LOCUS       sequence-257465-        9640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EXOC7 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, 
            Yu H, Shen J
  TITLE     Inducible Exoc7/Exo70 knockout reveals a critical role of the 
            exocyst in insulin-regulated GLUT4 exocytosis.
  JOURNAL   J Biol Chem. 2019 Dec 27;294(52):19988-19996. doi: 
            10.1074/jbc.RA119.010821. Epub 2019 Nov 18.
  PUBMED    31740584
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.010821"; journalName: "J Biol Chem"; date: 
            "2019-12-27- 27"; volume: "294"; issue: "52"; pages: "19988-19996"
COMMENT     Sequence Label: SHC003BSD-EXOC7
FEATURES             Location/Qualifiers
     source          1..9640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     426..444
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     LTR             989..1222
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1294..1415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1331..1350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1385..1404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1455..1590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1610..1629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1611..1629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1639..1656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1639..1655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1648..1670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1797..2252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1884..1903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2094..2115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2278..2382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2383..2451,2452..3243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2601..2620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3414..4002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3903..3922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4156..4173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4290..4311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4326..4331,4332..4349,4350..4356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4364..4380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4369..4391
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4388..4404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4388..4404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4423..4443
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4425..4443
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4471..4697
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4698..4878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4925..5050
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5544..5777
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5962..6006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6305..6553
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6305..6325
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6476..6495
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        6616..6919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6920..7123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7074..7094
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      7155..7164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7164..7229
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    9471..9588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9637..9640,1..507)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccctagc caccatgcct ttgtctcaag
      541 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
      601 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
      661 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
      721 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
      781 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
      841 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
      901 atgtgtggga gggctaaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
      961 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     1021 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     1081 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     1141 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     1201 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     1261 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     1321 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     1381 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     1441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     1501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     1561 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     1621 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     1681 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1741 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     1801 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     1861 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     1921 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     1981 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     2041 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2101 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     2161 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2221 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2401 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2461 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2521 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2581 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2641 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2701 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2761 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2821 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2881 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2941 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     3001 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     3061 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     3121 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     3181 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     3241 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3301 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3361 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3421 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3481 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3541 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3601 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3661 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3721 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3781 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3841 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3901 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3961 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4021 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4081 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4141 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4201 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4261 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4321 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4381 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     4441 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     4501 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     4561 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     4621 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     4681 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     4741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     4801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     4861 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     4921 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     4981 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     5041 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     5101 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     5161 acgattcgca gttaatcctg gcctgttaga aacatcacga aggctgtaga caaatactgg
     5221 gacagctaca accatccctt cagacaggat cagaagaact tacatcatta tataatacag
     5281 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     5341 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     5401 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     5461 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     5521 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     5581 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     5641 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     5701 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     5761 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     5821 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     5881 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     5941 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     6001 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaaat tattcataat
     6061 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6121 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     6181 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     6241 attagtgaac ggatctcgac ggtatcgatc acgagactag cctcgagcgg ccgccccctt
     6301 caccgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     6361 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     6421 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     6481 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     6541 aggacgaaac accggtagtt attaatagta atcaattacg gggtcattag ttcatagccc
     6601 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     6661 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     6721 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     6781 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     6841 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     6901 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     6961 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     7021 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     7081 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
     7141 gatccgctag cctagccacc atggactaca aagaccatga cggtgattat aaagatcatg
     7201 acatcgatta caaggatgac gatgacaaga tgattccccc acaggaggca tccgctcgac
     7261 ggcgggagat tgaggacaag ctgaagcagg aggaggagac tctgtccttc atccgagaca
     7321 gcctggagaa gagcgaccag ctcactaaga acatggtgtc tatcttatca tcctttgaga
     7381 gccgccttat gaagctggag aactccatca tccctgtgca caagcagacg gagaatctgc
     7441 agcggctgca ggagaatgtt gagaagacgc tgtcctgcct ggaccatgtc atcagctact
     7501 accatgtggc cagtgacact gagaagatca tcagagaggg ccccacaggt aggctggaag
     7561 agtacctggg aagcatggcc aagattcaga aggcagtgga gtatttccag gacaacagcc
     7621 cagacagccc ggaactcaac aaagtgaaac tgctctttga gcgcgggaag gaggccctgg
     7681 agtccgaatt tcgcagcctg atgacgcggc acagtaaggt cgtctcgccc gtgctcatct
     7741 tggatctgat cagtggtgac gatgatctgg aggcccagga ggacgtgacc ctggagcacc
     7801 tgcccgagag cgtgctccag gatgtcattc gcatctcccg ctggctggtg gaatatggcc
     7861 gcaaccaaga tttcatgaac gtctactacc agatacgctc cagccagctg gaccgctcca
     7921 tcaaaggact gaaggagcat ttccataaga gcagttcttc ctctggggtt ccctactccc
     7981 ctgctatccc caacaagagg aaagacacac ctaccaagaa gccagtcaag cggccaggga
     8041 cgatccgtaa ggctcagaac cttctgaaac agtattccca gcatggtcta gatgggaaaa
     8101 aggggggctc taacctcatt cctctggaag ggagagatga catgctggac gtggagaccg
     8161 atgcctacat ccactgcgtc agtgccttcg tcaagctggc gcagagcgag taccagctgc
     8221 tggccgacat catccccgag caccaccaga agaagacctt cgactccctg atacaggatg
     8281 ccctggatgg gctgatgctt gaaggggaga acatcgtgtc tgctgcccgg aaggccattg
     8341 tgcgacacga cttctccacg gtgctcaccg tcttccccat cctgcgacac ctcaagcaga
     8401 ccaagcctga gtttgaccag gtgctccagg gcacggctgc cagcacaaag aacaagctgc
     8461 ctggcctcat cacatccatg gagaccatcg gtgccaaagc gctggaggac ttcgcagaca
     8521 acatcaagaa tgacccggac aaggagtaca acatgccgaa ggacggcacc gtacacgagc
     8581 tcaccagcaa tgccatcctc ttcctgcagc agcttttgga cttccaggag acggcaggcg
     8641 ccatgctggc ctcccaagag accagctctt cggccaccag ctacagctct gagttcagca
     8701 agcggctgct aagcacctat atctgtaaag tgctgggcaa cctgcagttg aacttgctga
     8761 gcaagtccaa ggtgtacgag gacccagctc tgagcgccat cttcctgcac aacaactaca
     8821 attacatcct caagtccctg gagaagtctg aactgatcca gctggtggca gtgacacaga
     8881 agactgctga gcgctcctac cgggagcaca ttgagcagca gatccagacc taccagcgca
     8941 gctggttaaa ggtgactgat tacatcgcag agaagaatct acctgtgttc cagccgggag
     9001 tcaagctccg ggacaaggag cggcagatta tcaaggagcg ttttaagggc ttcaatgatg
     9061 gcctcgaaga actgtgcaaa atccagaagg cctgggctat tccagacaca gagcagaggg
     9121 acaggattcg ccaggcccag aagaccattg tcaaggagac ctacggggcc tttctacaga
     9181 agtttggcag cgtgcccttc accaagaacc cggagaagta catcaagtac ggggtggagc
     9241 aggtgggcga catgatcgat cgccttttcg acacctctgc ctaagtcgac ccccagcatc
     9301 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggaggatca cagcaacacc
     9361 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc cggatgcaga tgccggtgaa
     9421 gaataaaatt aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
     9481 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9541 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9601 agggacagca gagatccact ttggccgcgg ctcgaggggg
//
