LOCUS       sequence-257533-        9141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PDZ mCherry DrTrkA expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Leopold AV, Chernov KG, Shemetov AA, Verkhusha VV
  TITLE     Neurotrophin receptor tyrosine kinases regulated with near-infrared 
            light.
  JOURNAL   Nat Commun. 2019 Mar 8;10(1):1129. doi: 10.1038/s41467-019-08988-3.
  PUBMED    30850602
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-03-8"; volume: "10(1):1129. doi"; pages: " 
            10.1038/s41467-019-08988-3"
COMMENT     Sequence Label: pPDZ-mCherry-Dr-TrkA
FEATURES             Location/Qualifiers
     source          1..9141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1207..1914
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1352..1370)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1620..1639
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(4694..4712)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4695..4712)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4732..4749)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4738..4962
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5008..5436
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5095..5114)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5305..5326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5445..5465)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5450..5779
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5630..5765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5692..5711
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5846..6640
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5900..5919)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6510..6529
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6814..6935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6851..6870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6905..6924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6984..7000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6984..7000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6997..7019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7008..7024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7032..7038,7039..7056,7057..7062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7077..7098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7215..7232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7386..7974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7466..7485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8145..8936,8937..9005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8768..8787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9006..9110)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatggca
      901 aaacaagaga ttcgagtgag ggttgaaaag gacccagaac ttggatttag catatcaggt
      961 ggtgtcgggg gtagaggaaa cccattcaga cctgatgatg atggtatatt tgtaacaagg
     1021 gtacaacctg aaggaccagc atcaaaatta ctgcagccag gtgataaaat tattcaggct
     1081 aatggctaca gttttataaa tattgaacat ggacaagcag tgtccttgct aaaaactttc
     1141 cagaatacag ttgaactcat cattgtacga gaagtttcta gactcgagag cgcgggcagc
     1201 gcgggcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     1261 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     1321 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     1381 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     1441 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     1501 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     1561 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     1621 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     1681 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     1741 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     1801 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1861 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggaattc
     1921 agtgctggta gtgctggtag tgctggcacc ggtatgagcc gggacccgtt gccctttttt
     1981 ccaccgcttt accttggtgg cccggaaatt accaccgaga actgcgagcg cgagccgatt
     2041 catattcccg gcagcatcca gccgcacggc gccctgctca ctgccgacgg gcacagcggc
     2101 gaggtgctcc agatgagcct caacgcggcc acttttctgg gacaggaacc cacagtgctg
     2161 cgcggacaga ccctcgccgc actgctgccc gagcagtggc ccgcgctgca agcggccctg
     2221 ccccccggct gccccgacgc cctgcaatac cgcgcaacgc tggactggcc tgccgccggg
     2281 cacctttcgc tgacggtgca ccgggtcggc gagttgctga ttctggagtt cgagccgacg
     2341 gaggcctggg acagcaccgg gccgcacgcg ctgcgcaacg cgatgttcgc gctcgaaagt
     2401 gcccccaacc tgcgggcgct ggccgaggtg gcgacccaga cggtccgcga gctgacgggc
     2461 tttgaccggg tgatgctcta caaatttgcc cccgacgcca ccggcgaagt gattgccgag
     2521 gcccgccgtg aggggctgca cgcctttctg ggccaccgtt ttcccgcgtc ggacattccg
     2581 gcgcaggccc gcgcgctcta cacccggcac ctgctgcgcc tgaccgccga cacccgcgcc
     2641 gccgccgtgc cgctcgatcc cgtcctcaac ccgcagacga atgcgcccac cccgctgggc
     2701 ggcgccgtgc tgcgcgccac ctcgcccatg cacatgcagt acctgcggaa catgggcgtc
     2761 gggtcgagcc tgtcggtgtc ggtggtggtc ggcggccagc tctggggcct gatcgcctgc
     2821 caccaccaga cgccctacgt gttgccgccc gacctgcgaa ccacgctcga atacctgggc
     2881 cgcttgctga gcctgcaagt tcaggtcaag gaagcggcgg acgtggcggc ctttcgccag
     2941 agcctgcggg agcaccacgc gcgggtggcc ctcgcggcgg cgcactcgct ctcgccgcac
     3001 gacaccctca gtgacccggc gcttgacctg ctgggcctga tgcgggccgg gggcctgatt
     3061 ctgcgtttcg agggccgctg gcagacgttg ggtgaagtgc cgcctgcccc ggcggtggac
     3121 gcgctgctgg cgtggctcga aacccagccg ggcgccctgg tccagaccga cgcgctaggc
     3181 caactgtggc ccgccggcgc cgatctcgcc cccagcgcag cgggcctgct cgccatcagc
     3241 gtgggcgagg gctggtcgga gtgcctcgtc tggctgcggc ccgaactgcg gctggaggtc
     3301 gcctggggcg gggccactcc tgaccaggcg aaagacgacc tcgggccgcg ccactcattc
     3361 gacacctacc tcgaagaaaa acgcggctac gccgagccct ggcatcccgg cgaaatcgag
     3421 gaggcgcagg atctacgtga cacattgacc ggggcgctgg gcgaggcaga ggcagcagca
     3481 aaggaggcag cagcaaagga agcggcggcg aaagaagcgg cggcgaaagc gaacaaatgt
     3541 ggacagagga gcaaatttgg gatcaaccgc cctgctgtgc tggcgccaga ggatgggctg
     3601 gccatgtccc tacacttcat gacactgggt ggcagttctc tttcccctac tgagggcaaa
     3661 ggctccggac tccagggcca catcatggag aacccacagt acttcagtga tacctgtgtc
     3721 caccatatca agcgccagga catcattctc aagtgggagc taggggaggg agcctttgga
     3781 aaggtctttc ttgctgagtg ctacaacctt ctgaatgatc aggacaagat gctggtggct
     3841 gtcaaggcac tgaaggagac atctgagaat gctcgtcagg acttccatcg tgaggcagag
     3901 ctgctcacca tgctacagca ccaacacatc gtacgcttct ttggagtctg cacggagggt
     3961 ggcccattgc tcatggtctt cgagtacatg cgccatgggg acctcaaccg tttcctccgg
     4021 tcccacggac ctgatgcaaa actgctggct ggcggcgagg atgtggctcc tggtcctttg
     4081 ggccttgggc agcttctggc tgtggctagc caggtggctg ctggtatggt gtacctagcc
     4141 agcctgcact ttgtgcaccg ggacctggcc acacgcaact gtctggtggg tcagggacta
     4201 gtggtcaaga ttggagactt cggcatgagc agggacatct acagcacaga ctactaccgt
     4261 gtgggaggtc ggaccatgct gcccatccgc tggatgcctc cagagagcat cctctaccgc
     4321 aagttcagca ccgagagtga tgtgtggagc ttcggggtgg tgctctggga gatcttcacc
     4381 tatggaaagc aaccctggta ccagctctcc aacactgagg cgatcgagtg catcacgcag
     4441 ggccgggagc tggagcggcc gcgcgcctgc cctcctgatg tctacgccat catgcgcggc
     4501 tgctggcagc gtgagccgca acagcgcctc agcatgaagg atgtgcacgc gcggctgcag
     4561 gccttggcac aggcgccacc gagttacctg gacgttctgg gcggatccac tagtaacggc
     4621 cgccagtgtg ctggaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc
     4681 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     4741 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     4801 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     4861 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     4921 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     4981 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5041 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5101 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5161 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5221 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5281 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5341 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5401 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5461 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5521 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5581 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5641 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5701 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     5761 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     5821 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     5881 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5941 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6001 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6061 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6121 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6181 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6241 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6301 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6361 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6421 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6481 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6541 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6601 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6661 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6721 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6781 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6961 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9121 cgaaaagtgc cacctgacgt c
//
