696
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        13747..13995
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     13747..13767
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     13918..13937
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        15877..15952
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(15989..16019,1..284)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    15997..16015
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
       61 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      121 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
      181 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
      241 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga ataccggttc
      301 tagagcgctg ccaccatgga caagaagtac agcatcggcc tggacatcgg caccaactct
      361 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
      421 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
      481 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
      541 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
      601 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
      661 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
      721 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
      781 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
      841 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
      901 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
      961 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     1021 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     1081 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     1141 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     1201 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     1261 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     1321 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     1381 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     1441 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
     1501 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     1561 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     1621 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     1681 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     1741 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     1801 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     1861 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     1921 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     1981 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     2041 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     2101 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     2161 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     2221 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     2281 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     2341 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     2401 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     2461 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     2521 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     2581 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     2641 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     2701 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     2761 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     2821 tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     2881 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     2941 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     3001 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     3061 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     3121 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     3181 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     3241 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     3301 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     3361 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     3421 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     3481 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     3541 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     3601 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     3661 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     3721 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     3781 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     3841 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     3901 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     3961 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     4021 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     4081 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
     4141 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
     4201 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
     4261 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
     4321 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     4381 tacgagacac ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag
     4441 aaggctggac aggctaagaa gaagaaagat tacaaagacg atgacgataa gggatccgga
     4501 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccagctagc
     4561 taaatccggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     4621 aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     4681 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     4741 aacgttcttt ttcgcaacgg gtttgccgcc agaacacaga ctgcgatcgc aatgtacagt
     4801 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4861 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4921 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4981 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     5041 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     5101 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     5161 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     5221 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     5281 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     5341 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccggt
     5401 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     5461 ccgatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt
     5521 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     5581 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     5641 ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg
     5701 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     5761 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     5821 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     5881 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     5941 acacctacca ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg
     6001 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     6061 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     6121 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     6181 gattttgacc ttgacatgct ccccgggtaa ctcgagcgcg ttaagtcgac aatcaacctc
     6241 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6301 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6361 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6421 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6481 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6541 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6601 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6661 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6721 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6781 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     6841 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6901 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6961 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7021 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7081 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7141 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7201 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7261 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7321 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7381 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7441 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7501 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7561 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7621 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7681 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7741 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7801 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7861 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7921 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7981 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8041 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8101 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8161 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8221 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8281 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     8341 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     8401 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     8461 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     8521 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8581 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8641 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8701 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8761 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8821 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8881 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8941 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9001 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9061 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9121 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9181 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9241 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9301 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9361 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9421 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9481 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9541 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9601 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9661 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9721 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9781 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9841 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9901 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9961 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10021 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10081 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10141 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10201 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10261 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10321 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10381 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10441 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10501 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10561 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10621 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10681 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10741 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10801 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10861 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10921 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10981 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11041 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11101 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    11161 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    11221 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    11281 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    11341 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    11401 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
    11461 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    11521 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
    11581 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
    11641 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
    11701 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
    11761 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
    11821 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
    11881 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
    11941 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
    12001 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    12061 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    12121 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
    12181 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
    12241 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
    12301 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
    12361 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
    12421 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
    12481 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
    12541 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
    12601 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
    12661 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
    12721 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    12781 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    12841 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    12901 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    12961 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    13021 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    13081 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    13141 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    13201 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    13261 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    13321 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    13381 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    13441 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    13501 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
    13561 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    13621 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    13681 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattaa
    13741 ggtaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
    13801 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
    13861 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
    13921 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
    13981 aaaggacgaa acaccggaga cggttgtaaa tgagcacaca aaatacacat gctaaaatat
    14041 tatattctat gacctttata aaatcaacca aaatcttctt tttaataact ttagtatcaa
    14101 taattagaat ttttatgttc ctttttgcaa acttttaata aaaatgagca aaataaaaaa
    14161 acgctagttt tagtaactcg cgttgttttc ttcaccttta ataatagcta ctccaccact
    14221 tgttcctaag cggtcagctc ctgcttcaat cattttttga gcatcttcaa atgttctaac
    14281 tccaccagct gctttaacta aagcattgtc tttaacaact gacttcatta gtttaacatc
    14341 ttcaaatgtt gcacctgatt ttgaaaatcc tgttgatgtt ttaacaaatt ctaatccagc
    14401 ttcaacagct atttcacaag ctttcatgat ttcttctttt gttaataaac aattttccat
    14461 aatacattta acaacatgtg atccagctgc tttttttaca gctttcatgt cttctaaaac
    14521 taattcataa tttttgtctt ttaatgcacc aatatttaat accatatcaa tttctgttgc
    14581 accatcttta attgcttcag aaacttcgaa tgcttttgta gctgttgtgc atgcacctag
    14641 aggaaaacct acaacatttg ttattcctac atttgtgcct tttaataatt ctttacaata
    14701 gcttgttcaa tatgaattaa cacaaactgt tgcaaaatca aattcaattg cttcatcaca
    14761 taattgttta atttcagctt tcgtagcatc ttgttttaat aatgtgtgat ctatatattt
    14821 gtttagtttc attttttctc ctatatattc atttttaatt ttaattcttt aataatttcg
    14881 tctactttaa ctttagcgtt ttgaacagat tcaccaacac ctataaaata aatttttagt
    14941 ttaggttcag ttccacttgg gcgaacagca aatcatgact tatcttctaa ataaaatttt
    15001 agtaagtctt gtcctggcat attatacatt ccatcgatgt agtcttcaac attaacaact
    15061 ttaagtccag caatttgagt taagggtgtt gctctcaatg atttcattaa tggttcaatt
    15121 tttaatttct tttcttctgg tttaaaattc aagtttaaag tgaaagtgta atatgcaccc
    15181 atttctttaa ataaatcttc taaatagtct actaatgttt tattttgttt tttataaaat
    15241 caagcagcct ctgctattaa tatagaagct tgtattccat ctttatctct agctgagtca
    15301 tcaattacat atccataact ttcttcataa gcaaaaacaa aatttaatcc gttatcttct
    15361 tctttagcaa tttctctacc cattcattta aatccagtta aagtttttac aatattaact
    15421 ccatattttt catgagcgat tctatcaccc aaatcacttg ttacaaaact tgaatataga
    15481 gccggatttt ttggaatgct atttaagcgt tttagatttg ataattttca atcaattaaa
    15541 attggtcctg tttgatttcc atctaatctt acaaaatgac catcatgttt tattgccatt
    15601 ccaaatctgt cagcatctgg gtcattcata ataataatat ctgcatcatg tttaatacca
    15661 tattcaagcg gtatttttca tgcaggatca aattctggat ttggatttac aacattttta
    15721 aatgtttcat cttcaaatgc atgctcttca acctcaataa cgttatatcc tgattcacgt
    15781 aatatttttg gggtaaattt agttcctgtt ccattaactg cgctaaaaat aatttttaaa
    15841 tcttttttag cttcttgctc ttttttgtac gtctctgttt tagagctaga aatagcaagt
    15901 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttga
    15961 attcactttg gccgcgaatc gatatgtcga gtttactccc tatcagtgat agagaacgt
//
