LOCUS       sequence-257466-        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His6-Ubc9-C138A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Hofmann R, Akimoto G, Wucherpfennig TG, Zeymer C, Bode JW
  TITLE     Lysine acylation using conjugating enzymes for site-specific 
            modification and ubiquitination of recombinant proteins.
  JOURNAL   Nat Chem. 2020 Nov;12(11):1008-1015. doi: 10.1038/s41557-020-0528-y.
            Epub 2020 Sep 14.
  PUBMED    32929246
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-020-0528-y"; journalName: "Nat Chem"; date: 
            "2020-11"; volume: "12"; issue: "11"; pages: "1008-1015"
COMMENT     Sequence Label: pET28-His6-Ubc9-C138A
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             62..79
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             569..586
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(639..657)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      653..700
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(824..843)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aactttaaga aggagatata ccatgggcag cagccatcat catcatcatc acagcagcgg
       61 cctggtgccg cgcggcagcc atatgatgtc cggaattgcc ctctctcgct tagctcagga
      121 acgcaaagcg tggcgcaaag atcacccgtt tgggtttgtt gccgtgccta ccaaaaatcc
      181 tgacggtacg atgaatctta tgaactggga atgcgcgatc cctggcaaga aaggcacacc
      241 gtgggaaggc ggcttgttca agctgcgtat gttgttcaaa gatgattacc cgtcaagtcc
      301 gcctaaatgt aaattcgagc cgccgttatt tcatccaaac gtgtatccca gcggtacggt
      361 ttgcctgagc atccttgaag aagacaaaga ttggcgtccc gcaattacca tcaagcagat
      421 tctgctcggc attcaagagc tgttaaatga accaaacatt caggacccag cgcaggcaga
      481 agcctatact atctacgcgc agaaccgtgt agagtatgag aagcgcgtcc gtgcacaagc
      541 gaaaaagttt gccccgtcgt aactcgagca ccaccaccac caccactgag atccggctgc
      601 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      661 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      721 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgkggtggtt
      781 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      841 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      901 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      961 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1021 acgttcttta atagtggact cttgttccaa actg
//
