LOCUS       sequence-25753-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Tadeo X, Lopez-Mendez B, Trigueros T, Lain A, Castano D, Millet O
  TITLE     Structural basis for the aminoacid composition of proteins from 
            halophilic archea.
  JOURNAL   PLoS Biol. 2009 Dec;7(12):e1000257. doi: 
            10.1371/journal.pbio.1000257. Epub 2009 Dec 15.
  PUBMED    20016684
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2009-12"; volume: "7(12):e1000257. doi"; pages: " 
            10.1371/journal.pbio.1000257. Epub 2009 Dec 15"
COMMENT     Sequence Label: pDEST17-1ALigN
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(380..404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(457..475)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      471..518
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 caggnttcnn ctgaaatatt ttgtttactt taagaaggag atatacatat gtcgtactac
       61 catcaccatc accatcacct cgaatcaaca agtttgtaca aaaaagcagg cttccctccg
      121 accgagttcg aacccgccga gtcgctctcg cgcgaggcgg ccgaagggca ggccgcactc
      181 ctccgcgagg ccgtccgtga gcacgaccac cgctactacg tcgcggccga cccgctcgtc
      241 tccgacgcgg cctacgacgc gctgttctcc cgactcgtcg cgctcgaaga cgcgttcgac
      301 ctcgacacga ccaacagccc gacgaaccgc gtcggcggcg agcccatcga cgcgctggag
      361 acggtcgaac acgtctagga cccagctttc ttgtacaaag tggttgattc gaggctgcta
      421 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      481 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      541 gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc
      601 gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccaag aangggtgcg
      661 catagaaatt gtatcaacgc ttatagcgtn tagcagcacg cnaatagtga ctggcgatgc
      721 tgtcggaatg gacgatatcc cgcaagaggc cggcagtacc ggcataccaa gctatgctac
      781 ngcatcaggt gacgtgccaa gatanaataa ncntnnttat ttntaccngn c
//
